Quote:
Originally Posted by Chironex
I understand, but it is still like peeling the leaves from an artichoke except on a much smaller scale. You can see one of my photos of the a.m. here:
This one is sitting in a baby food jar Magenta cap. So, I would make horizontal slices off the top .5 mm as I understand it. Would you suggest making subsequent .5 mm cuts from the remaining a.m. to initiate separate cultures? I suppose it couldn't hurt. I just don't know if I can slice it thin enough without using a microtome. |
don't make any cutting or slicing. you may want to peel off more of the leaves and expose the apical dome or meristematic region . poke the apex with scalpel blade to inflict wound and let the whole apex get established in the media. you don't need to separate cultures. if you want more cultures then excise apex from another suckers. the idea is to establish the aeae in in vitro culture and initiate axillary buds in a natural way but with the help of hormone to speed up the growth of the buds.