step by step Tissue Culture Pineapple
 

I did special Tc today for you org. step bus tep tissue culture pineapple. hope can help you interested with tc pineapple
step 1. prepare the crown of pineapple. sometime crown has few baby.

step 2. throw out the leaf until the last/youngest leaf

step 3. soak in bleach 10 % for 10 minute

step 4. cut vertically become 4 if the stem big enough and become 2 if little or do not cut if too little.

step 5. put in the jars with media N5B2 (MS with 5 mg NAA and 2 mg BAP) to get alot shoot. but if want they growth (elongation) moved in media MS with 2 mg BAP and 1 mg IBA

step 6. after 6-8 weeks explant will growth become alot shoot. you can do subculture with same media N5B2

step 7. in 2 next month shoot will growth. very beautiful. isnt it?


regards

Re: step by step Tissue Culture Pineapple
 

wooooooo very interesting novi!!!! i will have to do this some time!

Re: step by step Tissue Culture Pineapple
 

Very cool! Thanks for posting this. I may have to try this now someday.

Re: step by step Tissue Culture Pineapple
 

Thank you for posting this Novisyatria!! That is good information. :2239:

Re: step by step Tissue Culture Pineapple
 

WONDERFUL NOVI!!!

Thank you!!! :D ~Cheryl

Re: step by step Tissue Culture Pineapple
 

Yes very cool, I would love to try it Thanks Novi

Re: step by step Tissue Culture Pineapple
 

awsome, thanks for sharing

What do you do after step 7... Do you cut the growth off at its base and put it in rooting hormone, then soil, Or do you cut off the growth with a little bit of the base left and put it in rooting hormone...

Thanks for helping me :D

Re: step by step Tissue Culture Pineapple
 

blake, i can do both. move the shoots in to rooting media and than in soil. and also can cut the shoot and put in same media for shoot multification. so i can still have shoots stock.

Re: step by step Tissue Culture Pineapple
 

Great!!
Thanx 4 sharing pak novi :)
knowledge is power!!

Re: step by step Tissue Culture Pineapple
 

Thanks Novi! This is great. I'm going to try this today!

Re: step by step Tissue Culture Pineapple
 

Thank You Novi! I have several varieties that I will be mass propagating very soon. Your pictures have helped me understand the process much better than I did after reading about it. I will also start my tc's using the same media formulations that you prescribe here. Thanks again!

Re: step by step Tissue Culture Pineapple
 

Cool!!! It's just like TC'ing Bananas!

I'm working on my pineapple right now. I'll post some picts in a few minutes.

Thanks again Novi!!! This is awesome!

Re: step by step Tissue Culture Pineapple
 

your welcome and post your progrees soon ok

Re: step by step Tissue Culture Pineapple
 

Okay, I'm done cutting the pineapple up. And to think there is a ham in the freezer :nanadrink:


I had this growing in a pot outside. I cut the top off of a pineapple and stuck it ontop of of some dirt in the pot. The frost got to it. I thought it was a goner. The outer leaves died but the inner leave survived. Here is the sad looking pineapple. At least it gave me 10 explants! :woohoonaner:

Sad Pineapple

Cleaning of the dirt

There's something alive under all of that:goteam:

Hacking away at it.

It tastes like chicken:ha:

Into the disinfecting solution

10 Explants:woohoonaner:


:drum:

Thanks Novi!!!!!

Re: step by step Tissue Culture Pineapple
 

cool pic Frank. good luck. and post after the explantgrowth ok

Re: step by step Tissue Culture Pineapple
 

Hi Novi,

Will do. That was fun! Now to see if they grow :drum:

Like you said, it is a thing of beauty to see plants grow from little pieces.

Now I'm going try caladiums. Your pictures of them look fantastic. :goteam:

Re: step by step Tissue Culture Pineapple
 

This is a great thread!.....keep up the good work guys:bananas_b

Re: step by step Tissue Culture Pineapple
 

Sorry everybody. I´m still out at the countryside: The Great Savannah!
I´ll post some pics next week. Great job Novi! Now I´m in a rush to get home ( that´s where I have my products ) Thanks a lot.

Re: step by step Tissue Culture Pineapple
 

I wanna do tissue culture of pineapples and was wondering were to buy the little jars and from what I could find the media that novi refers to is different kind of hormones.
Please help me in successful tissue culture
thanks Brian

Re: step by step Tissue Culture Pineapple
 

Hi Brian,

Canning jars work well.

As for the media and plant growth regulators, you can get them from Carol at Home Tissue Culture* Group

I think you will find her shipping charges much lower than the TC supply houses. You will find her email address on the site if you have any questions.

Frank

Re: step by step Tissue Culture Pineapple
 

Have you successfully used any of these products for pineapple tissue culture?If not which would you or anyone that has used any of there products
suggest?Other than the hormones would you or anyone recommend anything else?With the jars what's the best method of keeping everything sterile from start to finish?
Thanks for EVERYONE'S help to a newbie,and yes I've done a little research first.

Re: step by step Tissue Culture Pineapple
 

You can buy a lot of the growth regulators from this guy Benzylaminopurine they come as powders so you need a bit of know how as far as chemistry goes and getting them to dissolve is a bit of a pain in the backside, but it's by far the cheapest way of doing things.

