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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 10-10-2009, 12:33 PM   #1 (permalink)
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Default Questions about media

Hi everyone,
I am a horticulture major at Penn State and would like to start experimenting with banana tissue culture. I have a few questions. First, when tissue culturing bananas, do you tissue culture the explants in different media for different stages (for example is it necessary to use one media for shoots and then transfer them into another media for roots?) or can you simply keep them in the same media for shoot and root growth? Also, does anyone have an easy to follow recipe for making media? Thanks
-Dave
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Old 10-10-2009, 06:32 PM   #2 (permalink)
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Default Re: Questions about media

Dear Dave
For bananas, just using same media MS + BAP 3-5 mg/l. banans dont need auxin because can make rooting wihtout auxin. you can ask me about TC bananas in this thread or PM me.

regards from jakarta
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Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
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Old 10-11-2009, 06:23 AM   #3 (permalink)
 
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Default Re: Questions about media

http___www.geocities.com_k2benson_TCoverview_TCban_TCban.pdf

This has a good recipe for media, I'll be trying this method in a couple of weeks in fact.
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