Bananas.org - View Single Post - Need Help ! Surface Sterilization of plant material

ImperialExotics · 07-17-2011, 02:09 AM

To expand a little on what Gabe said........

Sterilize your initial explant. After it is fully sterilized remove another complete layer of that explant, and go through the process of sterilization AGAIN.

Then remove a second complete layer of the explant and it is ready for innoculation.

I also read a paper suggesting treating the tissue for an 18 hour soak in Bavistin to kill fungus and fungal spores, prior to the sterilization and trimming procedure.

07-17-2011, 02:09 AM	#6 (permalink)
ImperialExotics Join Date: Apr 2011 Posts: 53 BananaBucks : 33,631 Feedback: 0 / 0% Said "Thanks" 2 Times Was Thanked 65 Times in 34 Posts Said "Welcome to Bananas" 2 Times	Re: Need Help ! Surface Sterilization of plant material To expand a little on what Gabe said........ Sterilize your initial explant. After it is fully sterilized remove another complete layer of that explant, and go through the process of sterilization AGAIN. Then remove a second complete layer of the explant and it is ready for innoculation. I also read a paper suggesting treating the tissue for an 18 hour soak in Bavistin to kill fungus and fungal spores, prior to the sterilization and trimming procedure.