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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 07-27-2010, 08:15 PM   #21 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Quote:
Originally Posted by Frankco View Post
Hi Brian,

Canning jars work well.

As for the media and plant growth regulators, you can get them from Carol at Home Tissue Culture Group

I think you will find her shipping charges much lower than the TC supply houses. You will find her email address on the site if you have any questions.

Frank
Have you successfully used any of these products for pineapple tissue culture?If not which would you or anyone that has used any of there products
suggest?Other than the hormones would you or anyone recommend anything else?With the jars what's the best method of keeping everything sterile from start to finish?
Thanks for EVERYONE'S help to a newbie,and yes I've done a little research first.
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Old 07-28-2010, 02:21 PM   #22 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

You can buy a lot of the growth regulators from this guy Benzylaminopurine they come as powders so you need a bit of know how as far as chemistry goes and getting them to dissolve is a bit of a pain in the backside, but it's by far the cheapest way of doing things.

The agar is a vegetarian alternative to gelatine and can be purchased from health food shops or on line. As for the containers, yes canning jars or Kilner jars are a good thing.
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Old 07-28-2010, 02:34 PM   #23 (permalink)
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Default Re: step by step Tissue Culture Pineapple

I have used baby food jars.
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Old 07-28-2010, 08:18 PM   #24 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

do i need anything other than the hormones,I dont understand agar either?
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Old 07-28-2010, 08:42 PM   #25 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Hi Pineapplefarmer,

I'm following Novi's protocol using MS, Murashige & Skoog Basal Medium, that's the nutrients, along with 5 mg/L NAA and 2 mg/L BAP.

NAA is 1-Naphthaleneacetic acid. BAP is 6-Benzylaminopurine. These are the two plant growth regulators you need to add to the media. You can get this already in a solution so adding them to the media isn't very difficult.

Agar, like jmoor said, can be found in health food stores. I purchased 56 grams from Amazon for less than $5. You only need to add 7.5 grams of powered agar per liter of media so it will make a fair amount. It's pretty difficult to go through all of the steps to make the media in a post. These are getting old but I did put some videos on YouTube about my experience getting started with tissue culture. Maybe they would help.
YouTube - fbt2007's Channel

I use canning jars for bananas and other large plants. They work nice because of the extra headroom. If you use them, drill or puch a small 1/8th inch hole in the lid and cover it with a round band-aid. This allows for air exchange. Cover the lid with a wrap of aluminum foil to help keep contamination from settling on the band-aid and getting in the jar.




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Old 07-28-2010, 09:43 PM   #26 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Thanks so much

Quote:
Originally Posted by Frankco View Post
Hi Pineapplefarmer,

I'm following Novi's protocol using MS, Murashige & Skoog Basal Medium, that's the nutrients, along with 5 mg/L NAA and 2 mg/L BAP.

NAA is 1-Naphthaleneacetic acid. BAP is 6-Benzylaminopurine. These are the two plant growth regulators you need to add to the media. You can get this already in a solution so adding them to the media isn't very difficult.

Agar, like jmoor said, can be found in health food stores. I purchased 56 grams from Amazon for less than $5. You only need to add 7.5 grams of powered agar per liter of media so it will make a fair amount. It's pretty difficult to go through all of the steps to make the media in a post. These are getting old but I did put some videos on YouTube about my experience getting started with tissue culture. Maybe they would help.
YouTube - fbt2007's Channel

I use canning jars for bananas and other large plants. They work nice because of the extra headroom. If you use them, drill or puch a small 1/8th inch hole in the lid and cover it with a round band-aid. This allows for air exchange. Cover the lid with a wrap of aluminum foil to help keep contamination from settling on the band-aid and getting in the jar.




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Old 07-29-2010, 10:19 PM   #27 (permalink)
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Default Re: step by step Tissue Culture Pineapple

Frank
can you upload new pic of your pineapples Tc? btw i have 8 type now in jars
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Old 07-29-2010, 10:36 PM   #28 (permalink)
 
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Joy Re: step by step Tissue Culture Pineapple

Quote:
Originally Posted by novisyatria View Post
Frank
can you upload new pic of your pineapples Tc? btw i have 8 type now in jars
you have 8 different types of pineapples?
I want one of each type
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Old 07-31-2010, 08:34 PM   #29 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Does it matter if you the concentrated media or one with or without nutrients?
theres like a whole page of them...
link

MS-Based Salts & Media | PhytoTechnology Laboratories
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Old 09-11-2010, 09:06 AM   #30 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Hi Novi!!!

I had a hard time finding a nice "green" pineapple. I bough a couple that were too ripe and didn't realize it until I tried to trim them.

I really want to say THANK YOU!!! for your help with this. My pineapple explant has started to put on new leaves. I probably should have sliced it in half or quartered it like you did. Next time. For now I'll just enjoy watching this one develop.

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Old 03-14-2011, 10:49 PM   #31 (permalink)
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Default Re: step by step Tissue Culture Pineapple

Franck: congrats. please upload new pic
pineapplefarmerwannabe: i want sell to you a pineapple plantlet but hard to send it to overseas
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Old 03-15-2011, 01:48 AM   #32 (permalink)
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Default Re: step by step Tissue Culture Pineapple

An experienced pineapple grower that lives nearby told me that rather than planting the 'crown' that you cut off the pineapple, get one of the plantlets that develop between the upper leaves just below the fruit. Once those are planted, they develop into a mature plant much more quickly than those grown from the crown.
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Old 03-15-2011, 07:53 AM   #33 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Hi Novi,

Here's some picts of my baby pineapples. When I divided the original one up I put a couple of young leaves in jars. Those started to develop callus and multiply too. I read it was done before but was happy to see it.

These are still in the multiplying stage. After I get enough I'll put them in the shoot enlongation stage.







Thanks Novi!!!
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Old 05-25-2011, 12:10 AM   #34 (permalink)
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Default Re: step by step Tissue Culture Pineapple

you did it Frank. congrats.
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Old 05-25-2011, 08:28 AM   #35 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

I would have never figured out how much NAA to use without your help.
Thanks so much for your help.
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Old 01-05-2012, 04:48 PM   #36 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Bravooo.. and thanks for your pics... iíll try to TC pineapple first
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Old 01-17-2012, 10:42 PM   #37 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

I watched a youtube video of a pineapple grown from seeds. What type of pineapple do you get from a seed. Is it the same as the mother plant?
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Old 01-17-2012, 10:57 PM   #38 (permalink)
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Default Re: step by step Tissue Culture Pineapple

if from seeds could be different from its mother plant
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Old 08-01-2012, 08:46 PM   #39 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Trying pineapple tissue culture. The bottom of the explant turns brown, and top green is this normal? The samples are only 2 weeks old.
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Old 03-01-2013, 02:44 AM   #40 (permalink)
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Default Re: step by step Tissue Culture Pineapple

which one is it is the meristem. im doing tissue culture right now and im confuse which part of the pineapple to be cultured in the media.
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