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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 01-18-2013, 05:11 PM   #1 (permalink)
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Default What part to use?

Hello everyone. while i'm not even close to be able to tissue culture my banana yet, I've got a few questions i might as well get cleared up for when that time comes . Most of them i could probably find the answers for somewhere else, but my biggest question is WHAT PART TO USE?!? I have seen stuff about using meristem from the corm, but some sites have talked about other areas, like the flower. The info i was looking at recently said:
Quote:
Shoot cultures of banana start conventionally from any plant part that contains a shoot meristem, i.e. the parental pseudostem, small suckers, peepers and lateral buds . The apex of the inflorescence and axillary flower buds are also suitable explants for tissue culture initiation.
What is that supposed to mean?
Also, on a similar tangent, I have seem some stuff about nicking and dividing corms. What is that supposed to mean and how do I do that?
Can anyone help me out here?
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Old 01-18-2013, 05:43 PM   #2 (permalink)
 
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Default Re: What part to use?

Just a quick Google and I found this informative slideshow that details the tissue culture process. Hope it helps out!

Tissue culture techniques of Banana
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Old 01-18-2013, 07:00 PM   #3 (permalink)
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Default Re: What part to use?

Thanks a lot, the slideshow was pretty helpful. still wondering, though, can i use other parts of the plant?
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Old 01-18-2013, 07:18 PM   #4 (permalink)
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Default Re: What part to use?

Quote:
Originally Posted by banana13 View Post
Thanks a lot, the slideshow was pretty helpful. still wondering, though, can i use other parts of the plant?
I don't know how many threads came up when you searched this, but I know there are several. I do know you can only use the merristem.. ie the growth point. :^)
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Old 01-18-2013, 08:23 PM   #5 (permalink)
 
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Default Re: What part to use?

TC Male Flower Bananas
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Old 01-18-2013, 08:31 PM   #6 (permalink)
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Default Re: What part to use?

OK, then, so what do people mean when they say that they use the male flower? Do they contain some sort of meristem? Or perhaps it is harder to propagate from them or the other locations mentioned in various places?
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Old 01-19-2013, 02:40 AM   #7 (permalink)
 
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Default Re: What part to use?

It contains undifferentiated cells, which can be induced to form callus and ultimately shoots.

This technique is more advanced and requires different growth regulators, not really for the beginner.

And, yes there are many threads on this topic and others, all the information you could possibly need.
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Old 01-19-2013, 09:39 AM   #8 (permalink)
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Default Re: What part to use?

Thanks sunfish for that thread, and thanks to everyone else too. I guess i will be sticking to corms for now
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Old 01-19-2013, 10:00 AM   #9 (permalink)
 
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Default Re: What part to use?

Some questions: Psuedostem, Meristem, where?
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Old 01-19-2013, 11:27 AM   #10 (permalink)
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Default Re: What part to use?

Quote:
Originally Posted by sunfish View Post
That's the most recent thread I was thinking about. :^)
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Old 01-19-2013, 12:54 PM   #11 (permalink)
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Default Re: What part to use?

thanks again
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Old 01-20-2013, 01:58 PM   #12 (permalink)
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Default Re: What part to use?

Also, what about "nicking corms," as is stated in the description of this part of the forum? I searched it up but cant find anything. does anyone know what that is supposed to mean?
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Old 01-20-2013, 02:46 PM   #13 (permalink)
 
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Default Re: What part to use?

Quote:
Originally Posted by banana13 View Post
Also, what about "nicking corms," as is stated in the description of this part of the forum? I searched it up but cant find anything. does anyone know what that is supposed to mean?
maybe

Corm propagation a success
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