Kreos
08-11-2016, 12:59 PM
This is my little TC experiment in home conditions .
I have no laminar or lab.
media preparation
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg.html)
Explant surface sterilization
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg.html)
My clean "sterile" work area
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg.html)
Have no laminar flow hood so I had 50 % contamination.
Musa basjoo
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg.html)
The final phase
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg.html)
I thought no single banana survive, because the upper part dried up , but after a few days new roots and leafs appeared.
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg.html)
I also menage to TC some other plants mostly Colocasia sp.
I have no laminar or lab.
media preparation
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg.html)
Explant surface sterilization
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg.html)
My clean "sterile" work area
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg.html)
Have no laminar flow hood so I had 50 % contamination.
Musa basjoo
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg.html)
The final phase
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg.html)
I thought no single banana survive, because the upper part dried up , but after a few days new roots and leafs appeared.
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg.html)
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg.html)
I also menage to TC some other plants mostly Colocasia sp.