I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.
step 2. throw out the leaf until the last/youngest leaf
step 3. soak in bleach 10 % for 10 minute
step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.
step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA
step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2
step 7. in 2 next month shoot will growth. very beautiful. isnt it?
regards