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-   -   Working with 10 liter MS packets (http://www.bananas.org/f260/working-10-liter-ms-packets-10671.html)

alah 12-19-2009 04:59 PM

Working with 10 liter MS packets
 
Hi, everybody. I should FINALLY be receiving my supplies this coming week.
Would I have to prepare the ten liters as soon as I open one packet or can I store the rest in a ziplock bag or other?

According to " Plant propagation by tissue culture " ( Thanks, Remko ),
" Autoclaving changes the pH of media.. without sugars the change is small
Most agars cause the pH of media to increase immmediately after dissolving...
The pH of stored media tend to fall if they are stored...
To minimize a change... should be stored in dark at 4ºC...

So, could I just prepare the mix of MS ( 2 liters X 5 strength ), without sugar nor PGRs, autoclave and store in the fridge until use?

Suggestions appreciated.

Gabe15 12-19-2009 09:10 PM

Re: Working with 10 liter MS packets
 
I have stored it in solution in a refrigerator before, but still always had to adjust the pH after adding in the sugar, gelling agent and PGRs. When I did it at home, I bought 1 liter packets and just made 1 liter at a time without adjusting pH, but I also used very purified, non-mineral containing, water (Penta brand) so as to minimize any changes to the medium.

novisyatria 12-19-2009 10:38 PM

Re: Working with 10 liter MS packets
 
Quote:

Originally Posted by alah (Post 114893)
Hi, everybody. I should FINALLY be receiving my supplies this coming week.
Would I have to prepare the ten liters as soon as I open one packet or can I store the rest in a ziplock bag or other?

According to " Plant propagation by tissue culture " ( Thanks, Remko ),
" Autoclaving changes the pH of media.. without sugars the change is small
Most agars cause the pH of media to increase immmediately after dissolving...
The pH of stored media tend to fall if they are stored...
To minimize a change... should be stored in dark at 4ºC...

So, could I just prepare the mix of MS ( 2 liters X 5 strength ), without sugar nor PGRs, autoclave and store in the fridge until use?

Suggestions appreciated.

congartulation Alberto
in my lab i make media 10 liter (for 300-350 bottle) every time. is for 2-3 days.
depends of your necesssity, but for beginner maybe better only make 1 liter (30-35 bottle). the rest powder MS keep in refrigetator. based on my experienced, agar do not changes pH. its only change 0,01. so thats no problem. but i always do pH every time i make media.
i usually make media with pH 5,83.
Ms instant media from such company usually do not containing hormone like BAP, so dont forget add it. in my lab i usually mix self all nutrition including makro, mikro, vitamin, hormon etc.

xavierdlc61887 12-29-2009 11:20 PM

Re: Working with 10 liter MS packets
 
alah i have here a video of 1 liter of water and i have put a bottle of
MUSA (BANANA) MULTIPLICATION MEDIUM that makes (1 liter) media



i put it in the stirrer for about 10 minutes...let all the powder disolve and get mixed in with the water. then i put 20ml of media into some jars and autoclaved them for 15 minutes and here is the result of one of the jars


novisyatria 12-29-2009 11:48 PM

Re: Working with 10 liter MS packets
 
make video is very appreciate Fernie. lets all share our knowledge about TC bananas


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