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Old 01-24-2010, 08:05 AM   #1 (permalink)
novisyatria
Tissue Culturist
 
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Location: Jakarta, Indonesia
Name: Novi Syatria
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Default step by step Tissue Culture Pineapple

I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.

step 2. throw out the leaf until the last/youngest leaf

step 3. soak in bleach 10 % for 10 minute

step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.

step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA

step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2

step 7. in 2 next month shoot will growth. very beautiful. isnt it?


regards
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Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
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