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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 07-17-2009, 08:46 AM   #1 (permalink)
 
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Default Virus?

I have read all the conversations here and have greatly enjoyed it. I want to ask if anyone else here is endeavouring to insert a "color break" virus into clean banana plants.My efforts (unsucessful)have been to pulp Ae Ae tissue into the base of the corm of other non-vartiegated bananas.My premise is --When Agri-Starts mericloned Ae AE --the plants were NOT variegated. To me this indicated the color break was viral in origin . If true THEN why not inject Ae Ae pulp into the base of the corm of --for instance-- a Basjoo plant and produce variegated Basjoo??? So far NO success But perha
ps worth more effort.Don Hudson
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Old 07-17-2009, 10:15 AM   #2 (permalink)
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Default Re: Virus?

Quote:
Originally Posted by don8t1 View Post
I have read all the conversations here and have greatly enjoyed it. I want to ask if anyone else here is endeavouring to insert a "color break" virus into clean banana plants.My efforts (unsucessful)have been to pulp Ae Ae tissue into the base of the corm of other non-vartiegated bananas.My premise is --When Agri-Starts mericloned Ae AE --the plants were NOT variegated. To me this indicated the color break was viral in origin . If true THEN why not inject Ae Ae pulp into the base of the corm of --for instance-- a Basjoo plant and produce variegated Basjoo??? So far NO success But perha
ps worth more effort.Don Hudson
Just go on, but know, that variegation is genetic defect, not a viral infection.
Do keep us posted on your progress.
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Thnx to Marcel, Ante, Dr. Chiranjit Parmar and Francesco for the plants I've received.



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Old 07-17-2009, 12:28 PM   #3 (permalink)
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Default Re: Virus?

Some variegations are infact caused by viruses in the plant, but in the case Aeae it is not viral, but a somatic mutation that has taken place that causes the plant to become chimeric. The reason it cannot be successfully mass tissue cultured (there has been a few successes, but its still easier to grow from pups) is because the variegated tissue is spread across the meristem in such a manner that when you take a small piece of tissue to culture, it is very difficult to get all of the different chimeric tissue colors in one small piece that will continue to grow together.

Agri-Starts did infact produce 7 stable variegated Aeae from tissue culture over the course of 4 years, that of course was not economically feasible at all so they gave up.

I also know of some successful tissue cultured Aeae that stayed stable for 4 generations only before reverting to green.
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