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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 08-10-2008, 11:31 AM   #1 (permalink)
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Default Tissue culture in the UK

I am very intersted in this tissue culture and i have read the threads on here and i have to agree there is not that much info on the internet, gabe provided a wealth of information tho, but i do not understand when do you quarter them as in gabes diagram i noticed that it shows keep quartering them so that you get many plants fromt he one source but at what point do you quarter them. Also i am intersted to hear from anyone in the uk who has treid tc in the UK and where they got the items needed from here in the uk as the kits that are avaliable are from the US and shipping works out quite expensive.
Is it right if you micropro or tc a banana then you basically end up with clones of that one plant and they will all do the same, ie flower at same time etc
Thanks in advance Mark
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Old 04-27-2009, 03:31 PM   #2 (permalink)
 
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Default Re: Tissue culture in the UK

Hi have a look at - Applied Microscience Ltd i have not had a go with tissue culture but would like to give it a go i just need to find the time..
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Old 04-28-2009, 12:42 PM   #3 (permalink)
 
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Default Re: Tissue culture in the UK

I too would like to have a go, but I'm busy busy busy! Also the lights worry me a bit, the thought of getting my front door broken down by plod at six o'clock in the morning worries me a bit only for me to say "I'm growing nanas, officer." May be a bit embarrassing all round.
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Old 04-28-2009, 01:01 PM   #4 (permalink)
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Default Re: Tissue culture in the UK

Hehe, embarrassing garanteed recepie.
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Old 04-29-2009, 03:48 AM   #5 (permalink)
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Default Re: Tissue culture in the UK

Quote:
Originally Posted by marksbananas View Post
I am very intersted in this tissue culture and i have read the threads on here and i have to agree there is not that much info on the internet, gabe provided a wealth of information tho, but i do not understand when do you quarter them as in gabes diagram i noticed that it shows keep quartering them so that you get many plants fromt he one source but at what point do you quarter them. Also i am intersted to hear from anyone in the uk who has treid tc in the UK and where they got the items needed from here in the uk as the kits that are avaliable are from the US and shipping works out quite expensive.
Is it right if you micropro or tc a banana then you basically end up with clones of that one plant and they will all do the same, ie flower at same time etc
Thanks in advance Mark
They usually need at least 1 month of growth between subcultures (quartering), but if you can't get around to it right away they can hang out for awhile without much consequence.

What I tell people who ask about how to tissue culture bananas is that they need to do their own research about tissue culture in general and make sure they are well familiar with all the basics and what it actually involves and the theory behind it. Once that is all understood I can help to how to tissue culture bananas specifically, but it's very difficult and confusing to explain the process of tissue culture of bananas when people are only interested in getting more banana plants without understanding what is involved in the basic process with any plant in tissue culture. Its sort of like I could give you some pointers on how to drive a specific car model, but if you don't know how to drive any car, then anything I tell you is pretty meaningless until you know the basics of driving cars in general.
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Old 08-28-2009, 03:32 AM   #6 (permalink)
 
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Default Re: Tissue culture in the UK

This place seems to sell lots of items needed for TC:

Looking for Tissue Culture Consumables?
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Old 09-09-2009, 04:57 PM   #7 (permalink)
 
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Default Re: Tissue culture in the UK

Is this correct, Cabe15?
Quartering or dividing your explant is subculturing. the two main reasons for this are: first the nutrients in the media are consumed; someday, nothing left to eat! Secondly, metabolism generates waste products and buildup or accumulation willbecome toxic.
So I assume that a culture with 500cc of medium could stand alone for more time than the same explant with only 100cc of media.
the problem is HOW to recognize or calculate when this is going to happen!







Quote:
Originally Posted by Gabe15 View Post
They usually need at least 1 month of growth between subcultures (quartering), but if you can't get around to it right away they can hang out for awhile without much consequence.

What I tell people who ask about how to tissue culture bananas is that they need to do their own research about tissue culture in general and make sure they are well familiar with all the basics and what it actually involves and the theory behind it. Once that is all understood I can help to how to tissue culture bananas specifically, but it's very difficult and confusing to explain the process of tissue culture of bananas when people are only interested in getting more banana plants without understanding what is involved in the basic process with any plant in tissue culture. Its sort of like I could give you some pointers on how to drive a specific car model, but if you don't know how to drive any car, then anything I tell you is pretty meaningless until you know the basics of driving cars in general.
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