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Banana Seed Germination Forum As one of the toughest seeds in the plant kingdom to figure out the keys to germination success with, this is a forum with banana seed germination tips. Please entitle posts like "Musa balbisiana," or "Musa cheesmani," etc. People would then post a reply under that heading, sharing their germination successes (and failures), what materials and methods they used, germination percentage, etc.


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Old 01-30-2009, 04:05 AM   #41 (permalink)
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Default Re: Germination the easy way

If you have any info on that Scot, atricles of some kind. On the Silvernitrate sterilisation,...I would be very interested. Neder heared of people using that for sterilisation.
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Old 01-30-2009, 04:16 AM   #42 (permalink)
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Default Re: Germination the easy way

I have one E. perrieri beginning to grow on 1/2 strength MS with 1Ál/L BA. It's too early to tell if its going to germinate normally or grow callus, I have had both happen depending on the species and medium. If they germinate fairly normally, then they are easy to deal with but if they grow callus then it can be hard to regenerate plants.
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Old 01-30-2009, 12:39 PM   #43 (permalink)
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Default Re: Germination the easy way

Quote:
Originally Posted by griphuz View Post
If you have any info on that Scot, atricles of some kind. On the Silvernitrate sterilisation,...I would be very interested. Neder heared of people using that for sterilisation.
Kind regards,
Remko.
Remko, send a PM to me with your email and I will attache the research article in my reply. Being a heavy metal salt, special disposal laws may apply, so be cautious before you use it.

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I have one E. perrieri beginning to grow on 1/2 strength MS with 1Ál/L BA. It's too early to tell if its going to germinate normally or grow callus, I have had both happen depending on the species and medium. If they germinate fairly normally, then they are easy to deal with but if they grow callus then it can be hard to regenerate plants.
Gabe, that's great news. Was that 1 microliter/liter or 1 micromole/liter. Just being sure. I think a few of the surviving, uncontaminated E. perrieri are showing signs of growth, but not much!

I may take some photos of contaminated cultures to post so everyone can see what it looks like. Some of them are unique patterns.
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Old 01-30-2009, 01:07 PM   #44 (permalink)
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Default Re: Germination the easy way

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Gabe, that's great news. Was that 1 microliter/liter or 1 micromole/liter. Just being sure. I think a few of the surviving, uncontaminated E. perrieri are showing signs of growth, but not much!
1 microliter per liter.

In our lab, we use 6 different mediums. Depending on the variety, they will get either 1/2 strength MS (which is standard) or full strength MS (if they are too slow in the 1/2).Within those, we use either 5Ál/L of BA for shoot multiplication, 1 Ál/L for shoot elongation, and 0Ál/L for rooting.

For embryo rescue, I haven't done enough experiments but have done enough to see that each species responds differently in different media. Sometimes the 5Ál/L was too much and they multiplied like crazy with no way of regenerating plants easily, and sometimes the 0Ál/L did nothing. So I chose the 1Ál/L because it is not extreme like the 5Ál/L but still has a little bit in there to help induce some growth hopefully.
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Old 02-02-2009, 12:22 AM   #45 (permalink)
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Default Re: Germination the easy way

Quote:
Originally Posted by Gabe15 View Post
I have one E. perrieri beginning to grow on 1/2 strength MS with 1Ál/L BA. It's too early to tell if its going to germinate normally or grow callus, I have had both happen depending on the species and medium. If they germinate fairly normally, then they are easy to deal with but if they grow callus then it can be hard to regenerate plants.
It looks like its germinating normally right now. I will transfer it to a medium with no BA in it tomorrow, I don't want to mess with it anymore hormonally while its germinating. After it gains some size in the vessel, I will subculture it to produce backups before regenerating any to plant out.
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Old 02-03-2009, 08:15 PM   #46 (permalink)
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Default Re: Germination the easy way

Still nothing to report on the kinetin/ethrel formula. The seeds are still sitting there but not germinating as yet.
The embryo rescue cultures continue, but progress is slow. Frustrating, but I press onwards.
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Old 02-23-2009, 01:47 AM   #47 (permalink)
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Default Re: Germination the easy way

updated photo of the E. perrieri, its a little slow but appears to be growing fine.

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Old 02-23-2009, 01:48 AM   #48 (permalink)
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Default Re: Germination the easy way

I am green with envy! Nice job Gabe, it looks great! Wish I could get mine to do something.
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Old 02-23-2009, 02:20 AM   #49 (permalink)
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Default Re: Germination the easy way

I didn't do anything special, and for every successful embryo rescue I've had there have been about 15 more that didn't work! So just keep trying, eventually you will be rewarded.
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