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Old 07-19-2011, 06:47 PM   #8 (permalink)
noksu
 
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Default Re: Need Help ! Surface Sterilization of plant material

Hi,
here i am again

@ jmoore

Quote:
Originally Posted by jmoore View Post
15% is the free chlorine concentration and doesn't refer to the NaClO concentration. Don't use the bleach undiluted it will kill your explant.

You want 10% by volume of whatever bleach you have. so 1000 cm3 requires 900 cm3 of water and 100 cm3 of bleach and so on. It can be the cheapest you can buy.

I hope this makes sense

ok, for an example :
I take Commercial household bleach which contains 5% sodium hypochlorite.
I have to mix 1 part bleach with 9 parts water and i get a 10 % solution.
so far so good.....but it means the solution contains only
0,5 % sodium hypochlorite. ?
Is this right ?
And that will it be enough ???
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