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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 12-30-2009, 06:11 AM   #1 (permalink)
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I think its amazing see alot of baby pineapple in one bottle. i have alot bottle pineapple and this is for sample. is it awesome right?
Banana Gallery - IMGP2575_Pineapple_1
Banana Gallery - IMGP2572_Pine_apple_2
Banana Gallery - IMGP2573_Pine_apple_3

kind regards from Jakarta

i forget to explain that pine apple developed from shoot crown. its the best for pineapple tc
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Old 01-03-2010, 07:41 PM   #2 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi novisyatria,

I have a Pineapple crown going in a pot. Can I use that for an explant?
Would I use sections of the of the base of the crown, kind of like you would for a banana? I would really like to try this.

Thanks!
Frank
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Old 01-03-2010, 07:57 PM   #3 (permalink)
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Default Re: Tissue Culture Pineapple

Hi Frankco
yes you can use the crown as explant. its very good as explant source. take the shoot (part tip crown). for sterization using bleach 10 % ( 10 ml bleach + 90 ml sterile water) for 10 minute, rinse 5 x with sterile water, and then soak in alkohol 70 % for 3 minute. rise 5 5x with water. and explant ready to planted. try media MS + 2 mg BAP + 5 mg NAA for shoot multification.
good luck Frank. dont forget post your progress
regards
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Old 01-03-2010, 08:10 PM   #4 (permalink)
 
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Default Re: Tissue Culture Pineapple

Thanks novisyatria! I'm going to try it this next weekend. I'll post picts of the before and after:-)

I am amazed at your caladium too. I have one of those growing too. I may just give that a try also.

Thanks so much for the suggestion of disinfecting and the media. I'm really excited to give it a try!
Frank
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Old 01-03-2010, 08:42 PM   #5 (permalink)
 
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Default Re: Tissue Culture Pineapple

May I ask what gelling agent you use. It looks nice and clear.

Thank you,
Frank
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Old 01-03-2010, 08:50 PM   #6 (permalink)
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Default Re: Tissue Culture Pineapple

i use agar-agar that we usually use for puding. alot in supermarket and cheap heheh. 1 sachet containing netto 7 gr. its enough for 1 liter media.
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Old 01-20-2010, 03:21 AM   #7 (permalink)
 
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Default Re: Tissue Culture Pineapple

novisyatria will u post an image of pineapple(part tip crown) for the example
thanks im ur Ym buddy..
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Old 01-21-2010, 08:18 AM   #8 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi novisyatria,

I didn't get around to trying the pineapple yet. I've been busy with other plants, mainly bananas.

I did a test with Gelcarin GP812 on bananas to see if it would help with the problem of phenolic extrudes. So far it seems to being working quite well. I was having problems with about 1 out of 3 cultures. Now, none are showing the problem.
Gelcarin is hard to get. It's actually cheaper than agar, $38 a kilo, if bought in bulk but, everyone jacks the price up to make it more expensive.

One nice thing about Gelcarin is that you don't have to heat it to make a gel. That saves some time. You do have to autoclave it to sterilize it though but no double heating. You can just add 7 grams in water; the stuff dissolves and turns crystal clear. Lovely stuff.

I do want to try pineapple, maybe this weekend. When I do, I'll post some picts of the explant I use. I'm not sure, since I have the crown growing in a pot of soil, whether contamination might have gotten inside of the plant. If so, I'll go out and buy another pineapple from the store and use that.

After I post the pict maybe I could get your opinion on how my explant looks.

Thanks,
Frank
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Old 01-21-2010, 07:20 PM   #9 (permalink)
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Default Re: Tissue Culture Pineapple

greeting Frank. i will wait your pos about pineapple. i dont know about gelcarin but if good you can use it. can you show me your media?
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Old 01-21-2010, 07:43 PM   #10 (permalink)
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Default Re: Tissue Culture Pineapple

Novi, just a question. Seeing how much you work in TC area, what are your average monthly costs in $. For everything... media, pots, lab, initial investitions... I kinda wonder, how expensive it really is in your area. Thanks.
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Old 01-21-2010, 07:46 PM   #11 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi novisyatria,

Here are a couple of picts of plants in Gelcarin. The first one is a banana of course. The second one is a Drosera Venus Flytrap. I put food coloring in the media so I can tell them appart.



