step by step Tissue Culture Pineapple
 

I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.

step 2. throw out the leaf until the last/youngest leaf

step 3. soak in bleach 10 % for 10 minute

step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.

step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA

step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2

step 7. in 2 next month shoot will growth. very beautiful. isnt it?


regards

Re: step by step Tissue Culture Pineapple
 

wooooooo very interesting novi!!!! i will have to do this some time!

Re: step by step Tissue Culture Pineapple
 

Very cool! Thanks for posting this. I may have to try this now someday.

Re: step by step Tissue Culture Pineapple
 

Thank you for posting this Novisyatria!! That is good information. :2239:

Re: step by step Tissue Culture Pineapple
 

WONDERFUL NOVI!!!

Thank you!!! :D ~Cheryl

Re: step by step Tissue Culture Pineapple
 

Yes very cool, I would love to try it Thanks Novi

Re: step by step Tissue Culture Pineapple
 

awsome, thanks for sharing

What do you do after step 7... Do you cut the growth off at its base and put it in rooting hormone, then soil, Or do you cut off the growth with a little bit of the base left and put it in rooting hormone...

Thanks for helping me :D

Re: step by step Tissue Culture Pineapple
 

blake, i can do both. move the shoots in to rooting media and than in soil. and also can cut the shoot and put in same media for shoot multification. so i can still have shoots stock.

Re: step by step Tissue Culture Pineapple
 

Great!!
Thanx 4 sharing pak novi :)
knowledge is power!!

Re: step by step Tissue Culture Pineapple
 

Thanks Novi! This is great. I'm going to try this today!

Re: step by step Tissue Culture Pineapple
 

Thank You Novi! I have several varieties that I will be mass propagating very soon. Your pictures have helped me understand the process much better than I did after reading about it. I will also start my tc's using the same media formulations that you prescribe here. Thanks again!

Re: step by step Tissue Culture Pineapple
 

Cool!!! It's just like TC'ing Bananas!

I'm working on my pineapple right now. I'll post some picts in a few minutes.

Thanks again Novi!!! This is awesome!

Re: step by step Tissue Culture Pineapple
 

your welcome and post your progrees soon ok

Re: step by step Tissue Culture Pineapple
 

Okay, I'm done cutting the pineapple up. And to think there is a ham in the freezer :nanadrink:


I had this growing in a pot outside. I cut the top off of a pineapple and stuck it ontop of of some dirt in the pot. The frost got to it. I thought it was a goner. The outer leaves died but the inner leave survived. Here is the sad looking pineapple. At least it gave me 10 explants! :woohoonaner:

Sad Pineapple

Cleaning of the dirt

There's something alive under all of that:goteam:

Hacking away at it.

It tastes like chicken:ha:

Into the disinfecting solution

10 Explants:woohoonaner:


:drum:

Thanks Novi!!!!!

Re: step by step Tissue Culture Pineapple
 

cool pic Frank. good luck. and post after the explantgrowth ok

Re: step by step Tissue Culture Pineapple
 

Hi Novi,

Will do. That was fun! Now to see if they grow :drum:

Like you said, it is a thing of beauty to see plants grow from little pieces.

Now I'm going try caladiums. Your pictures of them look fantastic. :goteam:

Re: step by step Tissue Culture Pineapple
 

This is a great thread!.....keep up the good work guys:bananas_b

Re: step by step Tissue Culture Pineapple
 

Sorry everybody. I´m still out at the countryside: The Great Savannah!
I´ll post some pics next week. Great job Novi! Now I´m in a rush to get home ( that´s where I have my products ) Thanks a lot.

Re: step by step Tissue Culture Pineapple
 

I wanna do tissue culture of pineapples and was wondering were to buy the little jars and from what I could find the media that novi refers to is different kind of hormones.
Please help me in successful tissue culture
thanks Brian

Re: step by step Tissue Culture Pineapple
 

Hi Brian,

Canning jars work well.

As for the media and plant growth regulators, you can get them from Carol at Home Tissue Culture* Group

I think you will find her shipping charges much lower than the TC supply houses. You will find her email address on the site if you have any questions.

Frank