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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 10-04-2008, 07:40 PM   #1 (permalink)
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Default My home TC lab is open, Finally!

Just finished setting up a few things and starting my first culture today, an embryo rescue is first on my agenda.

The flow hood is in my photo gallery along with some pics of the recently received starts.

Will continue to post progress reports as more gets going.
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Old 10-04-2008, 07:43 PM   #2 (permalink)
 
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Default Re: My home TC lab is open, Finally!

nice man hey did u ever send the seeds cause i never got anything...if not well its cool thanks anyway
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Old 10-04-2008, 07:53 PM   #3 (permalink)
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Default Re: My home TC lab is open, Finally!

Congratulations, Scot!!!!
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Old 10-04-2008, 08:19 PM   #4 (permalink)
 
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Default Re: My home TC lab is open, Finally!

Very cool. Looking forward to watching your progress as I slowly accumulate my lab.
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Old 10-04-2008, 08:28 PM   #5 (permalink)
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Default Re: My home TC lab is open, Finally!

Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.
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Old 10-04-2008, 08:33 PM   #6 (permalink)
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Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by Gabe15 View Post
Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.
Good advice, Gabe. Will do. I set up some petri dishes with M&S medium in them just for that purpose since reading your post.
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Old 10-04-2008, 10:01 PM   #7 (permalink)
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Default Re: My home TC lab is open, Finally!

OMG, I just roasted some petri dishes. Check out my gallery. Well now I know.
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Old 10-05-2008, 12:30 AM   #8 (permalink)
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Default Re: My home TC lab is open, Finally!

LOL, I saw those at the top of the forum and was like "Uh oh"

Your set up looks great, I wish I had the time to try my hand at TC's. For now I'll have to settle for the pups.
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Old 10-05-2008, 06:13 PM   #9 (permalink)
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Default Re: My home TC lab is open, Finally!

Gabe, the agar is clear so far, so I am hoping to have aseptic conditions in the hood. Wish me luck!!! I just went ahead and did 5 seeds for embryo rescue - man those little buggers are hard to hold and see. Hopefully the embryos weren't mangled too much. We will have to see if anything grows besides contamination. I tried to be sooooo careful about that, but it's a crap shoot.
If they get something going I will post some pics. I placed each GA7 jar into a zip-loc baggie afterwards (done in the hood) so I am hoping to avoid contamination there, too. I hope at least one of them is a good rescue, but they are so difficult to see, much less to slice the seed. The first one, the scalpel blade broke and the seed went flying. I found it, resterilized everything and tried again, but the embryo looked rotten. It was all brown - perhaps oxidized from leaving the seeds in 10% bleach solution overnight! Oops!
How do you hold onto the seeds and cut them? I found it very difficult using forceps and a surgical scalpel. Is there another, easier way to do this? When you do ER, do you use a macroscope to see better? I was thinking of doing that, but after the difficulty cutting the seed, there is no way I can imagine doing this on a macroscope stage without knocking it over. Maybe one of the reverse viewing types with the stage as part of the base????
Anyway, the first cultures are going now. I have a Saba corm that's not doing anything after 3-4 weeks, so I might grab it to TC it. It has one more week to show me signs of life, or it's going under the knife!!!
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Old 10-06-2008, 09:04 AM   #10 (permalink)
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Default Re: My home TC lab is open, Finally!

congrats scot! im not that ambitious yet.....i want to try growing from seed first but i have a lot more to learn before i even start that. congrats again!!!!!
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Old 10-06-2008, 11:12 AM   #11 (permalink)
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Default Re: My home TC lab is open, Finally!

Way to go Scot! This is something I have always been dreaming about, now that you are onto to it, its gonna be 'look n learn' from your updates. All the best mate.
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Old 10-06-2008, 11:42 AM   #12 (permalink)
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Default Re: My home TC lab is open, Finally!

Yea, way to go! Sounds awesome!
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Old 10-06-2008, 12:48 PM   #13 (permalink)
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Default Re: My home TC lab is open, Finally!

Good luck, Scot! Sounds like you're having fun now!
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Old 10-06-2008, 01:14 PM   #14 (permalink)
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Sounds really cool, once you have it down the sky's the limit I guess. The petri dishes look like Frankenstein got to them though!
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Old 10-10-2008, 03:09 AM   #15 (permalink)
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Default Re: My home TC lab is open, Finally!

Perhaps you had seen this post awhile back, but this was my first couple attempts at embryo rescue, I also briefly detail some tips on opening the seeds.

Germination the easy way
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Old 10-10-2008, 03:10 AM   #16 (permalink)
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Default Re: My home TC lab is open, Finally!

Yes, I saw and learned from it. Thank you Gabe.
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Old 10-21-2008, 01:50 AM   #17 (permalink)
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Default Re: My home TC lab is open, Finally!

Just put Manzano and another cold hardy banana into culture. The Manzano was last week, the cold hardy banana this evening. The one this evening was my best apical meristem dissection yet. It was textbook, except the media was still a bit soft when I put it in, so it sunk in a bit deeper than I wanted it, but that means more exposure to the media.
I was going to TC the Saba corm, but as I was lifting it out of the pot, I noticed that roots were growing like crazy and pups were forming, so I left it alone.
I will have some pictures tomorrow in my gallery. My camera isn't that great, so the resolution is so so. But a couple turned out ok.
The Manzano explant is elongating and no signs of contamination.
As for the Embryo rescue, despite being on the wrong media, it looks like there are some changes going on with one of the cultures, so I am optimistic that at least one will take. (cross your fingers!)
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Old 10-21-2008, 11:57 AM   #18 (permalink)
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Default Re: My home TC lab is open, Finally!

Good luck, Scot. Sounds like it's going well thus far.
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Old 10-21-2008, 01:25 PM   #19 (permalink)
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Congrats!!
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Old 10-21-2008, 04:48 PM   #20 (permalink)
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Default Re: My home TC lab is open, Finally!

As promised, here is a picture of the apical meristem, ready to go into the media. To give you some scale, the lid it is sitting in is for a baby food jar.





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