The agar is a vegetarian alternative to gelatine and can be purchased from health food shops or on line. As for the containers, yes canning jars or Kilner jars are a good thing.

Re: step by step Tissue Culture Pineapple
 

I have used baby food jars.

Re: step by step Tissue Culture Pineapple
 

do i need anything other than the hormones,I dont understand agar either?

Re: step by step Tissue Culture Pineapple
 

Hi Pineapplefarmer,

I'm following Novi's protocol using MS, Murashige & Skoog Basal Medium, that's the nutrients, along with 5 mg/L NAA and 2 mg/L BAP.

NAA is 1-Naphthaleneacetic acid. BAP is 6-Benzylaminopurine. These are the two plant growth regulators you need to add to the media. You can get this already in a solution so adding them to the media isn't very difficult.

Agar, like jmoor said, can be found in health food stores. I purchased 56 grams from Amazon for less than $5. You only need to add 7.5 grams of powered agar per liter of media so it will make a fair amount. It's pretty difficult to go through all of the steps to make the media in a post. These are getting old but I did put some videos on YouTube about my experience getting started with tissue culture. Maybe they would help.
YouTube - fbt2007's Channel

I use canning jars for bananas and other large plants. They work nice because of the extra headroom. If you use them, drill or puch a small 1/8th inch hole in the lid and cover it with a round band-aid. This allows for air exchange. Cover the lid with a wrap of aluminum foil to help keep contamination from settling on the band-aid and getting in the jar.

Re: step by step Tissue Culture Pineapple
 

Thanks so much

Re: step by step Tissue Culture Pineapple
 

Frank
can you upload new pic of your pineapples Tc? btw i have 8 type now in jars

Re: step by step Tissue Culture Pineapple
 

you have 8 different types of pineapples?
I want one of each type

Re: step by step Tissue Culture Pineapple
 

Does it matter if you the concentrated media or one with or without nutrients?
theres like a whole page of them...
link

MS-Based Salts & Media | PhytoTechnology Laboratories

Re: step by step Tissue Culture Pineapple
 

Hi Novi!!!

I had a hard time finding a nice "green" pineapple. I bough a couple that were too ripe and didn't realize it until I tried to trim them.

I really want to say THANK YOU!!! :bananas_b for your help with this. My pineapple explant has started to put on new leaves. I probably should have sliced it in half or quartered it like you did. Next time. For now I'll just enjoy watching this one develop.

Re: step by step Tissue Culture Pineapple
 

Franck: congrats. please upload new pic
pineapplefarmerwannabe: i want sell to you a pineapple plantlet but hard to send it to overseas

Re: step by step Tissue Culture Pineapple
 

An experienced pineapple grower that lives nearby told me that rather than planting the 'crown' that you cut off the pineapple, get one of the plantlets that develop between the upper leaves just below the fruit. Once those are planted, they develop into a mature plant much more quickly than those grown from the crown.

Re: step by step Tissue Culture Pineapple
 

Hi Novi,

Here's some picts of my baby pineapples. When I divided the original one up I put a couple of young leaves in jars. Those started to develop callus and multiply too. I read it was done before but was happy to see it.

These are still in the multiplying stage. After I get enough I'll put them in the shoot enlongation stage.







Thanks Novi!!!

Re: step by step Tissue Culture Pineapple
 

you did it Frank. congrats.

Re: step by step Tissue Culture Pineapple
 

I would have never figured out how much NAA to use without your help.
Thanks so much for your help. :nanadrink:

Re: step by step Tissue Culture Pineapple
 

Bravooo.. and thanks for your pics... i’ll try to TC pineapple first:0517:

Re: step by step Tissue Culture Pineapple
 

I watched a youtube video of a pineapple grown from seeds. What type of pineapple do you get from a seed. Is it the same as the mother plant?

Re: step by step Tissue Culture Pineapple
 

if from seeds could be different from its mother plant

Re: step by step Tissue Culture Pineapple
 

Trying pineapple tissue culture. The bottom of the explant turns brown, and top green is this normal? The samples are only 2 weeks old.

Re: step by step Tissue Culture Pineapple
 

which one is it is the meristem. im doing tissue culture right now and im confuse which part of the pineapple to be cultured in the media.

Re: step by step Tissue Culture Pineapple
 

LOOKS easy, HOPE so! THANKS:

Re: step by step Tissue Culture Pineapple
 

Aloha, this was a great read. Starting with something simple like pineapple is a great way to learn and encourage others to start their own TC practice.

My wife has experience in Mycology, but we have never had the chance to try other flora. I would like to set up a station but we are not yet at that point.

Seeds. We bought a white pineapple at our market. One segment contained several seeds. These next few days I was going to make time to plant them. Curious question. I understand it may take 5 to 8 years to fruit. Also, assuming the other around it are seedless... Could they be from stress? Or maybe an old variety? Or just a case of the plant trying to live on?

Anyway... everyone should TC. It would make the world a better place. My goal is to some day TC my Fe'i... so I can see those little plants grow.


Mahalo :nanadrink:

Re: step by step Tissue Culture Pineapple
 

Hey Novy
Do you use any preservative in your media. l am having bacteria which l cant get rid off each time l initiate. Have you ever used PPM or give me a tip of how to get rid of bacteria