Re: Musa Velutina Experiment
i did some experimenting with musa velutina seeds myself according to attached publication. The first time I sterilized the 3 month old M velutina seeds for 2 minutes in 70% ethanol, then for 45 minutes in a 10% household bleach solution. followed by 3 rinses in sterile water. Then i cracked the seeds and took out the embryos with the tip of a scalpel under a stereomicroscope.
and placed them in petridishes containing 1/2 ms medium containing 0.1 milimole of gibberelic acid (34.6 microliter per liter)
the result: severe contamination....
so the 2nd time 4 minutes in ethanol and 20% bleach for 45min, this worked fine nice little plantlets after about 2 weeks, these i used for further micropropagation experiments with varying results. for me this was a very good method to get sterile starting material.
i have attached the article.
also it was found that musa velutina seeds lose viability after about 6 months.
cheers
Dave
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