Gabe15

Only one copy is needed but there are multiple genes. Even with lab skills, I think your plans are overly ambitious and you should look deeper into the genetic relationships between these species you want to work with. For example, AA cultivars and M. velutina can more or less cross ok by conventional methods, but M. itinerans and M. basjoo are close to each other but far from M. acuminata and M. velutina, and are quite incompatible even with pure species, not to mention hybrids or otherwise mixed-genome individuals. Also, expecting to produce a triploid by crossing means you ought to start with a tetraploid and cross to diploids, which means doing chromosome doubling first (not necessarily an easy task, but doable, however confirming your results is a labor intensive process unless you have free access to a flow cytometer). I understand the fun in playing around in the lab, but if you want to start somewhere that has a chance but far less complicated, just try crossing Patupi and M. velutina conventionally by pollination. That’s a serious challenge in it’s own right just horticulturally speaking, but if you can’t manage even that I don’t see how any of your other plans are going to stand a chance or result in anything.

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Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.