looks so simple
 

hello guys.
when I read the articles about TC it all seems so simple.
But is it?
I'm from Belgium and I must say my english is not bad (talking), but there are many technical words and abbreviations that I do not understand.
Especially the composition of the medium remains a mystery.
Products from the USA or Canada orders is nearly impossible.
Have found a supplier in the Netherlands but without knowing what I need to compile the medium is also an impossible task.
example.
The composition of the agar.
banana
flowers
vegetables, ...
are these the same?
can you give me the exact composition of the proper names of products for bananas tc please.
I found a Dutch book on the internet which I definitely going to order.
is from 1970.
plants in test tubes

thanks.

Re: looks so simple
 

Hi

The make up for banana TC seems to be different to other plants or flowers.

From what I've read, found out myself you need:

agar (can be bought from health food shops known as agar agar) about 8 grams per litre of water.
Sucrose (normal sugar that you would put in your tea, sorry I'm from England) about 30 grams per litre of water
Benzyl amino purine (BAP or BA) 5 milligrams per litre (best to buy a preparation as it's a pig to dissolve)
MS medium 2.1 grams per litre (this is half the strength than normal) this can be bought from a laboratory supply centre, I know of 1 in England, which may deliver to Belgium, don't see why not)
pH 5.8

This is the basic medium for initiation and propagation

Plants from test tubes is a useful book although it doesn't mention bananas and goes into setting up a commercial TC lab, which isn't helpful.

Re: looks so simple
 

It is relatively simple if you know what you are doing, but it takes a lot of practice to do everything right. I believe you could learn how to do it just by reading, but it is exponentially easier to learn it by someone showing and explaining to you each step of the process. There are many small things which are very important, but never mentioned in the books because they are difficult to explain with text.

Re: looks so simple
 

there are numerous videos on Youtube that detail many TC procedures.

Sterilization of explants, cleanliness, basic setup etc.

check this guy:

YouTube - Orchid Flasking - Part 1 (2011)

He has like 24 vids up, and I'm pretty sure he is a member here as well. Frank Tromble.

Re: looks so simple
 

What do you mean with
This is the basic medium for initiation and propagation

What plants should I successfully "raise" in this composition?
nana's? haha.

greetz

Re: looks so simple
 

have seen almost all the videos.
tc seems easy if you follow all the points and regulations.
Yet for someone Dutch daily talk, no chemistry background and there is little to be found in my language is not easy.
'm going to try to make a success story out of it.
my goal
gather as much information as possible
English names decipher;-)
right mix of media to find out
and the ultimate goal of making puppies of various plants. musa on top

Re: looks so simple
 

this looks easy for a newby

MUSA (BANANA) MULTIPLICATION MEDIUM - Media, Basal Salts, & Vitamins - M462

hope hazmat,duties, taxes, fees and permits required are not to expensive.

Re: looks so simple
 

I looked at that company and their carriage charge to Europe is a joke! Honestly, if you make a mess of the TC at first then it's a very expensive mistake. I can give names of companies that sell your chemicals, but don't charge stupid amounts of money for carriage, you just have to do a bit of mixing and measuring.

Re: looks so simple
 

sounds like a good proposal.
Of course it is not intended to make lots of money to spend.
it's a hobby and must remain affordable.

Greetz

Re: looks so simple
 

https://micro-science.co.uk/plant-ti...-mixtures.html
https://micro-science.co.uk/plant-ti.../hormones.html
https://micro-science.co.uk/plant-ti...lture/ppm.html

If you have baby food jars I have some of the lids which fit, I can send you some if you like.

Agar can be bought from health food shops or online, lab grade agar is expensive though, but food agar is fine.

Re: looks so simple
 

Thank you very much.
the composition that you have given me,
for which plants you've already used it?
Think if you really want to start from zero to hero an orchid that is reasonable to do.
no complicated incisions.
only node in the mixture and wait for growth.
've seen online that this is a flower that is much used for tc
ready mixes are readily available I think.
easier than for example anannas or banana.
My journey continues.
when there is an end to it is difficult to predict :08:
To boldly go where no man has gone before."

Re: looks so simple
 

One of the most helpful and informative papers i have found on low cost TC is this one. It has a table towards the end mentioning the full composition of the growing media(which is based on Murashige and Skooge basal salts recipe)of the convectional recipe and of one using more ordinary and supposedly easier to find materials. For you and me though,buying the salts ready from a shop like the one James suggested,is the easier and maybe even cheaper solution than finding all of the components and buying them separately. However,the low cost technics mentioned for doing the TC in this paper,are very helpful. Basically the most worrisome part are the sterilization and clean transfers to growing media which require some labor in advance,to prepare a clean area and a low pathogen count place to do the transfer,to achieve good results.
From my search on that so far,the easiest solution to that is making a glove box.

Re: looks so simple
 

thanks kostas:goteam:

Re: looks so simple
 

Has anyone some experience with
Phytorchid.
Seems reasonable prices and no expensive shipping within the EU.

greetz

Re: looks so simple
 

so, the first products arrived with me. yippee
I can soon begin.
bit set in the attic and ready.
do have some questions like,
what kind of light should I use for the tc plants.
Could this in the summer just in daylight?
There will certainly be more questions in my mind and am very happy that so many people want to help me with this.

Thanks guys

Re: looks so simple
 

Indirect daylight is fine and preferable to artificial lighting as it makes for more robust plantlets that need less hardening afterwards,especially if coupled with a air permeable but microbe exclusive, lid.
For absence of daylight,plant specific or "daylight" fluorescent tubes can be used.