Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
The Facebook Platform
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 10-30-2009, 12:12 AM   #1 (permalink)
 
Location: aust
Join Date: Oct 2009
Posts: 10
BananaBucks : 5,700
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 9 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default media

hi all ,is it best to to keep media a little on the soft side than to firm ?also with initiation does it if you use less 1/2 strenght m/s in media?
landzt is offline   Reply With Quote Send A Private Message To landzt

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 10-30-2009, 03:55 AM   #2 (permalink)
Moderator

 
Gabe15's Avatar
 
Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,674
BananaBucks : 13,218,555
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was Thanked 7,249 Times in 1,958 Posts
Said "Welcome to Bananas" 8 Times
Default Re: media

I have found it doesn't matter too much if its soft or firm, some systems don't use any gelling agent at all.

I would use at least 1/2 strength for standard micropropagation, though you may find that some varieties will be slow and not grow well. These will sometimes like full strength MS better, but I have not ever needed less than 1/2 strength.
__________________
Growing bananas in Colorado, Hawaii and Washington since 2004.
Gabe15 is online now   Reply With Quote Send A Private Message To Gabe15
Old 11-02-2009, 04:41 AM   #3 (permalink)
 
Join Date: Nov 2009
Posts: 2
BananaBucks : 330
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 0 Times in 0 Posts
Said "Welcome to Bananas" 0 Times
Default Re: media

Hello everyone. I have tried tissue culturing bananas using MS + 5 ppm BAP but the results are not good. The plants showed blackening and contaminated.

What shall I do? Are there any modifications to the MS that work good for bananas?

Please help. Thank you.

Cheers,

Faisar
Faisar Mamacotao is offline   Reply With Quote Send A Private Message To Faisar Mamacotao
Old 11-02-2009, 09:20 AM   #4 (permalink)
 
jmoore's Avatar
 
Location: Lowestoft, UK
Zone: 8a
Name: James
Join Date: Aug 2008
Posts: 752
BananaBucks : 79,213
Feedback: 3 / 100%
Said "Thanks" 190 Times
Was Thanked 770 Times in 358 Posts
Said "Welcome to Bananas" 70 Times
Default Re: media

There is a broad spectrum fungicide and biocide on the market called 'ppm' which stands for plant preservation mixture, I think. The concentration is 1ml per litre of media. This may help, I'm using it at the moment, bit early to tell how effective it is yet.

Did you sterilise the explant in bleach before putting it into the media?

How good is your autoclave? Are all of your intruments sterilised prior to use?

The media you're using sounds OK, I think.
jmoore is offline   Reply With Quote Send A Private Message To jmoore
Old 11-04-2009, 11:24 PM   #5 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 479
BananaBucks : 320,391
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 685 Times in 203 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: media

I also use PPM and the results is efective. i add 0,5 ml PPm in to 1 liter MS SOlution. we can add PPM 0,5 -2 PPM (based on manual instruction) but based on my experience 0,5 PPM is enough
regards
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Sponsors

Old 11-05-2009, 05:44 AM   #6 (permalink)
 
Join Date: Nov 2009
Posts: 2
BananaBucks : 330
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 0 Times in 0 Posts
Said "Welcome to Bananas" 0 Times
Default Re: media

Thank you sir for that reply. Though I haven't used the ppm yet, so far contamination has been minimal, using bleach to sterilize the explants. But still, the explants are not growing well.

Since MS is a generic formula, which components of the MS should I vary?

Thank you.
Faisar Mamacotao is offline   Reply With Quote Send A Private Message To Faisar Mamacotao
Old 11-05-2009, 05:54 AM   #7 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 479
BananaBucks : 320,391
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 685 Times in 203 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: media

if explant not growing maybe bleach is too hard or you soak too long so explant not good.
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Old 11-06-2009, 11:59 PM   #8 (permalink)
 
Location: aust
Join Date: Oct 2009
Posts: 10
BananaBucks : 5,700
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 9 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Bananas Brindando Re: media

Hello ,and thank you for the replys to thread i have learned a little more and have a few things to try .Do many people us PPM ? and is it only used in initiation or if a problem a rises.
landzt is offline   Reply With Quote Send A Private Message To landzt
Old 11-07-2009, 01:05 AM   #9 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 479
BananaBucks : 320,391
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 685 Times in 203 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: media

i use PPM for all culture i have, but for initiation aybe more efectice with 1 ml/liter. for subculture 0,5 ml/lt is enough
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Old 11-23-2009, 09:30 AM   #10 (permalink)
 
Location: aust
Join Date: Oct 2009
Posts: 10
BananaBucks : 5,700
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 9 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: media

I have just recieved the PPM ,i have read were it is possible to sterilize media with ppm in ,but also read you can mix ppm in liquid sterilize it and add it to as a cover -like another lay in laminar cabinet i hope i have described it right ,is it best in media and only use liquid on light contamination were ppm is not in media?thanks
landzt is offline   Reply With Quote Send A Private Message To landzt
Old 11-23-2009, 07:17 PM   #11 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 479
BananaBucks : 320,391
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 685 Times in 203 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: media

landt
sorry i dont full undrstand what do you mean. i add PPM to media. and never add to laminar. do you mean spraing IT to laminar? based on manual from plant cell biotechnology, i dont fine some information about sterilize laminar with PPM. its only used as broad spectrum fungal and bacterial and added in media.
regards
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Old 11-24-2009, 02:43 PM   #12 (permalink)
 
Location: cotswolds UK
Name: stevethelizard
Join Date: Apr 2009
Posts: 3
BananaBucks : 3,473
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 2 Times in 1 Post
Said "Welcome to Bananas" 0 Times
Default Re: media

Carol at the home tissue culture group uses a liquid overlay on top of agar media i have never tried but it seems a good idea
stevethelizard is offline   Reply With Quote Send A Private Message To stevethelizard
Old 11-24-2009, 07:20 PM   #13 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 479
BananaBucks : 320,391
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 685 Times in 203 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: media

ohh i got what lantd mean now. maybe its good idea but i think we have to prepare the media 2 times. first wait until media/agar is cold. second we add PPM liquid. thats mean PPm solution better if in steril condition. so we have to filtering firts with syringe filter and when we add PPM must in laminar air flow. give it to media in bottle one by one. i think its needs more time. its oke if we just make a little media but not eficient if a lot media. i make 100-200 bottle/day and if i do like carol maybe not efficient. based on my experience add PPM to media directly when we make media is good enough.
regards
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Reply   Email this Page Email this Page






Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Questions about media Java16 Tissue Culturing & Other Propagation Techniques of Banana Plants 2 10-11-2009 06:23 AM
media composition for culturing banana (poovan) surmomu Member Introductions 4 09-10-2009 07:29 PM
germinating media martin Banana Seed Germination Forum 1 03-01-2009 09:23 AM
Tip: Cheapest planting media JoeReal Main Banana Discussion 3 07-15-2005 08:47 PM


All times are GMT -5. The time now is 05:09 PM.





Follow us:
Twitter YouTube

All content © Bananas.org & the respective author.