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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 10-07-2009, 03:17 AM   #1 (permalink)
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Drummer Step by Step Subculture In Tissue Culture Bananas (part 2)

Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8).
Next Step like subculture 1 or subculture 2

I hope can help you in TC bananas through this thread.

Regards From Jakarta
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File Type: jpg IMGP2251 Step 8.JPG (102.5 KB, 220 views)
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Old 10-07-2009, 11:18 AM   #2 (permalink)
I think with my banana ;)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Beautiful.
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Old 10-16-2009, 01:17 PM   #3 (permalink)
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

novisyatria,

You make it seem so easy, thanks for taking the time.

Michael
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Old 09-02-2012, 10:48 AM   #4 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Quote:
Originally Posted by novisyatria View Post
Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8).
Next Step like subculture 1 or subculture 2

I hope can help you in TC bananas through this thread.

Regards From Jakarta
what size are your jars? where did you get them?
Thanks.
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Old 09-02-2012, 01:45 PM   #5 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Novi hasn't posted in quite a while so probably wont answer your post, but phytotech do culture jars I'm sure or you can use baby food jars with lids called magenta B caps available from sigma-aldrich.
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Old 09-02-2012, 02:45 PM   #6 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Quote:
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Novi hasn't posted in quite a while so probably wont answer your post, but phytotech do culture jars I'm sure or you can use baby food jars with lids called magenta B caps available from sigma-aldrich.
I've been using baby food jars but not digging the cap that come with them.
Thanks!
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Old 02-14-2013, 10:48 PM   #7 (permalink)
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

I think I can help you brother but I'm going to need your help in the future when I start tc haha so Cook Pro 6-Piece Food Storage Set: Kitchen & Dining : Walmart.com I've herd these are pretty damn good to use. Culture Vessels | PhytoTechnology Laboratories This is the fancy stuff here
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Old 08-11-2015, 09:19 AM   #8 (permalink)
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Hi everyone
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Old 08-12-2015, 07:01 AM   #9 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

Hi welcome back! I've missed you and your wonderful pictures are you still doing TC?
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Old 08-12-2015, 04:40 PM   #10 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

FYI: no need to buy expensive culture vessels or even baby jars for that matter. If you can find ziplock "microwave safe" square containers, it's a fraction of the cost. Square contains are ideal since they use up less space comapred to the circular ones. These can be pressure cooked, and re-used 2-3 times. You'll need a thick layer of parafilm to seal the tops but other than that, it's really easy to use.
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Old 06-13-2016, 05:04 AM   #11 (permalink)
 
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Default Re: Step by Step Subculture In Tissue Culture Bananas (part 2)

l want to thank Novi for her piece , until l saw her photos of banana TC l was struggling to take off but they eventually did. My biggest challenge was bacteria and browning , working with Williams so kept trying until lady luck smiled on me.
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