Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
The Facebook Platform
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 08-10-2012, 12:13 PM   #1 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Some questions: Psuedostem, Meristem, where?

I guess I am a little confused, I am under the impression that the place to take tissue for TC is the pseudostem, or down where the stem meets the corm, correct?

but what is this meristem I am also hearing about?

can someone explain to me or show me a diagram of a banana plant these places:
Pseudostem
meristem
corm(bulbous base of plant?)

I'm getting ready to harvest some pups and wanted to make sure I am going to be using the right areas but have gotten a little confused.

Is the meristem down towards the bottom of the plant where the stem and leaves actually start? or is it at the top?

and the speudostem is what?

I guess you can plant a corm and it will produce pups, but you can't TC just the corm because it won't produce because there is no pseudostem/meristem?

thanks for the help.
Larry
__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 08-10-2012, 12:49 PM   #2 (permalink)
Moderator

 
Gabe15's Avatar
 
Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,643
BananaBucks : 343,013
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was Thanked 7,162 Times in 1,927 Posts
Said "Welcome to Bananas" 8 Times
Default Re: Some questions: Psuedostem, Meristem, where?

In this diagram "gp" (growing point) means meristem.

__________________
Growing bananas in Colorado, Hawaii and Washington since 2004.
Gabe15 is online now   Reply With Quote Send A Private Message To Gabe15
Said thanks:
Old 08-10-2012, 03:42 PM   #3 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Quote:
Originally Posted by Gabe15 View Post
In this diagram "gp" (growing point) means meristem.


So is that the only point I can use to do TC? I must make sure I have that in order to get a viable TC going correct?

And when we talked about quartering a Corm, and planting the quarters, those will grow new pups even though there's no pseudostem? of is the GP part of each of those quarters?

thanks
__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619
Old 08-11-2012, 01:45 AM   #4 (permalink)
 
jmoore's Avatar
 
Location: Lowestoft, UK
Zone: 8a
Name: James
Join Date: Aug 2008
Posts: 746
BananaBucks : 76,573
Feedback: 3 / 100%
Said "Thanks" 190 Times
Was Thanked 762 Times in 355 Posts
Said "Welcome to Bananas" 68 Times
Default Re: Some questions: Psuedostem, Meristem, where?

At the base of each pseudostem you will find a meristem, if you have 2 pseudostems you will have 2 meristems. The meristem is a ball of unspecialised cells, they will either be triggered to become a leaf or a flower bud.

Also at the base of each sheath there is a dormant meristem, this is what you are stimulating to grow when you carry out TC on a plant. They are kept dormant by the dominance of the growing meristem. If the dominant meristem is damaged (or the dominance remove) then another meristem is stimulated to start growing. All governed by growth regulators produced by the plant and that are produced artificially for the purposes of TC.

If you quarter a corm it will only produce a pup where it has an eye, much like potatoes have eyes, so do corms. They look like swellings on the corm that come to a little point. These do not have meristems yet as they haven't produced a pseudostem. These will be no good for TC. However if you cut one off with a bit of corm it will grow into a plant.
jmoore is offline   Reply With Quote Send A Private Message To jmoore
Said thanks:
Old 08-11-2012, 11:27 PM   #5 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Re: Some questions: Psuedostem, Meristem, where?

When I cut into my tissue donors today, I could totally see the different regions indicated in the picture. Thanks!
__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619
Sponsors

Old 08-15-2012, 01:49 PM   #6 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Some questions: Psuedostem, Meristem, Slicing?

I photoshoped some images of one of my cultures to ask if I am using the correct parts or if I need to trim it back further.

This is a TC I did with the merristem cut in half, I outlined the merristem tip with photoshop:



Here's is the diagram I created of said TC, as you can see there are leaf sheaths left on there some cortex and then the merristem in the middle. Is this ok to do leaving sheath on there? or should I cut it down so there's no sheaths left?



Here are some diagrams of possible slices to use, should these be used in this style of cutting? Or should I be using ONLY the merristem? I am also assuming the merristem is not just the tip it also goes deeper than just the very tip, yes?

Slicing the merristem with some of the cortex on the outer cuts, is that ok?


or is it ok to keep some of the other parts of the plant such as the sheath and such?

