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Kreos
08-11-2016, 12:59 PM
This is my little TC experiment in home conditions .
I have no laminar or lab.

media preparation
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_155314_zpsoow16wed.jpg.html)

Explant surface sterilization
http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC007831_zpsm0dfkd0x.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151214_180902_zpsfn1lyix6.jpg.html)

My clean "sterile" work area

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00836_zpsrd0itij8.jpg.html)

Have no laminar flow hood so I had 50 % contamination.


Musa basjoo


http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/DSC00908_zpsmoordmzk.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160107_171358_zpschjhydyz.jpg.html)


http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160128_142848_zpsydrxad8b.jpg.html)


http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160302_140510_zpsheyn5ekf.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_160629_zpsugsmvgjz.jpg.html)


The final phase

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160319_175513_zpsqfnodurw.jpg.html)


I thought no single banana survive, because the upper part dried up , but after a few days new roots and leafs appeared.

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104023_zpsnpzoonbu.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160403_104012_zpsrifhstki.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20160508_104633_zpstzvheciv.jpg.html)

I also menage to TC some other plants mostly Colocasia sp.

JP
08-11-2016, 02:24 PM
What did you use?

merce3
08-11-2016, 02:25 PM
Very cool. Where did you get your supplies?

jmoore
08-11-2016, 03:06 PM
Excellent! Well done. That's spurred me on to have another go at TC.

Kreos
08-11-2016, 11:04 PM
Standard MS medium with PGR

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151209_153049_zpscnghohgh.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_153049_zpscnghohgh.jpg.html)

http://i583.photobucket.com/albums/ss279/kreos123/Home%20tissue%20culture/20151209_152147_zpsfyhskm6n.jpg (http://s583.photobucket.com/user/kreos123/media/Home%20tissue%20culture/20151209_152147_zpsfyhskm6n.jpg.html)

a.hulva@coxinet.net
08-12-2016, 10:33 AM
One of the best presentation's I have seen.
Thank you!

Island Brah
08-18-2016, 09:11 AM
That's awesome! You should put together a guide for novices.

Kreos
08-18-2016, 09:22 AM
That's awesome! You should put together a guide for novices.
You can find many TC protocols on net.

http://www.academicjournals.org/journal/AJB/article-full-text-pdf/106134B45706

Island Brah
08-18-2016, 09:24 AM
You can find many TC protocols on net.

http://www.academicjournals.org/journal/AJB/article-full-text-pdf/106134B45706


Looking at all the info in that link makes my brain hurt lol

benbaron
08-25-2016, 03:23 AM
What was the time scale from chop to growing plug?

Kreos
08-25-2016, 04:32 AM
What was the time scale from chop to growing plug?

4 months

Paulzie32
08-31-2016, 04:18 PM
step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?

Kreos
09-01-2016, 07:49 AM
step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?

You can find many TC protocol on internet.
All of my plant dried because not enough humidity.