What part to use?

banana13 · 01-18-2013, 05:11 PM

Hello everyone. while i'm not even close to be able to tissue culture my banana yet, I've got a few questions i might as well get cleared up for when that time comes

. Most of them i could probably find the answers for somewhere else, but my biggest question is WHAT PART TO USE?!? I have seen stuff about using meristem from the corm, but some sites have talked about other areas, like the flower. The info i was looking at recently said:

Quote:

Shoot cultures of banana start conventionally from any plant part that contains a shoot meristem, i.e. the parental pseudostem, small suckers, peepers and lateral buds . The apex of the inflorescence and axillary flower buds are also suitable explants for tissue culture initiation.

What is that supposed to mean?
Also, on a similar tangent, I have seem some stuff about nicking and dividing corms. What is that supposed to mean and how do I do that?
Can anyone help me out here?

NeilG · 01-18-2013, 05:43 PM

Just a quick Google and I found this informative slideshow that details the tissue culture process. Hope it helps out!

Tissue culture techniques of Banana

banana13 · 01-18-2013, 07:00 PM

Thanks a lot, the slideshow was pretty helpful. still wondering, though, can i use other parts of the plant?

Abnshrek · 01-18-2013, 07:18 PM

Quote:

Originally Posted by banana13

Thanks a lot, the slideshow was pretty helpful. still wondering, though, can i use other parts of the plant?

I don't know how many threads came up when you searched this, but I know there are several. I do know you can only use the merristem.. ie the growth point. :^)

sunfish · 01-18-2013, 08:23 PM

TC Male Flower Bananas

banana13 · 01-18-2013, 08:31 PM

OK, then, so what do people mean when they say that they use the male flower? Do they contain some sort of meristem? Or perhaps it is harder to propagate from them or the other locations mentioned in various places?

jmoore · 01-19-2013, 02:40 AM

It contains undifferentiated cells, which can be induced to form callus and ultimately shoots.

This technique is more advanced and requires different growth regulators, not really for the beginner.

And, yes there are many threads on this topic and others, all the information you could possibly need.

banana13 · 01-19-2013, 09:39 AM

Thanks sunfish for that thread, and thanks to everyone else too. I guess i will be sticking to corms for now

sunfish · 01-19-2013, 10:00 AM

Some questions: Psuedostem, Meristem, where?

Abnshrek · 01-19-2013, 11:27 AM

Quote:

Originally Posted by sunfish

Some questions: Psuedostem, Meristem, where?

That's the most recent thread I was thinking about. :^)

banana13 · 01-19-2013, 12:54 PM

thanks again

banana13 · 01-20-2013, 01:58 PM

Also, what about "nicking corms," as is stated in the description of this part of the forum? I searched it up but cant find anything. does anyone know what that is supposed to mean?

sunfish · 01-20-2013, 02:46 PM

Quote:

Originally Posted by banana13

Also, what about "nicking corms," as is stated in the description of this part of the forum? I searched it up but cant find anything. does anyone know what that is supposed to mean?

maybe

Corm propagation a success

01-18-2013, 05:43 PM	#2 (permalink)
NeilG Join Date: Jan 2013 Posts: 4 BananaBucks : 381 Feedback: 0 / 0% Said "Thanks" 0 Times Was Thanked 1 Time in 1 Post Said "Welcome to Bananas" 0 Times	Re: What part to use? Just a quick Google and I found this informative slideshow that details the tissue culture process. Hope it helps out! Tissue culture techniques of Banana

01-18-2013, 07:00 PM	#3 (permalink)
banana13 __ Find me on the map! Zone: 7 Join Date: Jan 2013 Posts: 114 BananaBucks : 59,100 Feedback: 0 / 0% Said "Thanks" 116 Times Was Thanked 46 Times in 32 Posts Said "Welcome to Bananas" 7 Times	Re: What part to use? Thanks a lot, the slideshow was pretty helpful. still wondering, though, can i use other parts of the plant?

01-18-2013, 08:23 PM	#5 (permalink)
sunfish Banned sunfish's Photo Gallery Location: San Diego Find me on the map! Zone: 9-11 Name: Tony Join Date: Apr 2008 Posts: 18,429 BananaBucks : 1,641,261 Feedback: 8 / 100% Said "Thanks" 3,210 Times Was Thanked 20,594 Times in 7,760 Posts Said "Welcome to Bananas" 2,716 Times	Re: What part to use? TC Male Flower Bananas

01-18-2013, 08:31 PM	#6 (permalink)
banana13 __ Find me on the map! Zone: 7 Join Date: Jan 2013 Posts: 114 BananaBucks : 59,100 Feedback: 0 / 0% Said "Thanks" 116 Times Was Thanked 46 Times in 32 Posts Said "Welcome to Bananas" 7 Times	Re: What part to use? OK, then, so what do people mean when they say that they use the male flower? Do they contain some sort of meristem? Or perhaps it is harder to propagate from them or the other locations mentioned in various places?

01-19-2013, 02:40 AM	#7 (permalink)
jmoore jmoore's Photo Gallery Location: Lowestoft, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 766 BananaBucks : 159,785 Feedback: 4 / 100% Said "Thanks" 192 Times Was Thanked 799 Times in 373 Posts Said "Welcome to Bananas" 71 Times	Re: What part to use? It contains undifferentiated cells, which can be induced to form callus and ultimately shoots. This technique is more advanced and requires different growth regulators, not really for the beginner. And, yes there are many threads on this topic and others, all the information you could possibly need.

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01-19-2013, 09:39 AM	#8 (permalink)
banana13 __ Find me on the map! Zone: 7 Join Date: Jan 2013 Posts: 114 BananaBucks : 59,100 Feedback: 0 / 0% Said "Thanks" 116 Times Was Thanked 46 Times in 32 Posts Said "Welcome to Bananas" 7 Times	Re: What part to use? Thanks sunfish for that thread, and thanks to everyone else too. I guess i will be sticking to corms for now

01-19-2013, 10:00 AM	#9 (permalink)
sunfish Banned sunfish's Photo Gallery Location: San Diego Find me on the map! Zone: 9-11 Name: Tony Join Date: Apr 2008 Posts: 18,429 BananaBucks : 1,641,261 Feedback: 8 / 100% Said "Thanks" 3,210 Times Was Thanked 20,594 Times in 7,760 Posts Said "Welcome to Bananas" 2,716 Times	Re: What part to use? Some questions: Psuedostem, Meristem, where?

01-19-2013, 12:54 PM	#11 (permalink)
banana13 __ Find me on the map! Zone: 7 Join Date: Jan 2013 Posts: 114 BananaBucks : 59,100 Feedback: 0 / 0% Said "Thanks" 116 Times Was Thanked 46 Times in 32 Posts Said "Welcome to Bananas" 7 Times	Re: What part to use? thanks again

01-20-2013, 01:58 PM	#12 (permalink)
banana13 __ Find me on the map! Zone: 7 Join Date: Jan 2013 Posts: 114 BananaBucks : 59,100 Feedback: 0 / 0% Said "Thanks" 116 Times Was Thanked 46 Times in 32 Posts Said "Welcome to Bananas" 7 Times	Re: What part to use? Also, what about "nicking corms," as is stated in the description of this part of the forum? I searched it up but cant find anything. does anyone know what that is supposed to mean?

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