damaclese

Scot this sounds like an ideal situation to use a Small aquarium maybe that way the dishes could be set in it and you could pump the hole thing full of CO2 and you wouldn't have to fuss with cutting the dishes and with the hold thing sealed theirs not much risk for contamination how are you going to filter the Co2 id recommend A UV chamber and you will get the added benefit of some small amounts of O3 which is a nice Little anti bacterial gas if its in controlled amounts but i don't know what the optimum would be of if that would retard growth

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Michael_Andrew

Richard

Yes, there is more than one paper that discusses this in the context of the genetics and heritage of true quadriploids and triploids. Basically, if LMN was produced by LM x LN, then some combination of factors from L, M, N will be needed for successful pollination of LMN.

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Chironex

Now why didn't I think of that? I could actually just use a huge zip lock bag! As a matter of fact, I have some. God, I am such a dork sometimes, it amazes me. Thanks Pauly! I am not concerned with contamination as these are seeds, therefore no filtration is needed.
Do you have any tubing? I figure that I will need to buy a stopper and a hard plastic tube a few inches long, plus about 2-3 ft of 1/4 inch tubing. Then, I will set up a CO2 generator using yeast in a pyrex or nalgene bottle. Genius!

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Scot

Chironex

Is anyone else having trouble playing this video? It starts and i can hear her, but shortly after it begins, the screen goes black.

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damaclese

yes i hapin to have some tubing its drip line i was going to ues for the garden but im sure i can spair a few ft of it and since im going to the high presure spray systom its probley not even going to get used got drip emiters to if you need any LOL

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damaclese

I can only imagine what Scots laboratory is going to look like with 50 zip lock balloons and a bunch of tubing and bottles of yest percolating away good thing the police cant see in God only knows what they will think of it LOL

Um Ya Osifer its Banana seeds ya Yest can you believe that LOL

Scot spreed out across the hood of a police car Better keep some extra money around Scoty LOL

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Michael_Andrew

Same experiment different video.

Michael_Andrew

Ok Scot I made that Co2 Generator. Took about 3 minutes with stuff I had laying around. Just drilled a hole in the lid just a bit smaller than some tubing I had. I shaved down the end of the tubing and held it under hot water to get it soft and pushed into the hole. It fit snug so I don't think I need any kind of sealant. Dumped in some Big Chief and some warm water and mixed up. Poured in some regular baking yeast and added some yeast nutrient just cause I had it laying around. In just a few minutes it putting out a bubble every three seconds. I'm gonna let this flow over one of my seedlings and see what happens. According to this site I should double the growth rate.


The Heartland Institute - Environment & Climate News Article

Chironex

Michael, I bet that yours was a whole lot cheaper than the one on eBay. Yikes!
Co2 GENERATOR GROW HYDROPONIC LED HPS CARBON DIOXIDE - eBay (item 330301219057 end time Jan-23-09 09:25:56 PST)

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Scot

jmoore

Don't forget to change the yeast and sugar as the CO2 production drops off because the yeast will become denatured by the alcohol it will produce. Also too high a sugar concentration will kill the yeast. Aim for less than 50%.

griphuz

Ehm, just another idea maybe,...
The biological way to make CO2 is good, but not really constant.
There are also chemical ways to make CO@, just by adding an acid to Soda (Sodium carbonate) or Calcium carbonate.
Preferrably use a non gassious acid (so not hydrochloric acid, but rather citric acid e.g.) to avoid traces in your mixture.

Or you could just use those ampul-thins they put in the 'make-your-own-soda-drink' machines,...
Kind regards,
Remko.

jmoore

Adding acid to a carbonate/bicarbonate is sound, but you would have to keep adding acid and carbonate. The soda stream cannisters are also good, but you would need a regulator to release the gas at a constant flow. I think on balance the yeast is good providing it is working at its optimum.

Oh unless it's a one shot system - fill up the baggies with CO2 and away you go. In which case the acid to a carbonate/bicarbonate would be best.

Chironex

There are some considerations:
1. Too much CO@ will probably be inhibitive to growth
2. The acid vapors should not enter the bag.

I just want a light, gradual, continuous flow for several days.

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griphuz

Another option would be a completely controlled set of CO2 inlet as used in many planted aquariums.
I know my brother has a system like that, 'feeding' the plants the dissolved CO2 as it were. This is just a small gastank with CO2 (available @ the petstore) with a controlled outlet.

Should you work with either the bio-way or the chemical way, it would be wise to bubble the produced CO2 through some cold water, and next through a piece of cotton to prevent other things than CO2 comming through, and maintain a sterile environment.
It would look a little like this;


The CO2 can be bubbled through a liquid medium, or just let free in the air part of the set-up, I don't know what the intention is here?
Kind regards,
Remko.

Chironex

This is more like what I was thinking. So how much yeast and how much sugar? Does anyone have a good feel for this? I am not seeking a CO2 generator that emits huge amounts - more like a trickle of gas, slow and steady. The bags are about 2-3 gallons (16-20 liters) in size. So not much gas is needed, just enough to raise the ambient CO2 levels inside the bags to approximately 15% v/v.

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damaclese

Scot being a chef and doing a grate deal of baking i use yeast all the time typically to a packet which is approx .5oz of dry active yeast you add 1tsp of sugar and this yeast typically will feed for up to 24hr of that amount of sugar although if I'm creating a sour dour which takes up to 7 days i add !tsp of sugar ever 2 days to keep the yeast active a vary small amount of yeast can make a vary large amount of gas this probably doesn't relate all that well to what you are trying to do but its a start and as some one pointed out all ready to much sugar will kill the yeast and after a few days the alcohol will start to build up but all you have to do is strain it throw a coffee filter and scrape whats left back in to the jar and add more luke warm water and some sugar and you could be ready to go again one thing i know about yeast is its every were and it grows at the drop of a hat you will have to keep it warm about 86 deg is a pretty good rate you can go higher but do you really need to you just need a smal amout of gas

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Chironex

Perhaps simply exhaling into the bag a few times a day would do the trick. If I hold my breath for several seconds, I can increase the CO2 and simply blow into the bag and seal it quickly.
Any thoughts?

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griphuz

No offence Scot, but we do waht to keep thing sterile-ish,....right?

Chironex

It's not really necessary as these are intact seeds. Actually, they are in more sterile medium than if in regular soil. They are also inside of enclosed petri dishes within the bag, so there really isn't much that can happen. To put your mind at ease, I did not exhale into the bags. I simply opened a fresh can of cola and sealed the open can inside of the plastic bag. (If this were in embryo rescue or tissue culture, then yes, it would be necessary to keep things as sterile as possible.)

I may devise a way to containerize TC jars and keep them in a controlled atmosphere supplemented with CO2 at the proper amounts. But that will have to wait for more funds. I would need to regulate the CO2 to maintain the proper parts per million within the chamber. That sounds like expensive equipment and gauges.

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