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-   -   American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962) (http://www.bananas.org/f30/american-journal-botany-vol-49-no-2725.html)

BGreen 10-13-2007 07:56 PM

American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
Has anyone read the article "Seed Germination Studies in Musa. I. carification and Aseptic Germination of Musa balbisiana" by G. Stotzky, Elsie A. Cox, Roger D. Goos in the American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962), pp. 515-520.

Heres the abstract:
Quote:

Methods for germinating seeds of Musa balbisiana Colla under aseptic conditions have been developed. Scarification was required for germination under these conditions, and mechanical was superior to chemical scarification. Various methods of mechanical scarification and cultural procedures were developed which facilitated the operation when large numbers of aseptic seedlings were required. Removing a chip from the lateral portion of the seed coat to expose the endosperm was the most effective method of scarification: germination percentages averaged 80%, and the time required for germination in sterile culture was shortened from the 3-6 weeks required for intact seeds in soil to 6-10 days. However, scarification did not shorten the time required for germination in soil, and seeds treated with some methods of mechanical scarification failed to germinate, as a result of their decomposition by microorganisms. The effectiveness of scarification in causing germination in aseptic culture is not presently understood, but, as the excised embryo exhibits no dormancy, the factors delaying germination apparently reside in other portions of the seed.

taroking 10-14-2007 10:54 AM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
2 Attachment(s)
I have attached a full copy of it and one other that may be of interest
I hope it worked
Taorking

BGreen 10-14-2007 03:10 PM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
Thank you!
That beats having to go to the college and look it up there!

Chironex 06-12-2008 11:11 AM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
Ben, I have read and re-read this paper and feel that the findings are very significant. It is amazing that more people here have not read this! There is so much here that answers many germination issues. Thanks for making me aware of it. I have adjusted my heat mat to the optimal high of 80-90 degrees F and the timer for 7 1/2 hours on and 16 1/2 hours off. Trying to find a location in my apartment to approximate the ideal low temp of 64-65 degrees.
It is also interesting to note that using a longer time of heat exposure beyond 9 hours, reduces germination percentages! Many people suggest 10-12 hours on and 12-14 off - which was my previous setting. In fact maintained temps over 95 degrees F may actually injure the embryo! They did find that an initial induction for 3 days of alternating between 95 degrees and 65 degrees, followed by reduction to 89 - 65 seemed to increase germination percentages a bit. I think everyone should read this.

damaclese 06-12-2008 11:20 AM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
well as you and i have already discussed on are thread on high temp growing remember the accidental exposure to high temp water that my Ventricosum received and how the first one germinated in just 4.5 days to date no more have germinated so it was probably a fluke!

Chironex 06-12-2008 11:25 AM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
Pauly, I forgot about that, good point. They say that it causes a reduction in germination.Perhaps with the cooling at night some germinated. I meant to ask, have any more germinated?

taroking 06-15-2008 11:23 AM

Re: American Journal of Botany, Vol. 49, No. 5 (May - Jun., 1962)
 
I have experimented with banana seed germination for a few years now. What I did was to make three small germination chambers with heaters in them and placed them in a cool room. The chambers are on a timer, so I can fluctuate the times on high and low. The low temps are somewhat dependent on the weather, as its a passive cool room (like a wine cellar). I think there are temp. triggers for some sp. This may be cool temp triggers during the germination process, as Ive tried many different warm temps and combinations. Some trends have started to emerge with some sp. but some refuse to germinate at any temp regime. I thought of a temp gradient table, with cooling and warming, but they cost about 4K!!! I thought about making a rudimentary one, but the cooling part is a bit complicated. Any ideas?
Pat


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