Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
The Facebook Platform
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 08-11-2016, 12:59 PM   #1 (permalink)
 
Kreos's Avatar
 
Location: Croatia
Zone: 7a
Join Date: Feb 2013
Posts: 41
BananaBucks : 14,210
Feedback: 0 / 0%
Said "Thanks" 67 Times
Was Thanked 87 Times in 23 Posts
Said "Welcome to Bananas" 6 Times
Default My tissue culture experiment

This is my little TC experiment in home conditions .
I have no laminar or lab.

media preparation


Explant surface sterilization




My clean "sterile" work area



Have no laminar flow hood so I had 50 % contamination.


Musa basjoo















The final phase




I thought no single banana survive, because the upper part dried up , but after a few days new roots and leafs appeared.







I also menage to TC some other plants mostly Colocasia sp.

Last edited by Kreos : 08-11-2016 at 01:10 PM.
Kreos is offline   Reply With Quote Send A Private Message To Kreos

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 08-11-2016, 02:24 PM   #2 (permalink)
JP
 
Location: Trois-Rivières, Québec
Zone: 4
Name: JP
Join Date: Nov 2014
Posts: 1,210
BananaBucks : 49,934
Feedback: 3 / 100%
Said "Thanks" 1,868 Times
Was Thanked 1,640 Times in 798 Posts
Said "Welcome to Bananas" 303 Times
Default Re: My tissue culture experiment

What did you use?
JP is offline   Reply With Quote Send A Private Message To JP
Old 08-11-2016, 02:25 PM   #3 (permalink)
 
merce3's Avatar
 
Zone: 9b
Join Date: Oct 2013
Posts: 528
BananaBucks : 39,348
Feedback: 4 / 100%
Said "Thanks" 1,077 Times
Was Thanked 1,037 Times in 371 Posts
Said "Welcome to Bananas" 256 Times
Default Re: My tissue culture experiment

Very cool. Where did you get your supplies?
__________________
merce3 is offline   Reply With Quote Send A Private Message To merce3
Old 08-11-2016, 03:06 PM   #4 (permalink)
 
jmoore's Avatar
 
Location: Lowestoft, UK
Zone: 8a
Name: James
Join Date: Aug 2008
Posts: 734
BananaBucks : 67,103
Feedback: 3 / 100%
Said "Thanks" 184 Times
Was Thanked 744 Times in 345 Posts
Said "Welcome to Bananas" 68 Times
Default Re: My tissue culture experiment

Excellent! Well done. That's spurred me on to have another go at TC.
jmoore is offline   Reply With Quote Send A Private Message To jmoore
Old 08-11-2016, 11:04 PM   #5 (permalink)
 
Kreos's Avatar
 
Location: Croatia
Zone: 7a
Join Date: Feb 2013
Posts: 41
BananaBucks : 14,210
Feedback: 0 / 0%
Said "Thanks" 67 Times
Was Thanked 87 Times in 23 Posts
Said "Welcome to Bananas" 6 Times
Default Re: My tissue culture experiment

Standard MS medium with PGR



Kreos is offline   Reply With Quote Send A Private Message To Kreos
Sponsors

Old 08-12-2016, 10:33 AM   #6 (permalink)
Al's Manini Farm
 
Location: Edmond, OK
Zone: 73034
Name: Al
Join Date: Oct 2014
Posts: 433
BananaBucks : 25,593
Feedback: 0 / 0%
Said "Thanks" 3,495 Times
Was Thanked 711 Times in 294 Posts
Said "Welcome to Bananas" 383 Times
Default Re: My tissue culture experiment

One of the best presentation's I have seen.
Thank you!
__________________
Al
Al's Manini Farm
a.hulva@coxinet.net is offline   Reply With Quote Send A Private Message To a.hulva@coxinet.net
Said thanks:
Old 08-18-2016, 09:11 AM   #7 (permalink)
 
Island Brah's Avatar
 
Zone: 7b & 8a Border
Name: Island Brah
Join Date: Jun 2016
Posts: 157
BananaBucks : 15,294
Feedback: 0 / 0%
Said "Thanks" 417 Times
Was Thanked 264 Times in 90 Posts
Said "Welcome to Bananas" 2 Times
Default Re: My tissue culture experiment

That's awesome! You should put together a guide for novices.
__________________
Growing: Basjoo, Tall Namwah, Tall Orinoco, Manzano, FHIA-1 Agristarts Goldfinger, & Veinte Cohol
Island Brah is offline   Reply With Quote Send A Private Message To Island Brah
Said thanks:
Old 08-18-2016, 09:22 AM   #8 (permalink)
 
Kreos's Avatar
 
Location: Croatia
Zone: 7a
Join Date: Feb 2013
Posts: 41
BananaBucks : 14,210
Feedback: 0 / 0%
Said "Thanks" 67 Times
Was Thanked 87 Times in 23 Posts
Said "Welcome to Bananas" 6 Times
Default Re: My tissue culture experiment

