Quote:
Originally Posted by Chironex
Hi Eden! I am particularly glad that you joined us here. As an amateur tissue culturer I am happy to be able to learn more about new studies and sharing of techniques.
I have been experiencing several challenges in embryo rescue, finding a good medium to promote growth. I would also like to find out a technique for some of the Ensete varieties such as ventricosum Maurelii and perrieri. Apparently there has been little success with traditional M&S medium with Ensete. I may also have access to E. v. 'Montebeliardii' which is far more rare than 'Maurelii'. I have read some research on a newer medium, but wondered about any experience that you may have.
Welcome to Bananas.org!
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Hi Scot,
I have no experience in embryo rescue but i thought it is not so difficult as long as the seed is viable. i did immature embryo rescue on corn so this shouldn't be a problem. i know one media formulation for germination of banana embryos from somatic cells initiated through somatic embryogenesis. try this and tell me if it works: MS salts + Morel and Westmore Vitamins (100 mg/L myo-inositol, 1 mg/L thiamine-hydrochloride, 1 mg/L nicotinic acid, 1 mg/L calcium panthotenate, 0.01 mg/L biotin, and 10 mg/L folic acid) + 0.045 mgL BAP + 0.2 mg/L IAA + 30 g/L sucrose, pH 5.7.
aloha.....
eden