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| Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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12-19-2009, 04:59 PM
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Working with 10 liter MS packets
Hi, everybody. I should FINALLY be receiving my supplies this coming week.
Would I have to prepare the ten liters as soon as I open one packet or can I store the rest in a ziplock bag or other? According to " Plant propagation by tissue culture " ( Thanks, Remko ), " Autoclaving changes the pH of media.. without sugars the change is small Most agars cause the pH of media to increase immmediately after dissolving... The pH of stored media tend to fall if they are stored... To minimize a change... should be stored in dark at 4ºC... So, could I just prepare the mix of MS ( 2 liters X 5 strength ), without sugar nor PGRs, autoclave and store in the fridge until use? Suggestions appreciated. |
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12-19-2009, 09:10 PM
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Re: Working with 10 liter MS packets
I have stored it in solution in a refrigerator before, but still always had to adjust the pH after adding in the sugar, gelling agent and PGRs. When I did it at home, I bought 1 liter packets and just made 1 liter at a time without adjusting pH, but I also used very purified, non-mineral containing, water (Penta brand) so as to minimize any changes to the medium.
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Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties. |
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12-19-2009, 10:38 PM
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Re: Working with 10 liter MS packets
Quote:
in my lab i make media 10 liter (for 300-350 bottle) every time. is for 2-3 days. depends of your necesssity, but for beginner maybe better only make 1 liter (30-35 bottle). the rest powder MS keep in refrigetator. based on my experienced, agar do not changes pH. its only change 0,01. so thats no problem. but i always do pH every time i make media. i usually make media with pH 5,83. Ms instant media from such company usually do not containing hormone like BAP, so dont forget add it. in my lab i usually mix self all nutrition including makro, mikro, vitamin, hormon etc.
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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12-29-2009, 11:20 PM
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Re: Working with 10 liter MS packets
alah i have here a video of 1 liter of water and i have put a bottle of
MUSA (BANANA) MULTIPLICATION MEDIUM that makes (1 liter) media |
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12-29-2009, 11:48 PM
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#5 (permalink) |
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Tissue Culturist
Location: Jakarta, Indonesia
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Re: Working with 10 liter MS packets
make video is very appreciate Fernie. lets all share our knowledge about TC bananas
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Novi Syatria Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com |
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