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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 08-11-2016, 12:59 PM   #1 (permalink)
 
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Default My tissue culture experiment

This is my little TC experiment in home conditions .
I have no laminar or lab.

media preparation


Explant surface sterilization




My clean "sterile" work area



Have no laminar flow hood so I had 50 % contamination.


Musa basjoo















The final phase




I thought no single banana survive, because the upper part dried up , but after a few days new roots and leafs appeared.







I also menage to TC some other plants mostly Colocasia sp.

Last edited by Kreos : 08-11-2016 at 01:10 PM.
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Old 08-11-2016, 02:24 PM   #2 (permalink)
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Default Re: My tissue culture experiment

What did you use?
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Old 08-11-2016, 02:25 PM   #3 (permalink)
 
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Default Re: My tissue culture experiment

Very cool. Where did you get your supplies?
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Old 08-11-2016, 03:06 PM   #4 (permalink)
 
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Default Re: My tissue culture experiment

Excellent! Well done. That's spurred me on to have another go at TC.
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Old 08-11-2016, 11:04 PM   #5 (permalink)
 
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Default Re: My tissue culture experiment

Standard MS medium with PGR



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Old 08-12-2016, 10:33 AM   #6 (permalink)
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Default Re: My tissue culture experiment

One of the best presentation's I have seen.
Thank you!
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Old 08-18-2016, 09:11 AM   #7 (permalink)
 
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Default Re: My tissue culture experiment

That's awesome! You should put together a guide for novices.
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Old 08-18-2016, 09:22 AM   #8 (permalink)
 
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Default Re: My tissue culture experiment

Quote:
Originally Posted by Island Brah View Post
That's awesome! You should put together a guide for novices.
You can find many TC protocols on net.

http://www.academicjournals.org/jour...f/106134B45706
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Old 08-18-2016, 09:24 AM   #9 (permalink)
 
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Default Re: My tissue culture experiment

Quote:
Originally Posted by Kreos View Post
You can find many TC protocols on net.

http://www.academicjournals.org/jour...f/106134B45706

Looking at all the info in that link makes my brain hurt lol
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Old 08-25-2016, 03:23 AM   #10 (permalink)
 
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Default Re: My tissue culture experiment

What was the time scale from chop to growing plug?
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Old 08-25-2016, 04:32 AM   #11 (permalink)
 
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Default Re: My tissue culture experiment

Quote:
Originally Posted by benbaron View Post
What was the time scale from chop to growing plug?
4 months
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Old 08-31-2016, 04:18 PM   #12 (permalink)
 
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Default Re: My tissue culture experiment

step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?
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Old 09-01-2016, 07:49 AM   #13 (permalink)
 
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Default Re: My tissue culture experiment

Quote:
Originally Posted by Paulzie32 View Post
step by step Details would be great! Looks like the mediums are only about 12 euros. pretty affordable.

Why do you think all your plants dried out? Not humid enough? How is the one plant doing now?
You can find many TC protocol on internet.
All of my plant dried because not enough humidity.
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