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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 09-04-2011, 07:39 AM   #1 (permalink)
 
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Default Composition of the TC media

hi tc scientists.

what media you use for musa?
Composition of the media.

Greetings Yakuza
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Old 09-04-2011, 08:24 AM   #2 (permalink)
 
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Default Re: Composition of the media.

Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.
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Old 09-04-2011, 08:37 AM   #3 (permalink)
 
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Default Re: Composition of the media.

Quote:
Originally Posted by jmoore View Post
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

interesting. you do not use acid?
For example, NAA or IAA
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Old 09-04-2011, 10:28 AM   #4 (permalink)
 
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Default Re: Composition of the media.

NAA is for rooting and IAA can be added, but apparently it's not heat stable so will break down when you autoclave. I read that on another post here, I don't think it's essential and only really used for making callus, which you wont be doing.
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Old 09-04-2011, 12:54 PM   #5 (permalink)
 
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Default Re: Composition of the media.

are there members who use a different composition?
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Old 09-04-2011, 05:46 PM   #6 (permalink)
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Default Re: Composition of the TC media

I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.

Aloha from Honolulu,
Ken

Default Re: Composition of the media.
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.
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Old 09-05-2011, 12:14 PM   #7 (permalink)
 
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Default Re: Composition of the TC media

Quote:
Originally Posted by kentiopsis View Post
I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.

Aloha from Honolulu,
Ken

Default Re: Composition of the media.
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

It is a medium for tissue culture
In a nutshell.
vitamins, growth hormones / regulators, sugar, anti mold
the agar makes everything from liquid to solid.
such as gelatin
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Last edited by YAKUZA : 09-05-2011 at 12:16 PM.
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Old 09-05-2011, 12:37 PM   #8 (permalink)
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Default Re: Composition of the TC media

Thank you, Yakuza. I'm familiar with tissue culture; it's widely used for orchids and seems like a stone natural for bananas. I plan to retire soon to Thailand and plan to devote serious effort to interesting banana cultivars. I hope I can connect with some Bananas.org members over there.

Aloha,
Ken
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