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rohsen · 11-11-2008, 04:14 PM

Yet another way to micropropagate plants, this is all about activating the plants own ability to generate all necesary PGRs and let it grow in sugar-hormone free media under high levels of Co2 strong illumination and a low relative humidity. no sugar is needed because the plant is stimulated by the raised illumination to photosynthezise and use the Co2 as a carbon source. by eliminating sugar you eliminate most contamination problems. the explants will be single nodes with 1 or 2 leaves attached to allow photosythesis. All this happens in a closed large vessel with hundreds of plants in regular trays using fibrous substrates such as cellulose vermiculite mixtures, the sytems stimulates root growth and plants need not aclimatize!! they come out fully hardened and rooted !! the most important factor is the C02 level, values of 1500 to 3000 ppm are discussed, the vessel needs to be force ventilated to keep these levels at a constant.

it is a very elegant way of producing plantlets, by lowering the humidity the plant will "sweat" away moisture and suck up nutrients, there is no longer a need to discover the perfect medium nor do you need to invest in PGRs or sugar, it will fix Co2 into plant matter along the way grow roots and be hardened by the forced ventilation, all you need to do is ventilate efficiently keep Co2 at the right level and pump up the PPF (light)

Bananas do great in this system pioneered by a japanese scientist named Toyoki Kozai.

I have very detailed discriptions on how to build these vessels, have sofar had no use for such a system but would wilingly suply info for people interested as long as experiences are shared. i will attach a few publications.
one on coffee and banana and one more general explaining it in more detail

regards
Dave