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| Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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01-24-2010, 08:05 AM
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#1 (permalink) |
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Tissue Culturist
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step by step Tissue Culture Pineapple
I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.  step 2. throw out the leaf until the last/youngest leaf  step 3. soak in bleach 10 % for 10 minute  step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.  step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA  step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2  step 7. in 2 next month shoot will growth. very beautiful. isnt it?  regards
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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01-24-2010, 08:07 AM
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#2 (permalink) |
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Re: step by step Tissue Culture Pineapple
wooooooo very interesting novi!!!! i will have to do this some time!
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01-24-2010, 08:56 AM
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#3 (permalink) |
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Re: step by step Tissue Culture Pineapple
Very cool! Thanks for posting this. I may have to try this now someday.
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01-24-2010, 09:03 AM
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Re: step by step Tissue Culture Pineapple
Thank you for posting this Novisyatria!! That is good information.
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01-24-2010, 02:59 PM
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#5 (permalink) |
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Re: step by step Tissue Culture Pineapple
WONDERFUL NOVI!!!
Thank you!!!  ~Cheryl
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01-24-2010, 06:29 PM
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#6 (permalink) |
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Re: step by step Tissue Culture Pineapple
Yes very cool, I would love to try it Thanks Novi
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01-24-2010, 07:07 PM
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#7 (permalink) |
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Re: step by step Tissue Culture Pineapple
awsome, thanks for sharing
What do you do after step 7... Do you cut the growth off at its base and put it in rooting hormone, then soil, Or do you cut off the growth with a little bit of the base left and put it in rooting hormone... Thanks for helping me
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 A banana plant in the autumn gale - I listen to the dripping of rain Into a basin at night. ~ Basho Matsuo (1644- 1694)  
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01-25-2010, 07:24 AM
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#8 (permalink) |
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Tissue Culturist
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Re: step by step Tissue Culture Pineapple
blake, i can do both. move the shoots in to rooting media and than in soil. and also can cut the shoot and put in same media for shoot multification. so i can still have shoots stock.
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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01-30-2010, 10:55 PM
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#9 (permalink) |
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Re: step by step Tissue Culture Pineapple
Great!!
Thanx 4 sharing pak novi  knowledge is power!! |
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02-07-2010, 09:52 AM
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#10 (permalink) |
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Re: step by step Tissue Culture Pineapple
Thanks Novi! This is great. I'm going to try this today!
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02-07-2010, 04:05 PM
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#11 (permalink) |
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Re: step by step Tissue Culture Pineapple
Thank You Novi! I have several varieties that I will be mass propagating very soon. Your pictures have helped me understand the process much better than I did after reading about it. I will also start my tc's using the same media formulations that you prescribe here. Thanks again!
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02-07-2010, 04:59 PM
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#12 (permalink) |
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Re: step by step Tissue Culture Pineapple
Cool!!! It's just like TC'ing Bananas!
I'm working on my pineapple right now. I'll post some picts in a few minutes. Thanks again Novi!!! This is awesome!
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02-07-2010, 05:40 PM
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#13 (permalink) | ||
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Tissue Culturist
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Re: step by step Tissue Culture Pineapple
Quote:
Quote:
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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02-07-2010, 06:08 PM
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#14 (permalink) |
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Re: step by step Tissue Culture Pineapple
Okay, I'm done cutting the pineapple up. And to think there is a ham in the freezer
 I had this growing in a pot outside. I cut the top off of a pineapple and stuck it ontop of of some dirt in the pot. The frost got to it. I thought it was a goner. The outer leaves died but the inner leave survived. Here is the sad looking pineapple. At least it gave me 10 explants!  Sad Pineapple  Cleaning of the dirt  There's something alive under all of that   Hacking away at it.  It tastes like chicken   Into the disinfecting solution  10 Explants   Thanks Novi!!!!!
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Frankco Zone 5 N.W. Indiana |
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02-07-2010, 07:56 PM
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#15 (permalink) |
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Tissue Culturist
Location: Jakarta, Indonesia
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Re: step by step Tissue Culture Pineapple
cool pic Frank. good luck. and post after the explantgrowth ok
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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02-07-2010, 08:07 PM
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#16 (permalink) |
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Re: step by step Tissue Culture Pineapple
Hi Novi,
Will do. That was fun! Now to see if they grow Like you said, it is a thing of beauty to see plants grow from little pieces. Now I'm going try caladiums. Your pictures of them look fantastic.
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Frankco Zone 5 N.W. Indiana |
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02-07-2010, 11:01 PM
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#17 (permalink) |
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Re: step by step Tissue Culture Pineapple
This is a great thread!.....keep up the good work guys
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03-01-2010, 05:54 PM
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#18 (permalink) |
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Re: step by step Tissue Culture Pineapple
Sorry everybody. I´m still out at the countryside: The Great Savannah!
I´ll post some pics next week. Great job Novi! Now I´m in a rush to get home ( that´s where I have my products ) Thanks a lot. |
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07-27-2010, 01:27 PM
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#19 (permalink) |
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Re: step by step Tissue Culture Pineapple
I wanna do tissue culture of pineapples and was wondering were to buy the little jars and from what I could find the media that novi refers to is different kind of hormones.
Please help me in successful tissue culture thanks Brian
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07-27-2010, 03:19 PM
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#20 (permalink) |
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Re: step by step Tissue Culture Pineapple
Hi Brian,
Canning jars work well. As for the media and plant growth regulators, you can get them from Carol at Home Tissue Culture* Group I think you will find her shipping charges much lower than the TC supply houses. You will find her email address on the site if you have any questions. Frank
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