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Old 09-05-2011, 12:14 PM   #7 (permalink)
YAKUZA
 
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Default Re: Composition of the TC media

Quote:
Originally Posted by kentiopsis View Post
I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.

Aloha from Honolulu,
Ken

Default Re: Composition of the media.
Try this:

To make 1 litre of media, use distilled water.

2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM

You can add vitamins and coconut water and Indole Acetic acid, but start with this.

It is a medium for tissue culture
In a nutshell.
vitamins, growth hormones / regulators, sugar, anti mold
the agar makes everything from liquid to solid.
such as gelatin
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Last edited by YAKUZA : 09-05-2011 at 12:16 PM.
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