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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 10-07-2009, 03:14 AM   #1 (permalink)
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Drummer Step by Step Subculture In Tissue CUlture Bananas (part 1)

Hi all
I promise you all to take photo subculture in TC bananas step by step. thats why i I'v done today. enjoy the pic.

Step 1. Explant banana 1month old. ready for subculture 1 (Photo 1)
Step 2. Put the explant on sterile petri dish/plate (Photo 2)
Step 3. Cut vertically become 2 explant (Photo 3)
Step 4. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo4)
Step 5. After 1 month next, explant has grow and ready for subculture 2. explant will have few baby bananas. about4-7 baby (photo 5)
Step 6. Put the explat on sterile petridish (Photo 6)
Step 7. Cut the explant become few. every parts could be consist of 2-3 baby bananas (Photo 7)
Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8). Look at the next thread
Next Step like subculture 1 or subculture 2
Attached Images
File Type: jpg IMGP2243 Step 1 Ready for subculture 1.JPG (78.4 KB, 200 views)
File Type: jpg IMGP2245 Step 2.JPG (81.9 KB, 174 views)
File Type: jpg IMGP2246 step 3.JPG (63.8 KB, 187 views)
File Type: jpg IMGP2247 Step 4.JPG (76.4 KB, 175 views)
File Type: jpg IMGP2244 Step 5 Ready for subculture 2.JPG (77.3 KB, 191 views)
File Type: jpg IMGP2249 Step 6.JPG (70.0 KB, 169 views)
File Type: jpg IMGP2250 Step 7.JPG (66.6 KB, 180 views)
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Old 10-07-2009, 11:18 AM   #2 (permalink)
I think with my banana ;)
 
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

A thread many of us have been waiting for. Thanks for this one, pal.
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Old 10-07-2009, 12:11 PM   #3 (permalink)
 
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

That's great!....Thanks for taking the time to share the information and photos.
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Old 10-07-2009, 04:27 PM   #4 (permalink)
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

Very Nice!
Could you give us a little more detail though about each stage, and how you got there, and to the next stage?

Kind regards,
Remko.
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Old 10-07-2009, 07:20 PM   #5 (permalink)
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

Hi Remko
before this thread i hav been post step by step how TC bananas. just look in TIssue Culturing Forum jump

regrads
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Old 10-07-2009, 08:41 PM   #6 (permalink)
 
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

sweet@!
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Old 10-09-2009, 02:17 PM   #7 (permalink)
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

Awesome !!!
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Old 04-24-2019, 10:04 PM   #8 (permalink)
 
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

I am facing problems in the shoot multiplication step.Can you please help me out why there is no growth even after adding adequate hormones and frequent subcultures?
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Old 04-30-2019, 09:03 PM   #9 (permalink)
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Default Re: Step by Step Subculture In Tissue CUlture Bananas (part 1)

Quote:
Originally Posted by Indrani Chetia View Post
I am facing problems in the shoot multiplication step.Can you please help me out why there is no growth even after adding adequate hormones and frequent subcultures?
hi my WA no +62811732212

thanks
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