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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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09-05-2020, 09:37 PM | #1 (permalink) |
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Genetics of banana parthenocarpy
Hello! I’m new to the forum and I’m unsure of where to ask this but this sub forum seems closest, especially considering my plan will involve a decent amount of micropropagation.
So I’m a biotech guy/lab tech by trade and I’m gonna do something dumb with banana genetics. My plan is to use somatic fusion to make a hybrid between a short cycle AA type banana like Patupi and M. Velutina which I’ve heard flowers and ripens quickly/have personally seen set fruit and ripen in a local Botanical garden, then make a Triploid if necessary either by crossing the result back to velutina or crossing it with a M. itinerans or a basjoo. The end goal is a cold hardy edible banana I have the tools, tech, and resources (though I’ll need to practice the exact skills) but my main question is: How many copies of the parthenocarpy genes are Necessary for the banana to be able to have edible fruit? |
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09-11-2020, 03:58 PM | #2 (permalink) |
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Re: Genetics of banana parthenocarpy
Only one copy is needed but there are multiple genes. Even with lab skills, I think your plans are overly ambitious and you should look deeper into the genetic relationships between these species you want to work with. For example, AA cultivars and M. velutina can more or less cross ok by conventional methods, but M. itinerans and M. basjoo are close to each other but far from M. acuminata and M. velutina, and are quite incompatible even with pure species, not to mention hybrids or otherwise mixed-genome individuals. Also, expecting to produce a triploid by crossing means you ought to start with a tetraploid and cross to diploids, which means doing chromosome doubling first (not necessarily an easy task, but doable, however confirming your results is a labor intensive process unless you have free access to a flow cytometer). I understand the fun in playing around in the lab, but if you want to start somewhere that has a chance but far less complicated, just try crossing Patupi and M. velutina conventionally by pollination. That’s a serious challenge in it’s own right just horticulturally speaking, but if you can’t manage even that I don’t see how any of your other plans are going to stand a chance or result in anything.
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09-11-2020, 04:43 PM | #3 (permalink) | |
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Re: Genetics of banana parthenocarpy
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09-12-2020, 03:20 PM | #4 (permalink) | |
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Re: Genetics of banana parthenocarpy
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Thank you the info though! It’s good to know the relationship between M. Velutina and Patupi is somewhat close and that only a few genes will be hypothetically necessary for parthenocarpy. Somatic fusion will inherently produce tetraploids with the AAVV genome so making a triploid from it should be relatively easy by backcrossing it to velutina. Somatic fusion’s main draw as a technique is it allows two sexually incompatible species to be “crossed” so there may be merit in just applying the technique with Patupi and basjoo. However, my concerns with that are that basjoo I’ve heard is a relatively long cycle banana which isn’t great and the higher sexual incompatible probably will affect the male fertility making it a genetic dead end. |
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09-13-2020, 01:01 PM | #5 (permalink) | |
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09-13-2020, 03:27 PM | #6 (permalink) | |
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