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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 09-28-2009, 09:51 PM   #1 (permalink)
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Default Step by Step How To Tissue Culturing Bananas

step 1. Prepare the corm
step 2 scarep out skin (sorry i dont know right term)
step 3 soak in bayklin (bleach) 25-30 minute and give it shake
sometime
step 4. rinse with sterile water 5 times
step 5 put on sterile petri dish or plate. and reary to cut. prepare the tools before like pinset, scapel/knife, bunsen fire dll
step 6. scarep out layer of stem has died (because bleach) aorund the explant
step 7 cut vertically become 2 explant
step 8 each explant put/planted in the culture bottle that has been prepared before. use MS media with 3-5 mg B enzyl amino purine (BAP)/liter solution
step 9. after 2 weeks explant growth
step 10 after 1 month explant ready for subculture
Attached Images
File Type: jpg IMGP2160 step 1.JPG (57.3 KB, 220 views)
File Type: jpg IMGP2161 step 2.JPG (61.1 KB, 221 views)
File Type: jpg IMGP2162 step 3.JPG (85.4 KB, 195 views)
File Type: jpg IMGP2163 step 4.JPG (92.0 KB, 178 views)
File Type: jpg IMGP2164 step 5.JPG (105.8 KB, 204 views)
File Type: jpg IMGP2165 step 6.JPG (66.6 KB, 195 views)
File Type: jpg IMGP2166 step 7.JPG (69.2 KB, 229 views)
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Old 09-29-2009, 02:54 AM   #2 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

thanks...

You have done a great job in your lab. Those of us that are learning appreciate your posts

aaron
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Old 09-29-2009, 09:35 AM   #3 (permalink)
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Default Re: Step by Step How To Tissue Culturing Bananas

This is Excellent information for people like me.
Thank you
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Old 09-29-2009, 02:10 PM   #4 (permalink)
Got pink bananas?
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Thanks for the pictures. I have always wondered how it was done.
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Old 09-29-2009, 07:47 PM   #5 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Could you post photos of the subculture process please. Thanks for the information.
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Old 09-29-2009, 08:05 PM   #6 (permalink)
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Default Re: Step by Step How To Tissue Culturing Bananas

ok I will post soon.
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Old 09-29-2009, 09:01 PM   #7 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Wow thanks! You saved me hours of reading mile long page after page...
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Old 09-29-2009, 09:16 PM   #8 (permalink)
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Default Re: Step by Step How To Tissue Culturing Bananas

dear blake
Yes if we reading textbook we needs much time. but what i post is very simple and practically. ihope you succes star with TC
regards
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Old 09-29-2009, 09:27 PM   #9 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Your very appreciated! ~JaNan
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Old 09-29-2009, 10:24 PM   #10 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Thanks so much for this thread! it is simple and helps me out alot Novi roxx! hehe!
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Old 09-30-2009, 12:38 AM   #11 (permalink)
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Default Re: Step by Step How To Tissue Culturing Bananas

kool i hope some day to do it too.
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Old 03-23-2012, 02:13 AM   #12 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Dear Novi,

Thank you for your step by step guide on TC of bananas.

I am new in this forum with some basic knowledge of TC and was looking for help on doing TC of banana at home.

Based on your step by step guide I have some questions:-


1. What is the ideal length and diameter of the suckers ?

2. Once I get the suckers (say from site of cultivation) , how long can they be stored at home before TC ?

3. I guess I may have to store it minimum 1 day at home before doing TC. How should I store them during this intermediate time ?

4. Is there any basic guidelines on preparing the crom ? Like how much to cut from the base and how much to cut from the top part (process between image 1 to image 2)?

5. What should be the ideal / average size of the prepared crom (step 1,image 2 )?

6. If I am doing TC of more than 1 sucker at one go (since I will prepare a min. 500ml or 1lit media), what is the sequence for sterilization ?
Example :- If I have 10 suckers, do I need 10 different bottles of bleach and 10 different bottles of sterile water ?
Or should I put all 10 into one large container containing bleach and another containg sterile water ?

7. Which part of the sterilization process can be done outside the hood and which part inside the hood ?

8. What should be the ideal / average size of explant before dividing into two (after step 6, image 6)?


I am not clear regarding the above. Clarifications on the above doubts would be of great help.

