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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 07-02-2019, 03:30 AM   #21 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

its really great to see that their is an platform were farmers can intact with others to discuss their issues and get smart solutions.
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Old 08-27-2019, 05:49 PM   #22 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Excellent writeup and pictures Sddarkman619.

What is your conclusion now after all your testing? Did you have better results using Coco noir than the sawdust?

I want to try this method as well. This last week I became incredibly fascinated with this method. After refining this method, I heard that you can get ~64 plants from one corm.

Thanks!

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Old 08-27-2019, 07:27 PM   #23 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Sddarkman619,

When you pared down the corm, did you cut an 'x' on each bud that you saw? I heard that helps to get more sprouts from that location.

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Old 08-27-2019, 09:50 PM   #24 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

I did have a nice outcome with the coco coir, much better than saw dust or rice hulls. For me at least.
Yes you have to basically kill/damage the bud down to it's merristem in order to get it to put pups out rather than 1 pseudostem.
I have now started using a drill bit instead. seeing how that works.
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Old 08-30-2019, 07:07 AM   #25 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by sddarkman619 View Post

Yes you have to basically kill/damage the bud down to it's merristem in order to get it to put pups out rather than 1 pseudostem.
Most propagation manuals will say to kill or damage the Growing Point in order to remove apical dominance. That's mainly because plants are plentiful and inexpensive and the technique is quick and easy, but obviously the Growing Point can be removed without killing it and that will also remove apical dominance from the remaining rhizome.

Here's a variegated banana that is being used for macropropagation. After the Growing Point was removed it was replanted to start process over again. This probably can go on for eternity using the same growing point.



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Old 08-30-2019, 08:19 AM   #26 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Hi PR-Giants,

Thanks for your reply as well. I read that on top of removing/killing the main growth point, for maximum output, to identify growth points on the sides of the pared corn and slicing an ‘x’ on each one to obtain multiple growths out of each individual growth point. Not too deep as you don’t want to kill that growth point. However, I have not seen any close up pics to identify those growth points. It was referred to it as the PIF technique.

Then as the pups start shooting off, the bigger ones you are supposedly able to cut off (killing) and then you x in that growth point to get more pups from there. Like you mentioned, this process could go on for eternity.

Do you have a picture of your prepared corn, all pared down and ready to go? I am going to try with and expendable Basjoo I have in order to test the methods discussed.

Mcfly
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Old 08-30-2019, 10:11 AM   #27 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Watch this video:

Macropropagation from ProMusa Video Bank on Vimeo.

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Old 08-30-2019, 10:27 AM   #28 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

excellent video. makes you want to try it.
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Old 08-30-2019, 12:39 PM   #29 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

There are many different techniques and understanding the basics will help in choosing what works best in each situation.

One plant can produce thousands of new plantlets but for us maximum output is less desirable than speed.

The variegated rhizome in the top photo will basically activate each those buds in a specific order and allow us to remove a large pup about every 6 days. This is the advantage of having it focus it's resources on just a few buds at a time.

The bottom photo is what happens by mutilating one of those buds.

My first post was to point out that the main plant can be removed and replanted instead of killing it.

I prefer to use clean coarse sand as the medium because it'll last forever, it drains well, has great aeration, and it's easy to keep the humidity perfect.

There are many options with macropropagation and understanding how apical dominance changes is interesting and useful.






Quote:
Originally Posted by mcfly View Post
Hi PR-Giants,

Thanks for your reply as well. I read that on top of removing/killing the main growth point, for maximum output, to identify growth points on the sides of the pared corn and slicing an ‘x’ on each one to obtain multiple growths out of each individual growth point. Not too deep as you don’t want to kill that growth point. However, I have not seen any close up pics to identify those growth points. It was referred to it as the PIF technique.

Then as the pups start shooting off, the bigger ones you are supposedly able to cut off (killing) and then you x in that growth point to get more pups from there. Like you mentioned, this process could go on for eternity.

Do you have a picture of your prepared corn, all pared down and ready to go? I am going to try with and expendable Basjoo I have in order to test the methods discussed.

Mcfly
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Old 08-30-2019, 03:32 PM   #30 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by sddarkman619 View Post
Watch this video:

Macropropagation from ProMusa Video Bank on Vimeo.

Yes, this is one of the videos I saw. Great video. Great solution for that part of the world too. It really solved a problem.
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Recently started:
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Maybe Namwa
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Red Zebrina
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Old 08-30-2019, 03:48 PM   #31 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Great info PR-Giants. Thanks for the pics too. I just need to get this started. Perhaps sometime during this three day weekend I'll have some time.

I don't have much in the way of a controlled environment ie no greenhouse but I have some areas that get less sun. A rubbermaid bin (or other) with some holes in the bottom, bedding cloth, then I'll try once with Coco coir and one with course sand. Place a plastic sheet on top and give it a run.


Quote:
Originally Posted by PR-Giants View Post
There are many different techniques and understanding the basics will help in choosing what works best in each situation.

One plant can produce thousands of new plantlets but for us maximum output is less desirable than speed.

The variegated rhizome in the top photo will basically activate each those buds in a specific order and allow us to remove a large pup about every 6 days. This is the advantage of having it focus it's resources on just a few buds at a time.

The bottom photo is what happens by mutilating one of those buds.

My first post was to point out that the main plant can be removed and replanted instead of killing it.

I prefer to use clean coarse sand as the medium because it'll last forever, it drains well, has great aeration, and it's easy to keep the humidity perfect.

There are many options with macropropagation and understanding how apical dominance changes is interesting and useful.




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Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'

Last edited by mcfly : 08-30-2019 at 04:57 PM.
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