griphuz

If you have any info on that Scot, atricles of some kind. On the Silvernitrate sterilisation,...I would be very interested. Neder heared of people using that for sterilisation.
Kind regards,
Remko.

Gabe15

I have one E. perrieri beginning to grow on 1/2 strength MS with 1µl/L BA. It's too early to tell if its going to germinate normally or grow callus, I have had both happen depending on the species and medium. If they germinate fairly normally, then they are easy to deal with but if they grow callus then it can be hard to regenerate plants.

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The only hemp Im growing is Manila.

Chironex

Remko, send a PM to me with your email and I will attache the research article in my reply. Being a heavy metal salt, special disposal laws may apply, so be cautious before you use it.

Gabe, that's great news. Was that 1 microliter/liter or 1 micromole/liter. Just being sure. I think a few of the surviving, uncontaminated E. perrieri are showing signs of growth, but not much!

I may take some photos of contaminated cultures to post so everyone can see what it looks like. Some of them are unique patterns.

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Scot


"Grow 'em if ya got 'em!"

Gabe15

1 microliter per liter.

In our lab, we use 6 different mediums. Depending on the variety, they will get either 1/2 strength MS (which is standard) or full strength MS (if they are too slow in the 1/2).Within those, we use either 5µl/L of BA for shoot multiplication, 1 µl/L for shoot elongation, and 0µl/L for rooting.

For embryo rescue, I haven't done enough experiments but have done enough to see that each species responds differently in different media. Sometimes the 5µl/L was too much and they multiplied like crazy with no way of regenerating plants easily, and sometimes the 0µl/L did nothing. So I chose the 1µl/L because it is not extreme like the 5µl/L but still has a little bit in there to help induce some growth hopefully.

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The only hemp Im growing is Manila.

Gabe15

It looks like its germinating normally right now. I will transfer it to a medium with no BA in it tomorrow, I don't want to mess with it anymore hormonally while its germinating. After it gains some size in the vessel, I will subculture it to produce backups before regenerating any to plant out.

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The only hemp Im growing is Manila.

Chironex

Still nothing to report on the kinetin/ethrel formula. The seeds are still sitting there but not germinating as yet.
The embryo rescue cultures continue, but progress is slow. Frustrating, but I press onwards.

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Scot


"Grow 'em if ya got 'em!"

Gabe15

updated photo of the E. perrieri, its a little slow but appears to be growing fine.

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The only hemp Im growing is Manila.

Chironex

I am green with envy! Nice job Gabe, it looks great! Wish I could get mine to do something.

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Scot


"Grow 'em if ya got 'em!"

Gabe15

I didn't do anything special, and for every successful embryo rescue I've had there have been about 15 more that didn't work! So just keep trying, eventually you will be rewarded.

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The only hemp Im growing is Manila.