Hardware.

druss · 05-23-2017, 08:19 AM

I have been looking into the kitchen kulture kits, and I wondered what hardware would be recommended to go with it? Im looking at a flow hood with hepa filter and I am not sure if I should look into a climate control growth cabinet? Is it necessary for plantlet grow out?

Gabe15 · 06-05-2017, 12:44 PM

A proper flow hood is nice, but expensive and not really necessary for hobby TC. Some folks get away with just doing it in an aquarium on its side, wiped down with alcohol immediately before using and careful hand movements while in progress. I made a simple flow hood out of a plastic bin, a box fan, a trash bag, duct tape and doubled-up high efficiency air duct filters, it worked pretty good.

Even if you decide to get a flow hood, you definitely do not need a growth chamber. Keeping the vessels on a shelf or table, out of sunlight under a simple fluorescent light tube in a normal household environment (not too hot, not too cold) is completely acceptable and more or less what professional labs do anyways.

Here is my little set-up I made one summer years ago on break home from university. MY vessels were under a T5 fluorescent grow light fixture which was totally not necessary but I just happened to have it on hand and used it. I was working at the banana tissue culture lab at the university at the time so I borrowed some of the magenta box vessels, but I also used some other random ones I got as free samples when I ordered my media from Phytotech Labs, as well as some standard mason jars.

druss · 06-05-2017, 06:20 PM

Nice job!

sddarkman619 · 09-05-2018, 09:01 PM

This was my lab/flow hood. Made out of a plastic bin with an air filter with a "HEPA" filter put into it. I doubt it was a true HEPA filter but it worked well. I used to have a full on HEPA hood but gave that away years ago.

Duct tape to seal the edges and 90% rubbing alcohol, did the job just fine.

Agar on the left cooling down and getting ready to pour many petri dishes for other types of tissue culturing.

05-23-2017, 08:19 AM	#1 (permalink)
druss amatuer zone bender Location: perth western australia Join Date: Jan 2015 Posts: 323 BananaBucks : 27,635 Feedback: 0 / 0% Said "Thanks" 26 Times Was Thanked 295 Times in 138 Posts Said "Welcome to Bananas" 1 Times	Hardware. I have been looking into the kitchen kulture kits, and I wondered what hardware would be recommended to go with it? Im looking at a flow hood with hepa filter and I am not sure if I should look into a climate control growth cabinet? Is it necessary for plantlet grow out?

06-05-2017, 12:44 PM	#2 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,634 BananaBucks : 341,311 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 7,124 Times in 1,916 Posts Said "Welcome to Bananas" 8 Times	Re: Hardware. A proper flow hood is nice, but expensive and not really necessary for hobby TC. Some folks get away with just doing it in an aquarium on its side, wiped down with alcohol immediately before using and careful hand movements while in progress. I made a simple flow hood out of a plastic bin, a box fan, a trash bag, duct tape and doubled-up high efficiency air duct filters, it worked pretty good. Even if you decide to get a flow hood, you definitely do not need a growth chamber. Keeping the vessels on a shelf or table, out of sunlight under a simple fluorescent light tube in a normal household environment (not too hot, not too cold) is completely acceptable and more or less what professional labs do anyways. Here is my little set-up I made one summer years ago on break home from university. MY vessels were under a T5 fluorescent grow light fixture which was totally not necessary but I just happened to have it on hand and used it. I was working at the banana tissue culture lab at the university at the time so I borrowed some of the magenta box vessels, but I also used some other random ones I got as free samples when I ordered my media from Phytotech Labs, as well as some standard mason jars. __________________ Growing bananas in Colorado, Hawaii and Washington since 2004. Last edited by Gabe15 : 06-05-2017 at 12:47 PM.

06-05-2017, 06:20 PM	#3 (permalink)
druss amatuer zone bender Location: perth western australia Join Date: Jan 2015 Posts: 323 BananaBucks : 27,635 Feedback: 0 / 0% Said "Thanks" 26 Times Was Thanked 295 Times in 138 Posts Said "Welcome to Bananas" 1 Times	Re: Hardware. Nice job!

09-05-2018, 09:01 PM	#4 (permalink)
sddarkman619 "Custom User Title" Find me on the map! Name: Larry Join Date: Jul 2012 Posts: 594 BananaBucks : 35,110 Feedback: 10 / 100% Said "Thanks" 133 Times Was Thanked 535 Times in 215 Posts Said "Welcome to Bananas" 1 Times	Re: Hardware. This was my lab/flow hood. Made out of a plastic bin with an air filter with a "HEPA" filter put into it. I doubt it was a true HEPA filter but it worked well. I used to have a full on HEPA hood but gave that away years ago. Duct tape to seal the edges and 90% rubbing alcohol, did the job just fine. Agar on the left cooling down and getting ready to pour many petri dishes for other types of tissue culturing. __________________ Gros Michel Bananas for Sale http://www.grosmichelbanana.net Passion Flower Vines for Sale http://www.passionflowervine.com Pineapple Guava Trees for Sale https://www.pineappleguava.net/

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