![]() |
Welcome to the Bananas.org forums. You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today! If you have any problems with the registration process or your account login, please contact us. |
|
Register | Photo Gallery | Classifieds | Wiki | Chat | Map | Search | Today's Posts | Mark Forums Read |
Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
![]() |
|
![]() |
The most chatters online in one day was 17, 09-06-2009. No one is currently using the chat. |
![]() ![]() |
|
LinkBack | Thread Tools |
![]() |
#1 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() ![]() The flow hood is in my photo gallery along with some pics of the recently received starts. Will continue to post progress reports as more gets going. Last edited by Chironex : 10-04-2008 at 09:59 PM. |
![]() |
![]() ![]() |
Said thanks: |
Sponsors |
![]() |
#2 (permalink) |
Join Date: Sep 2007
Posts: 837
BananaBucks
: 8,353
Feedback: 19 / 100%
Said "Thanks" 228 Times
Was
Thanked 308 Times in 165 Posts
Said "Welcome to Bananas" 100 Times
|
![]() nice man hey did u ever send the seeds cause i never got anything...if not well its cool thanks anyway
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#3 (permalink) |
Senior Member
![]() Location: Seattle, WA
Zone: 8-9
Name: Chong
Join Date: Nov 2006
Posts: 1,845
BananaBucks
: 89,209
Feedback: 6 / 100%
Said "Thanks" 1,306 Times
Was
Thanked 1,696 Times in 756 Posts
Said "Welcome to Bananas" 991 Times
|
![]() Congratulations, Scot!!!!
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#4 (permalink) |
Location: Boca Raton, Florida
Zone: 10
Name: Tom
Join Date: Jul 2008
Posts: 92
BananaBucks
: 6,657
Feedback: 0 / 0%
Said "Thanks" 10 Times
Was
Thanked 40 Times in 30 Posts
Said "Welcome to Bananas" 30 Times
|
![]() Very cool. Looking forward to watching your progress as I slowly accumulate my lab.
![]() |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#5 (permalink) |
Moderator
![]() ![]() Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,875
BananaBucks
: 13,290,837
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was
Thanked 8,117 Times in 2,180 Posts
Said "Welcome to Bananas" 8 Times
|
![]() Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.
__________________
Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties. |
![]() |
![]() ![]() |
Said thanks: |
Sponsors |
![]() |
#6 (permalink) | |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() Quote:
|
|
![]() |
![]() ![]() |
![]() |
#7 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() OMG, I just roasted some petri dishes. Check out my gallery. Well now I know.
|
![]() |
![]() ![]() |
![]() |
#8 (permalink) |
KeoniKale
Location: Lexington, SC
Zone: 8b
Name: John
Join Date: Sep 2008
Posts: 324
BananaBucks
: 62,571
Feedback: 2 / 100%
Said "Thanks" 68 Times
Was
Thanked 126 Times in 73 Posts
Said "Welcome to Bananas" 15 Times
|
![]() LOL, I saw those at the top of the forum and was like "Uh oh"
Your set up looks great, I wish I had the time to try my hand at TC's. For now I'll have to settle for the pups.
__________________
Malama 'Aina Normal Jan. Low: 34°F, Normal Jul High: 92°F Coldest: 16-20°F, Warmest: 98-103°F Annual Precip: 48 inches |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#9 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() Gabe, the agar is clear so far, so I am hoping to have aseptic conditions in the hood. Wish me luck!!! I just went ahead and did 5 seeds for embryo rescue - man those little buggers are hard to hold and see. Hopefully the embryos weren't mangled too much. We will have to see if anything grows besides contamination. I tried to be sooooo careful about that, but it's a crap shoot.
If they get something going I will post some pics. I placed each GA7 jar into a zip-loc baggie afterwards (done in the hood) so I am hoping to avoid contamination there, too. I hope at least one of them is a good rescue, but they are so difficult to see, much less to slice the seed. The first one, the scalpel blade broke and the seed went flying. I found it, resterilized everything and tried again, but the embryo looked rotten. It was all brown - perhaps oxidized from leaving the seeds in 10% bleach solution overnight! Oops! How do you hold onto the seeds and cut them? I found it very difficult using forceps and a surgical scalpel. Is there another, easier way to do this? When you do ER, do you use a macroscope to see better? I was thinking of doing that, but after the difficulty cutting the seed, there is no way I can imagine doing this on a macroscope stage without knocking it over. Maybe one of the reverse viewing types with the stage as part of the base???? Anyway, the first cultures are going now. I have a Saba corm that's not doing anything after 3-4 weeks, so I might grab it to TC it. It has one more week to show me signs of life, or it's going under the knife!!! Last edited by Chironex : 10-05-2008 at 06:20 PM. Reason: added |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#10 (permalink) |
banana junkie
Location: north carolina
Zone: 7b
Name: mskitty
Join Date: Nov 2007
Posts: 4,071
BananaBucks
: 170,059
Feedback: 26 / 100%
Said "Thanks" 82 Times
Was
Thanked 890 Times in 617 Posts
Said "Welcome to Bananas" 136 Times
|
![]() congrats scot! im not that ambitious yet.....i want to try growing from seed first but i have a lot more to learn before i even start that. congrats again!!!!!