Also can the cuts be made by slicing vertically in a pattern or does it have to be in a quartered type pattern?

thanks
Larry

__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619
Said thanks:
Old 08-17-2012, 10:41 PM   #7 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Could I get a bump?
Gabe? Jmoore? Bueller?Bueller?
__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619
Old 11-20-2012, 05:37 AM   #8 (permalink)
Relaxin Under the Nanners
 
Hammocked Banana's Avatar
 
Location: Toronto, ON and Peterborough, ON
Zone: 6a and 5a respectively
Name: Brady
Join Date: Jan 2012
Posts: 958
BananaBucks : 37,507
Feedback: 2 / 100%
Said "Thanks" 3,177 Times
Was Thanked 1,153 Times in 553 Posts
Said "Welcome to Bananas" 30 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Bump x2
Hammocked Banana is offline   Reply With Quote Send A Private Message To Hammocked Banana
Said thanks:
Old 11-20-2012, 06:22 AM   #9 (permalink)
Banned
 
Location: San Diego
Zone: 9-11
Name: Tony
Join Date: Apr 2008
Posts: 18,431
BananaBucks : 47,361
Feedback: 8 / 100%
Said "Thanks" 3,210 Times
Was Thanked 20,345 Times in 7,724 Posts
Said "Welcome to Bananas" 2,716 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Bump 3
sunfish is offline   Reply With Quote Send A Private Message To sunfish
Said thanks:
Old 11-20-2012, 12:57 PM   #10 (permalink)
Moderator

 
Gabe15's Avatar
 
Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,643
BananaBucks : 343,013
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was Thanked 7,162 Times in 1,927 Posts
Said "Welcome to Bananas" 8 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Sorry I missed this earlier.

The dome shape that you see there and have labeled as "meristem" is not quite accurate, it is more like what you have labeled as "cortex" along with "growing point". The meristem is a region that spreads laterally between the corm cortex and the pseudostem. What you have labeled as "meristem" is actually more the corm cortex.

I would not recommend making the slices as you have outlined above. The explant should be older and more developed before you subculture it, but when you do, just halve or quarter the whole piece by making a cut directly through from the top of the pseduostem sheaths, down through the meristem, and through the corm. You cannot isolate the meristem tissue really, all you need is to cut pieces that contain some meristem tissue and they will continue to grow. If you were to cut as you out lined above, and threw away the rest of the tissue, you would risk throwing away the meristem.

I recommend taking a piece like you have there and just splitting it into two pieces, if you have it on the right medium, the bud should regenerate into a circular-type mini bud and produce a few more buds too, each of which can be quartered in a similar way as shown below.

__________________
Growing bananas in Colorado, Hawaii and Washington since 2004.
Gabe15 is online now   Reply With Quote Send A Private Message To Gabe15
Old 11-20-2012, 01:58 PM   #11 (permalink)
"Custom User Title"
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 598
BananaBucks : 38,128
Feedback: 10 / 100%
Said "Thanks" 134 Times
Was Thanked 538 Times in 217 Posts
Said "Welcome to Bananas" 1 Times
Default Re: Some questions: Psuedostem, Meristem, where?

Thamks for the bump Tony and Thanks for the info Gabe!! It makes sense now.
__________________
Gros Michel Bananas for Sale
http://www.grosmichelbanana.net

Passion Flower Vines for Sale
http://www.passionflowervine.com

Pineapple Guava Trees for Sale
https://www.pineappleguava.net/
sddarkman619 is online now   Reply With Quote Send A Private Message To sddarkman619
Old 11-28-2012, 08:09 PM   #12 (permalink)
 
edzone9's Avatar
 
Location: Florida Zone 9
Zone: 9
Name: Ed
Join Date: Nov 2012
Posts: 912
BananaBucks : 164,470
Feedback: 1 / 100%
Said "Thanks" 1,135 Times
Was Thanked 895 Times in 406 Posts
Said "Welcome to Bananas" 10 Times
Default Re: Some questions: Psuedostem, Meristem, where?

I Must Admit , All This TC Talk Is Very Ineresting !
Sounds Like A Cool Hobby ...

Good Luck With Your TC !
edzone9 is offline   Reply With Quote Send A Private Message To edzone9
Reply   Email this Page Email this Page






Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
meristem / pseudostem iow sean Main Banana Discussion 4 01-24-2010 03:30 PM
Psuedostem rotten Dean W. Main Banana Discussion 35 09-15-2008 08:22 PM
Rotting psuedostem question harveyc Main Banana Discussion 3 10-28-2007 12:57 AM
I have some questions can you help mike Banana Identification 0 09-27-2007 07:25 PM
Just a few questions Carolina Main Banana Discussion 6 04-12-2006 03:29 PM


All times are GMT -5. The time now is 01:18 AM.





Follow us:
Twitter YouTube

All content © Bananas.org & the respective author.