Thanks in advance.

Regards,
Amit


Quote:
Originally Posted by novisyatria View Post
step 1. Prepare the corm
step 2 scarep out skin (sorry i dont know right term)
step 3 soak in bayklin (bleach) 25-30 minute and give it shake
sometime
step 4. rinse with sterile water 5 times
step 5 put on sterile petri dish or plate. and reary to cut. prepare the tools before like pinset, scapel/knife, bunsen fire dll
step 6. scarep out layer of stem has died (because bleach) aorund the explant
step 7 cut vertically become 2 explant
step 8 each explant put/planted in the culture bottle that has been prepared before. use MS media with 3-5 mg B enzyl amino purine (BAP)/liter solution
step 9. after 2 weeks explant growth
step 10 after 1 month explant ready for subculture
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Old 04-10-2012, 08:35 PM   #13 (permalink)
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Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by ab2710 View Post
Dear Novi,

Thank you for your step by step guide on TC of bananas.

I am new in this forum with some basic knowledge of TC and was looking for help on doing TC of banana at home.

Based on your step by step guide I have some questions:-


1. What is the ideal length and diameter of the suckers ?

2. Once I get the suckers (say from site of cultivation) , how long can they be stored at home before TC ?

3. I guess I may have to store it minimum 1 day at home before doing TC. How should I store them during this intermediate time ?

4. Is there any basic guidelines on preparing the crom ? Like how much to cut from the base and how much to cut from the top part (process between image 1 to image 2)?

5. What should be the ideal / average size of the prepared crom (step 1,image 2 )?

6. If I am doing TC of more than 1 sucker at one go (since I will prepare a min. 500ml or 1lit media), what is the sequence for sterilization ?
Example :- If I have 10 suckers, do I need 10 different bottles of bleach and 10 different bottles of sterile water ?
Or should I put all 10 into one large container containing bleach and another containg sterile water ?

7. Which part of the sterilization process can be done outside the hood and which part inside the hood ?

8. What should be the ideal / average size of explant before dividing into two (after step 6, image 6)?


I am not clear regarding the above. Clarifications on the above doubts would be of great help.

Thanks in advance.

Regards,
Amit
hi
1. suckers? you mean corm ? or plantlets?
2. we can keep corm as long as its still good about aweek
3. keep it at temperature room and dry
4. no yet
5. about 5-8 cm diameter
6. i have 2 corm in one bootle so more economic
7. stage 1-2 outside hood but mext must do in hood/LAminar
8. about 3 cm
sorry my english not well. hope you succes with your TC
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Old 04-22-2012, 10:05 PM   #14 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

I'm new here, and read everything I could here, but still don't see the actual step by step with explicit instructions and details. is this the place to find it ? or do I need to buy a manual......
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Old 04-22-2012, 10:12 PM   #15 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by fruitguy@cox.net View Post
I'm new here, and read everything I could here, but still don't see the actual step by step with explicit instructions and details. is this the place to find it ? or do I need to buy a manual......
Simply Hydroponics & Organics: Step by Step Video for Tissue Culture


Simply Hydroponics Step by Step Tissue Culture part 1 | Household Hydroponics

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Last edited by sunfish : 04-22-2012 at 10:18 PM.
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Old 04-22-2012, 11:16 PM   #16 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Good videos about plant cutting tissue culture, but not bananas
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Old 04-22-2012, 11:20 PM   #17 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by fruitguy@cox.net View Post
Good videos about plant cutting tissue culture, but not bananas
It's a start
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Old 04-23-2012, 01:12 AM   #18 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

There is a book you can buy, it's called 'plants in a test tube' or something lik that, but again doesn't specifically cover bananas. You need to read the threads and a few scientific papers and kind of go from there. This section is full of excellent instructions on how to tissue culture bananas, you just have to look for it.
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Old 04-23-2012, 06:52 AM   #19 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Tissue culture techniques of Banana

https://docs.google.com/viewer?a=v&q...92RBi_mkW_wHXg

http://www.academicjournals.org/ajb/...%20et%20al.pdf
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Old 04-23-2012, 03:00 PM   #20 (permalink)
 
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Default Re: Step by Step How To Tissue Culturing Bananas

Thanks, great thread!
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