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Search: Posts Made By: taner01
Forum: Tissue Culturing & Other Propagation Techniques of Banana Plants 07-26-2018, 06:59 AM
Replies: 5
Views: 2,427
Posted By taner01
Re: Contamination problem

Although I tried most things (PPM, NaClO, EtOH, fungucide), I could not prevent the contamination.

could you help me?
Forum: Tissue Culturing & Other Propagation Techniques of Banana Plants 06-19-2018, 01:11 PM
Replies: 5
Views: 2,427
Posted By taner01
Re: Contamination problem

Thanks for your answer.
Our laminar cabinet is quite new.
Plant materials are G.nain. I use rhizomes as explant.
My surface sterilization work; 15% NaClO (30 min.) and 70% EyOH (5 min.).
The tools...
Forum: Tissue Culturing & Other Propagation Techniques of Banana Plants 06-16-2018, 02:57 AM
Replies: 5
Views: 2,427
Posted By taner01
Re: Contamination problem

I use an autoclave to sterilize the media and tools.
I transfer the plants to the media using laminar flow hood .
Within 1 week most plants die due to contamination.
Forum: Tissue Culturing & Other Propagation Techniques of Banana Plants 06-12-2018, 04:23 AM
Replies: 5
Views: 2,427
Posted By taner01
Contamination problem

I am trying to micropropagate, but there are usually contamination in jars.
I am performing intensive sterilization with NaClO and EyOH.
what else can I do?
https://i.hizliresim.com/5ydVyd.jpg...
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