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Old 01-21-2010, 10:20 PM   #12 (permalink)
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Quote:
Originally Posted by Jack Daw View Post
Novi, just a question. Seeing how much you work in TC area, what are your average monthly costs in $. For everything... media, pots, lab, initial investitions... I kinda wonder, how expensive it really is in your area. Thanks.
maybe the cost is 150 - 200 $ a month.

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Originally Posted by Frankco View Post
Hi novisyatria,

Here are a couple of picts of plants in Gelcarin. The first one is a banana of course. The second one is a Drosera Venus Flytrap. I put food coloring in the media so I can tell them appart.



you add food coloring/ oh no. food colors is poison for explant. your explant will die.

rgrds
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Old 01-21-2010, 10:55 PM   #13 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi novisyatria,

No, food coloring isn't poisonous to the plants. I have about 350 Musa Basjoo explants in jars and another 100 jars filled with different plants that all have food coloring in them. They are doing fine.


very light blue coloring

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Old 01-21-2010, 11:58 PM   #14 (permalink)
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Default Re: Tissue Culture Pineapple

are you sure?
i ever try add food colors and the results not good. even there is explant can growth but they multification and proliferation limited. that why all scientist that work with tc never used food colors, except for souvenir for example.
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Old 03-15-2010, 09:21 AM   #15 (permalink)
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Default Re: Tissue Culture Pineapple

Pineapple shoots in media 2 BAP


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Old 03-15-2010, 09:53 AM   #16 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi Novi! They look great!

Nothing is happening with my pineapple yet. I bought a pineapple this weekend and will give this one a try. I'm going to take a video of the process. I think I cut too small of pieces with the last pineapple. I did try to leave some hard base and soft upper section on the explants but maybe cut them too thin. Maybe I should make my cuttings a thicker and a little larger?

How long does it take for them to respond? Do you lay them on their sides on top of the media or poke them in?

Oh, I tried a caladium and a small shoot is just starting to show.
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Old 03-15-2010, 10:20 AM   #17 (permalink)
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Default Re: Tissue Culture Pineapple

Frank
yes i think you should more thicker. for bananas respod to hormone about 3-4 weeks. but after have callus they wll growth faster and can sulcultured soon. congrats for your caladium explant. in what kind media? i did it with 1 mg TDZ. did you used coloring foods for this caladium? maybe better without food colors. i will post my new caldium callus in other thread
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Old 03-15-2010, 10:28 AM   #18 (permalink)
 
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Default Re: Tissue Culture Pineapple

Hi Novi,

I'm good with bananas. I have a bunch of them going.

The pineapple, not yet. I put some of the slices vertically in the media and some I just laid on top. The ones I laid on top seem to be doing better. The ones I put in vertically, the tops have turned dark. Is that normal?

Thanks Novi.

Oh, yes, I did use food coloring. I hope it doesn't hurt them
I'll try them without food coloring.
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Old 03-15-2010, 10:37 AM   #19 (permalink)
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Default Re: Tissue Culture Pineapple

i put them vertically and they all good. i think its efefct food colors. for tc pineapples through callus induction more sensitif than shoot induction directly like bananas. so try with no food color.
part top become dark i think maybe they got browning. maybe you cut too small and tehre some bleach unclean or sterilieze too hard or too long. but my pineapple explant have not turn dark like your.

regards
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Old 03-15-2010, 10:48 AM   #20 (permalink)
 
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Default Re: Tissue Culture Pineapple

Thank you Novi! I will try bigger pieces, no food coloring and watch my disinfecting times.
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