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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 01-24-2010, 08:05 AM   #1 (permalink)
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Default step by step Tissue Culture Pineapple

I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.

step 2. throw out the leaf until the last/youngest leaf

step 3. soak in bleach 10 % for 10 minute

step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.

step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA

step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2

step 7. in 2 next month shoot will growth. very beautiful. isnt it?


regards
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Old 01-24-2010, 08:07 AM   #2 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

wooooooo very interesting novi!!!! i will have to do this some time!
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Old 01-24-2010, 08:56 AM   #3 (permalink)
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Thumbs up Re: step by step Tissue Culture Pineapple

Very cool! Thanks for posting this. I may have to try this now someday.
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Old 01-24-2010, 09:03 AM   #4 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Thank you for posting this Novisyatria!! That is good information.
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Old 01-24-2010, 02:59 PM   #5 (permalink)
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Default Re: step by step Tissue Culture Pineapple

WONDERFUL NOVI!!!

Thank you!!! ~Cheryl
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Old 01-24-2010, 06:29 PM   #6 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Yes very cool, I would love to try it Thanks Novi
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Old 01-24-2010, 07:07 PM   #7 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

awsome, thanks for sharing

What do you do after step 7... Do you cut the growth off at its base and put it in rooting hormone, then soil, Or do you cut off the growth with a little bit of the base left and put it in rooting hormone...

Thanks for helping me
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Old 01-25-2010, 07:24 AM   #8 (permalink)
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Default Re: step by step Tissue Culture Pineapple

blake, i can do both. move the shoots in to rooting media and than in soil. and also can cut the shoot and put in same media for shoot multification. so i can still have shoots stock.
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Old 01-30-2010, 10:55 PM   #9 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Great!!
Thanx 4 sharing pak novi
knowledge is power!!
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Old 02-07-2010, 09:52 AM   #10 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Thanks Novi! This is great. I'm going to try this today!
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Old 02-07-2010, 04:05 PM   #11 (permalink)
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Default Re: step by step Tissue Culture Pineapple

Thank You Novi! I have several varieties that I will be mass propagating very soon. Your pictures have helped me understand the process much better than I did after reading about it. I will also start my tc's using the same media formulations that you prescribe here. Thanks again!
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Old 02-07-2010, 04:59 PM   #12 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Cool!!! It's just like TC'ing Bananas!

I'm working on my pineapple right now. I'll post some picts in a few minutes.

Thanks again Novi!!! This is awesome!
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Old 02-07-2010, 05:40 PM   #13 (permalink)
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Default Re: step by step Tissue Culture Pineapple

Quote:
Originally Posted by NANAMAN View Post
Thank You Novi! I have several varieties that I will be mass propagating very soon. Your pictures have helped me understand the process much better than I did after reading about it. I will also start my tc's using the same media formulations that you prescribe here. Thanks again!
Quote:
Originally Posted by Frankco View Post
Cool!!! It's just like TC'ing Bananas!

I'm working on my pineapple right now. I'll post some picts in a few minutes.

Thanks again Novi!!! This is awesome!
your welcome and post your progrees soon ok
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Old 02-07-2010, 06:08 PM   #14 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Okay, I'm done cutting the pineapple up. And to think there is a ham in the freezer


I had this growing in a pot outside. I cut the top off of a pineapple and stuck it ontop of of some dirt in the pot. The frost got to it. I thought it was a goner. The outer leaves died but the inner leave survived. Here is the sad looking pineapple. At least it gave me 10 explants!

Sad Pineapple

Cleaning of the dirt

There's something alive under all of that

Hacking away at it.

It tastes like chicken

Into the disinfecting solution

10 Explants




Thanks Novi!!!!!
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Old 02-07-2010, 07:56 PM   #15 (permalink)
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Default Re: step by step Tissue Culture Pineapple

cool pic Frank. good luck. and post after the explantgrowth ok
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Old 02-07-2010, 08:07 PM   #16 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Hi Novi,

Will do. That was fun! Now to see if they grow

Like you said, it is a thing of beauty to see plants grow from little pieces.

Now I'm going try caladiums. Your pictures of them look fantastic.
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Old 02-07-2010, 11:01 PM   #17 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

This is a great thread!.....keep up the good work guys
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Old 03-01-2010, 05:54 PM   #18 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Sorry everybody. Im still out at the countryside: The Great Savannah!
Ill post some pics next week. Great job Novi! Now Im in a rush to get home ( thats where I have my products ) Thanks a lot.
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Old 07-27-2010, 01:27 PM   #19 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

I wanna do tissue culture of pineapples and was wondering were to buy the little jars and from what I could find the media that novi refers to is different kind of hormones.
Please help me in successful tissue culture
thanks Brian
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Old 07-27-2010, 03:19 PM   #20 (permalink)
 
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Default Re: step by step Tissue Culture Pineapple

Hi Brian,

Canning jars work well.

As for the media and plant growth regulators, you can get them from Carol at Home Tissue Culture* Group

I think you will find her shipping charges much lower than the TC supply houses. You will find her email address on the site if you have any questions.

Frank
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