tissue culture jar question

obdiah · 09-06-2017, 04:52 PM

hi I am thinking about trying tc again third time is the charm or so I have heard
last two attempts grew callus tissue and turned green but went no further I used baby food jars and their original lids not vented is it necessary to have vented lids for gas exchange?
their is a possibility I did not get the proper meristem tissue in past attempts

anita · 10-18-2017, 02:20 PM

No, you don't need to vent them.
Was the callus brown after all?
Maybe your PGR was not well dosed.

obdiah · 10-18-2017, 04:45 PM

they formed callus tissue grew larger then turned green they remained green for 2 months
but never formed shoots then turned brown and died the pgr i used was pre mixed purchased from ebay and was probably not the best next attempt I will order from
PhytoTechnology Laboratories I just got my new (used) laminar flow cabinet in place up stairs and geting my work space set up

thanks for your help

anita · 10-19-2017, 02:26 AM

That's a nice piece of equipment. Looks like you are planning big jobs.
You callus didn't grow most likely because of lack of auxins. By the way auxins disintegrate when exposed to light and heat. So you need to change the medium more often.
Did you control the light and temperature?
With the right variables in place you should obtain 3-6 bananans in 3 weeks from the right callus. Then subcultured.

obdiah · 10-19-2017, 05:50 AM

temperature was room temperature in my house around 70 f I did not control lighting
yes this is just a hobby at least for now the flow hood was so cheap I could not say no

$190 us dollars I had been using an aquarium turned on its side
thanks again for your help

09-06-2017, 04:52 PM	#1 (permalink)
obdiah Join Date: Jun 2014 Posts: 307 BananaBucks : 101,379 Feedback: 0 / 0% Said "Thanks" 162 Times Was Thanked 576 Times in 186 Posts Said "Welcome to Bananas" 22 Times	tissue culture jar question hi I am thinking about trying tc again third time is the charm or so I have heard last two attempts grew callus tissue and turned green but went no further I used baby food jars and their original lids not vented is it necessary to have vented lids for gas exchange? their is a possibility I did not get the proper meristem tissue in past attempts

10-18-2017, 02:20 PM	#2 (permalink)
anita Location: Bucharest, Romania Find me on the map! Zone: 7a Join Date: Mar 2016 Posts: 41 BananaBucks : 17,202 Feedback: 0 / 0% Said "Thanks" 8 Times Was Thanked 54 Times in 28 Posts Said "Welcome to Bananas" 0 Times	Re: tissue culture jar question No, you don't need to vent them. Was the callus brown after all? Maybe your PGR was not well dosed.

10-18-2017, 04:45 PM	#3 (permalink)
obdiah Join Date: Jun 2014 Posts: 307 BananaBucks : 101,379 Feedback: 0 / 0% Said "Thanks" 162 Times Was Thanked 576 Times in 186 Posts Said "Welcome to Bananas" 22 Times	Re: tissue culture jar question they formed callus tissue grew larger then turned green they remained green for 2 months but never formed shoots then turned brown and died the pgr i used was pre mixed purchased from ebay and was probably not the best next attempt I will order from PhytoTechnology Laboratories I just got my new (used) laminar flow cabinet in place up stairs and geting my work space set up thanks for your help

10-19-2017, 02:26 AM	#4 (permalink)
anita Location: Bucharest, Romania Find me on the map! Zone: 7a Join Date: Mar 2016 Posts: 41 BananaBucks : 17,202 Feedback: 0 / 0% Said "Thanks" 8 Times Was Thanked 54 Times in 28 Posts Said "Welcome to Bananas" 0 Times	Re: tissue culture jar question That's a nice piece of equipment. Looks like you are planning big jobs. You callus didn't grow most likely because of lack of auxins. By the way auxins disintegrate when exposed to light and heat. So you need to change the medium more often. Did you control the light and temperature? With the right variables in place you should obtain 3-6 bananans in 3 weeks from the right callus. Then subcultured.

10-19-2017, 05:50 AM	#5 (permalink)
obdiah Join Date: Jun 2014 Posts: 307 BananaBucks : 101,379 Feedback: 0 / 0% Said "Thanks" 162 Times Was Thanked 576 Times in 186 Posts Said "Welcome to Bananas" 22 Times	Re: tissue culture jar question temperature was room temperature in my house around 70 f I did not control lighting yes this is just a hobby at least for now the flow hood was so cheap I could not say no $190 us dollars I had been using an aquarium turned on its side thanks again for your help

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