Quote:
Originally Posted by Island Brah View Post
That's awesome! You should put together a guide for novices.
You can find many TC protocols on net.

http://www.academicjournals.org/jour...f/106134B45706
Kreos is offline   Reply With Quote Send A Private Message To Kreos
Said thanks:
Old 08-18-2016, 09:24 AM   #9 (permalink)
 
Island Brah's Avatar
 
Zone: 7b & 8a Border
Name: Island Brah
Join Date: Jun 2016
Posts: 157
BananaBucks : 15,294
Feedback: 0 / 0%
Said "Thanks" 417 Times
Was Thanked 264 Times in 90 Posts
Said "Welcome to Bananas" 2 Times
Default Re: My tissue culture experiment

Quote:
Originally Posted by Kreos View Post
You can find many TC protocols on net.

http://www.academicjournals.org/jour...f/106134B45706

Looking at all the info in that link makes my brain hurt lol
__________________
Growing: Basjoo, Tall Namwah, Tall Orinoco, Manzano, FHIA-1 Agristarts Goldfinger, & Veinte Cohol
Island Brah is offline   Reply With Quote Send A Private Message To Island Brah
Said thanks:
Old 08-25-2016, 03:23 AM   #10 (permalink)
 
Location: UK (up north)
Join Date: Feb 2014
Posts: 43
BananaBucks : 6,302
Feedback: 0 / 0%
Said "Thanks" 8 Times
Was Thanked 50 Times in 25 Posts
Said "Welcome to Bananas" 1 Times
Default Re: My tissue culture experiment

What was the time scale from chop to growing plug?
benbaron is offline   Reply With Quote Send A Private Message To benbaron
Said thanks:
Old 08-25-2016, 04:32 AM   #11 (permalink)
 
Kreos's Avatar
 
Location: Croatia
Zone: 7a
Join Date: Feb 2013
Posts: 41
BananaBucks : 14,210
Feedback: 0 / 0%
Said "Thanks" 67 Times
Was Thanked 87 Times in 23 Posts
Said "Welcome to Bananas" 6 Times
Default Re: My tissue culture experiment

Quote:
Originally Posted by benbaron View Post
What was the time scale from chop to growing plug?
4 months
Kreos is offline   Reply With Quote Send A Private Message To Kreos
Said thanks:
Old 08-31-2016, 04:18 PM   #12 (permalink)
 
Paulzie32's Avatar
 
Location: Tampa Area - Lutz/Land O'Lakes
Zone: 9b
Name: Paul
Join Date: Jan 2016
Posts: 135
BananaBucks : 14,658
Feedback: 0 / 0%
Said "Thanks" 689 Times
Was Thanked 120 Times in 53 Posts
Said "Welcome to Bananas" 17 Times
Default Re: My tissue culture experiment

step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?
__________________
Banana-na - Doo Dooo Do-do-doo
Banana-na - Do-do-do-do-dooo


Have:
Mysore - Rajapuri - TT - Blue Java - Orinoco - Hua Moa - Gros Michel - Dwarf Red - Dwarf Namwah - (Red?) Iholena - Dwarf Brazilian - Pisang Klotek - Veinte Cohol - Double Mahoi - California Gold - Velutina - a Pineapple flavored type (Pisang Raja perhaps?) - 2 unknown dwarfs, one is probably a SDC and the other probably another Dwarf Namwah... time will tell.
I think I've gone Bananas!
Paulzie32 is offline   Reply With Quote Send A Private Message To Paulzie32
Said thanks:
Old 09-01-2016, 07:49 AM   #13 (permalink)
 
Kreos's Avatar
 
Location: Croatia
Zone: 7a
Join Date: Feb 2013
Posts: 41
BananaBucks : 14,210
Feedback: 0 / 0%
Said "Thanks" 67 Times
Was Thanked 87 Times in 23 Posts
Said "Welcome to Bananas" 6 Times
Default Re: My tissue culture experiment

Quote:
Originally Posted by Paulzie32 View Post
step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?
You can find many TC protocol on internet.
All of my plant dried because not enough humidity.
Kreos is offline   Reply With Quote Send A Private Message To Kreos
Reply   Email this Page Email this Page






Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
What to do with new tissue culture?? Narnia Tissue Culturing & Other Propagation Techniques of Banana Plants 14 05-03-2012 08:35 PM
what exactly is tissue culture? ArchAngeL01 Tissue Culturing & Other Propagation Techniques of Banana Plants 20 09-13-2009 08:32 PM
Tissue culture in the UK marksbananas Tissue Culturing & Other Propagation Techniques of Banana Plants 6 09-09-2009 04:57 PM
Re: Tissue Culture juniorfarmer Tissue Culturing & Other Propagation Techniques of Banana Plants 4 03-16-2009 07:52 PM
Going to buy the Tissue Culture kit mrbungalow Tissue Culturing & Other Propagation Techniques of Banana Plants 34 03-16-2007 10:24 AM


All times are GMT -5. The time now is 09:14 PM.





Follow us:
Twitter YouTube

All content © Bananas.org & the respective author.