today i did tc bananas from male flower and take photo for you all. enjoy it and wish me luck..
step 1. prepare the bananas flower. in this case i have musa velutina (no 1 and 2), new hybrid (3), ensente glucam (4),
Step 2 throw out the skin/layer of flower (what term?) until its only about3-4 cm length and soak in bleach 15 % for 10 minute and then rinse with sterile distilled water 5 times
step 3. open the each layer and take its male flower. best explant is flower in layer no 8 -16 from tip. flower no 1-7 and more than 16 is not good. its callus not embrionic. so cant to turn be shoot.
step 4 put the male flower in media MA. wait 1 month or more until they getting callus. and we can do subculture in same media to get more callus or put in liquid media if do tc cell suspension, or put in shoot induction media.
well, i will post pic later if there is good progress. hah.. i apologize my english is limited to explaining this thread. i hope you all understand what i mean. hah.. tc male flower more complicated than normal tc using the corm. must be carefull. its explant is so little
regards