Composition of the TC media

YAKUZA · 09-04-2011, 07:39 AM

hi tc scientists.

what media you use for musa?
Composition of the media.

Greetings Yakuza

jmoore · 09-04-2011, 08:24 AM

Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

YAKUZA · 09-04-2011, 08:37 AM

Quote:

Originally Posted by jmoore

Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

interesting. you do not use acid?
For example, NAA or IAA

jmoore · 09-04-2011, 10:28 AM

NAA is for rooting and IAA can be added, but apparently it's not heat stable so will break down when you autoclave. I read that on another post here, I don't think it's essential and only really used for making callus, which you wont be doing.

YAKUZA · 09-04-2011, 12:54 PM

are there members who use a different composition?

kentiopsis · 09-04-2011, 05:46 PM

I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.

Aloha from Honolulu,
Ken

Default Re: Composition of the media.
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

YAKUZA · 09-05-2011, 12:14 PM

Quote:

Originally Posted by kentiopsis

I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.

Aloha from Honolulu,
Ken

Default Re: Composition of the media.
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

It is a medium for tissue culture
In a nutshell.
vitamins, growth hormones / regulators, sugar, anti mold
the agar makes everything from liquid to solid.
such as gelatin

kentiopsis · 09-05-2011, 12:37 PM

Thank you, Yakuza. I'm familiar with tissue culture; it's widely used for orchids and seems like a stone natural for bananas. I plan to retire soon to Thailand and plan to devote serious effort to interesting banana cultivars. I hope I can connect with some Bananas.org members over there.

Aloha,
Ken

09-04-2011, 07:39 AM	#1 (permalink)
YAKUZA YAKUZA's Photo Gallery Location: Belgium,Morkhoven Name: Yakuza Join Date: Oct 2008 Posts: 178 BananaBucks : 96,551 Feedback: 0 / 0% Said "Thanks" 3 Times Was Thanked 45 Times in 21 Posts Said "Welcome to Bananas" 0 Times	Composition of the TC media hi tc scientists. what media you use for musa? Composition of the media. Greetings Yakuza __________________ exploring beer brewing

09-04-2011, 08:24 AM	#2 (permalink)
jmoore jmoore 's Photo Gallery Location: Lowestoft, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 766 BananaBucks : 154,431 Feedback: 4 / 100% Said "Thanks" 192 Times Was Thanked 799 Times in 373 Posts Said "Welcome to Bananas" 71 Times	Re: Composition of the media. Try this: To make 1 litre of media, use distilled water. 2.1 grams MS medium 30 grams sugar 5 mg of BAP (5ml of stock 1000 mg / L solution) 8 grams of agar 1 ml PPM You can add vitamins and coconut water and Indole Acetic acid, but start with this.

09-04-2011, 10:28 AM	#4 (permalink)
jmoore jmoore 's Photo Gallery Location: Lowestoft, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 766 BananaBucks : 154,431 Feedback: 4 / 100% Said "Thanks" 192 Times Was Thanked 799 Times in 373 Posts Said "Welcome to Bananas" 71 Times	Re: Composition of the media. NAA is for rooting and IAA can be added, but apparently it's not heat stable so will break down when you autoclave. I read that on another post here, I don't think it's essential and only really used for making callus, which you wont be doing.

09-04-2011, 12:54 PM	#5 (permalink)
YAKUZA YAKUZA's Photo Gallery Location: Belgium,Morkhoven Name: Yakuza Join Date: Oct 2008 Posts: 178 BananaBucks : 96,551 Feedback: 0 / 0% Said "Thanks" 3 Times Was Thanked 45 Times in 21 Posts Said "Welcome to Bananas" 0 Times	Re: Composition of the media. are there members who use a different composition? __________________ exploring beer brewing

09-04-2011, 05:46 PM	#6 (permalink)
kentiopsis kentiopsis Location: Khonkaen, Thailand, an agricultural and horticultural center in NE Thailand Find me on the map! Zone: Unknown, but tropical Name: kentiopsis Join Date: Jul 2011 Posts: 80 BananaBucks : 39,679 Feedback: 0 / 0% Said "Thanks" 27 Times Was Thanked 103 Times in 47 Posts Said "Welcome to Bananas" 13 Times	Re: Composition of the TC media I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this. Aloha from Honolulu, Ken Default Re: Composition of the media. Try this: To make 1 litre of media, use distilled water. 2.1 grams MS medium 30 grams sugar 5 mg of BAP (5ml of stock 1000 mg / L solution) 8 grams of agar 1 ml PPM You can add vitamins and coconut water and Indole Acetic acid, but start with this.

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09-05-2011, 12:37 PM	#8 (permalink)
kentiopsis kentiopsis Location: Khonkaen, Thailand, an agricultural and horticultural center in NE Thailand Find me on the map! Zone: Unknown, but tropical Name: kentiopsis Join Date: Jul 2011 Posts: 80 BananaBucks : 39,679 Feedback: 0 / 0% Said "Thanks" 27 Times Was Thanked 103 Times in 47 Posts Said "Welcome to Bananas" 13 Times	Re: Composition of the TC media Thank you, Yakuza. I'm familiar with tissue culture; it's widely used for orchids and seems like a stone natural for bananas. I plan to retire soon to Thailand and plan to devote serious effort to interesting banana cultivars. I hope I can connect with some Bananas.org members over there. Aloha, Ken

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