Hi Pineapplefarmer,
I'm following Novi's protocol using MS, Murashige & Skoog Basal Medium, that's the nutrients, along with 5 mg/L NAA and 2 mg/L BAP.
NAA is 1-Naphthaleneacetic acid. BAP is 6-Benzylaminopurine. These are the two plant growth regulators you need to add to the media. You can get this already in a solution so adding them to the media isn't very difficult.
Agar, like jmoor said, can be found in health food stores. I purchased 56 grams from Amazon for less than $5. You only need to add 7.5 grams of powered agar per liter of media so it will make a fair amount. It's pretty difficult to go through all of the steps to make the media in a post. These are getting old but I did put some videos on YouTube about my experience getting started with tissue culture. Maybe they would help.
YouTube - fbt2007's Channel
I use canning jars for bananas and other large plants. They work nice because of the extra headroom. If you use them, drill or puch a small 1/8th inch hole in the lid and cover it with a round band-aid. This allows for air exchange. Cover the lid with a wrap of aluminum foil to help keep contamination from settling on the band-aid and getting in the jar.