![]() ![]() ![]() ![]() ![]() |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#11 (permalink) |
Ensete nut
![]() Location: Kuala Lumpur, Malaysia
Name: Tog
Join Date: Jul 2008
Posts: 1,757
BananaBucks
: 397,842
Feedback: 2 / 100%
Said "Thanks" 1,011 Times
Was
Thanked 2,721 Times in 787 Posts
Said "Welcome to Bananas" 204 Times
|
![]() Way to go Scot! This is something I have always been dreaming about, now that you are onto to it, its gonna be 'look n learn' from your updates. All the best mate.
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#12 (permalink) |
Tally-Man
![]() ![]() Location: Davie, Florida
Zone: 10
Name: Jarred
Join Date: Jul 2005
Posts: 5,249
BananaBucks
: 1,720,436
Feedback: 66 / 100%
Said "Thanks" 3,793 Times
Was
Thanked 4,996 Times in 1,339 Posts
Said "Welcome to Bananas" 2,059 Times
|
![]() Yea, way to go! Sounds awesome!
__________________
Apologies in advance if I am slow to reply to your PM. I suggest posting in the forums for support if you need something urgent. |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#13 (permalink) |
Bananaculturist
![]() Location: Houston, TX area
Zone: 9
Name: Brent
Join Date: Jun 2007
Posts: 3,017
BananaBucks
: 193,025
Feedback: 22 / 100%
Said "Thanks" 1,337 Times
Was
Thanked 2,257 Times in 1,175 Posts
Said "Welcome to Bananas" 191 Times
|
![]() Good luck, Scot! Sounds like you're having fun now!
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#14 (permalink) |
Orang Puteh
![]() Location: Morris plains N.J.
Zone: 6a
Name: bob
Join Date: Sep 2008
Posts: 3,527
BananaBucks
: 185,696
Feedback: 12 / 100%
Said "Thanks" 9,531 Times
Was
Thanked 4,052 Times in 1,691 Posts
Said "Welcome to Bananas" 565 Times
|
![]() Sounds really cool, once you have it down the sky's the limit I guess. The petri dishes look like Frankenstein got to them though!
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#15 (permalink) |
Moderator
![]() ![]() Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,875
BananaBucks
: 13,290,837
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was
Thanked 8,117 Times in 2,180 Posts
Said "Welcome to Bananas" 8 Times
|
![]() Perhaps you had seen this post awhile back, but this was my first couple attempts at embryo rescue, I also briefly detail some tips on opening the seeds.
Germination the easy way
__________________
Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties. |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#16 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() Yes, I saw and learned from it. Thank you Gabe.
|
![]() |
![]() ![]() |
Sponsors |
![]() |
#17 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() Just put Manzano and another cold hardy banana into culture. The Manzano was last week, the cold hardy banana this evening. The one this evening was my best apical meristem dissection yet. It was textbook, except the media was still a bit soft when I put it in, so it sunk in a bit deeper than I wanted it, but that means more exposure to the media.
I was going to TC the Saba corm, but as I was lifting it out of the pot, I noticed that roots were growing like crazy and pups were forming, so I left it alone. I will have some pictures tomorrow in my gallery. My camera isn't that great, so the resolution is so so. But a couple turned out ok. The Manzano explant is elongating and no signs of contamination. As for the Embryo rescue, despite being on the wrong media, it looks like there are some changes going on with one of the cultures, so I am optimistic that at least one will take. (cross your fingers!) |
![]() |
![]() ![]() |
![]() |
#18 (permalink) |
Bananaculturist
![]() Location: Houston, TX area
Zone: 9
Name: Brent
Join Date: Jun 2007
Posts: 3,017
BananaBucks
: 193,025
Feedback: 22 / 100%
Said "Thanks" 1,337 Times
Was
Thanked 2,257 Times in 1,175 Posts
Said "Welcome to Bananas" 191 Times
|
![]() Good luck, Scot. Sounds like it's going well thus far.
|
![]() |
![]() ![]() |
Said thanks: |
![]() |
#19 (permalink) |
kimarj
Location: Philadelphia PA
Zone: 6 - 7
Name: Kim
Join Date: Jan 2008
Posts: 66
BananaBucks
: 5,113
Feedback: 0 / 0%
Said "Thanks" 177 Times
Was
Thanked 6 Times in 6 Posts
Said "Welcome to Bananas" 29 Times
|
![]() ![]() ![]() ![]() |
![]() |
![]() ![]() |
Said thanks: |
![]() |
#20 (permalink) |
The causasian Asian!
![]() Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks
: 99,592
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was
Thanked 1,404 Times in 806 Posts
Said "Welcome to Bananas" 609 Times
|
![]() |
![]() |
![]() ![]() |
Said thanks: |
Sponsors |
![]() ![]() |
Currently Active Users Viewing This Thread: 1 (0 members and 1 guests) | |
Thread Tools | |
|