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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 01-21-2013, 08:53 PM   #1 (permalink)
 
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Default Trying to germinate usa veluntina in TC

Hello everyone i am trying to germinate Musa Veluntina in tissue culture and would like to know if anyone has a metho they could share.
Currently i have my seeds in a 1/2 strength Murashige and Skoog Media solidified with gellan gum. There is 3PPM gibberellic acid in the media as well as .1% Plant preservative mixture. and 1% sucrose

If you see any problems with my current set up could you let me know

Last edited by klauts : 01-21-2013 at 08:55 PM.
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Old 01-22-2013, 02:43 AM   #2 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

gibberillic acid inhibits germination of banana seeds
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Old 01-22-2013, 07:41 PM   #3 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

that is not true at all at least not when Musa Veluntina is the banana in question

This study claims other wise

and if you would like me to dig them up again i read three other studies claiming gibberellic acid helped germination of musa veluntina as well... I have not found a single study suggesting gibberellic acid inhibits germination Of Musa Veluntina and if you please provide me a link to one that would be useful as i would like to see what the conditions were besides the application of Ga3
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Old 01-23-2013, 03:00 AM   #4 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

Quote:
Originally Posted by klauts View Post
that is not true at all at least not when Musa Veluntina is the banana in question

This study claims other wise

and if you would like me to dig them up again i read three other studies claiming gibberellic acid helped germination of musa veluntina as well... I have not found a single study suggesting gibberellic acid inhibits germination Of Musa Veluntina and if you please provide me a link to one that would be useful as i would like to see what the conditions were besides the application of Ga3
If I had the resources that you clearly do for obtaining scientific papers then I would show you as many papers that say gibberillic acid has no effect on germination and actively inhibits germination. I can get velutina seeds ot germinate without using aseptic techniques, so clearly that paper is based on a rubbish summation.

If gibberillic acid has an effect on germination why are you asking for help? Why haven't your seeds germinated yet? Why are you being so defensive when someone has a different opinion to you? If you want or need information then I suggest you find it in your journals and don't be rude to members here who have a different opinion to yours.
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Old 01-23-2013, 08:53 AM   #5 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

Use whatever works best for you
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Old 01-23-2013, 05:49 PM   #6 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

For anyone wanting to germinate "musa velutina," it is simple as using jiffy trays, seed starting soil, and a Ziploc bag. It takes approximately two weeks but this was the method that I used. I had a 63.6% (7 out of 11) germination rate. I do not know if this would work for other varieties of banana plants to start seeds as I have heard they have been more difficult to germinate.
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Old 01-23-2013, 06:11 PM   #7 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

Quote:
Originally Posted by jmoore View Post
If I had the resources that you clearly do for obtaining scientific papers then I would show you as many papers that say gibberillic acid has no effect on germination and actively inhibits germination. I can get velutina seeds ot germinate without using aseptic techniques, so clearly that paper is based on a rubbish summation.

If gibberillic acid has an effect on germination why are you asking for help? Why haven't your seeds germinated yet? Why are you being so defensive when someone has a different opinion to you? If you want or need information then I suggest you find it in your journals and don't be rude to members here who have a different opinion to yours.
i was just looking to see how other people did it and to get to know the community and i asked if u could provide me with the references not because i was being defensive but because i actually wanted to see the information to better educate myself and i only said gibberellic acid doesnt inhibit musa germination i didnt say anything at all about other types of banana's... obviously i should just stick to reading and not bother askng questions thanks for the clarification
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Old 01-24-2013, 02:56 AM   #8 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

Sorry you caught me at a bad moment, I misinterpreted the post. I have read it somewhere, just remembering where is the challenge.
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Old 01-24-2013, 08:22 AM   #9 (permalink)
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Default Re: Trying to germinate usa veluntina in TC

Klaus did you put the seeds in the medium as a whole of did you get the embryo's out (sterile)?

Kind regards,
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Old 01-27-2013, 02:56 PM   #10 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

Hey thanks everyone sorry i got so snappy there just got offended a little to easily... anyways the seeds were put into the microplates whole after being soaked in warm water for 3 days. I'm starting to gather that it is mandatory to scarify the seed... does anyone have a good method for doing so? Also how would you go about extracting the embryo without damaging it??


P.s. the seeds were sterilized for 4 hours in a 5% Plant Preservative Mixture solution. prior to being placed in the medium

Last edited by klauts : 01-27-2013 at 03:05 PM.
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Old 01-28-2013, 01:51 AM   #11 (permalink)
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Default Re: Trying to germinate usa veluntina in TC

hello,

here is a doc about GERMINATION OF Musa balbisiana SEEDS AND EMBRYOS (both english & french)

i think that's exactly what you are doing with velutinas.

but they don't explain how they cut the seeds longitudinally.
(nail clippers ?)
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Old 01-28-2013, 02:46 AM   #12 (permalink)
 
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Default Re: Trying to germinate usa veluntina in TC

When trying thsi technique I used a pen knife, you need something sturdy to get through the seed coat. I've also read the embryo being squeezed out like a spot with a pair of pliers, that was with heliconia though.
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