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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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06-06-2008, 10:36 AM | #1 (permalink) |
many 'naners, little time
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Tissue Culture
following on from the Ensete Perrieri thread... Is there sufficient demand from .org members to jointly put some of the more unusual species into TC?
I seem to remember a number did this with Siam Ruby a while back. maybe we could create 'wishlists' and see what level of demand is out there. I know that there are specialists out there doing this but it would be a great way for .org to spread some species out more widely across the globe. any thoughts?
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06-06-2008, 11:02 AM | #2 (permalink) |
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Re: Tissue Culture
Iʻm working on it right now, I have a whole setup in my house ready to go, Iʻm just waiting for my media to come in and hopefully I can start producing some. I already have much of my collection in Hawaii in TC already (including some very unusual species, and many plants not yet available on the market), when I get back there I plan to start selling plants by January hopefully.
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06-06-2008, 11:03 AM | #3 (permalink) |
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Re: Tissue Culture
Kev, I couldn't agree more. I am reading up on TC technique and will be able to do it in a few months or less. I was discussing this with Gabe too. It will definitely help the banana world and I want to be part of that.
Once I get set-up to where I know what I am doing, I will be offering this service to our membership. I am not sure just yet, but I think that taking the tissue from the explant can be done without causing too much injury. From what I have read, tissue is taken from the meristem, which is either growing root tips or in shoots. So, it shouldn't be that invasive as to cause unrecoverable injury to the source plant (explant). Just need to get a comfort level on my own stock first. Then Dr Frankenstein will be ready to party!!!! |
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06-06-2008, 11:51 AM | #4 (permalink) |
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Re: Tissue Culture
Would anyone like to post a good link about tissue culture for bananas. I am not looking to do it but am interested in how it works.
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06-06-2008, 12:38 PM | #5 (permalink) | |
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Re: Tissue Culture
Quote:
Micropropagation of Musa |
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06-06-2008, 01:42 PM | #6 (permalink) |
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Re: Tissue Culture
It turns out that different generas of plants require different kinds of tissue culture. In other words, some plants are more troublesome than others. Here's a paper I came across while researching the origins of some pineapple guava varieties:
ScienceDirect - Scientia Horticulturae : Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): Induction, conversion and synthetic seeds
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06-07-2008, 03:11 AM | #7 (permalink) |
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Re: Tissue Culture
Chong, Thanks for the link. Very interesting reading. I did think there would be more pictures after the meristem was removed showing various stages of growth.
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06-07-2008, 09:07 AM | #8 (permalink) | |
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Re: Tissue Culture
Quote:
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06-07-2008, 09:16 AM | #9 (permalink) |
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Re: Tissue Culture
Pauly, I forgot to mention to you that I have made arrangements for 2 Mysore and have another contact for more if needed. PM me with your address if you want and I can have them sent to you before I get there.
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06-07-2008, 10:08 AM | #10 (permalink) |
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Re: Tissue Culture
I have heard from other Musa (fruiting) growers and read a few articles indicating that Tc of Musa usually produces a new cultivar of the plant and less often a plant that is completely true to form of the parent. What are your experiences with this?
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06-07-2008, 10:50 AM | #11 (permalink) |
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I am not an expert yet, but this contradicts what I have read. The clone produced through micropropagation is an identical copy of the parent (as are all other plantlets multiplied from the same explant.) The only possible way that this can be deviated is through contamination or mutagens deliberately applied to the culture.
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06-07-2008, 11:45 AM | #12 (permalink) | ||
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Re: Tissue Culture
Quote:
Quote:
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06-07-2008, 11:52 AM | #13 (permalink) |
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Re: Tissue Culture
Thanks Gabe for that excellent clarification.
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06-07-2008, 05:39 PM | #14 (permalink) |
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Re: Tissue Culture
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06-07-2008, 07:57 PM | #15 (permalink) | |
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Re: Tissue Culture
Quote:
NOTE: The plants do not actually get smaller and smaller, I just only had a finite working space to show the examples.
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06-07-2008, 08:47 PM | #16 (permalink) |
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Re: Tissue Culture
good luck on this project. hopefully it will make it easier to obtain great plants for our own collections and prevent some of the rarer plants becoming extinct.
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06-07-2008, 08:49 PM | #17 (permalink) |
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Re: Tissue Culture
I received the copy of "Plants From Test Tubes" by Kyte and Kleyn today and started reading it. So far the writing style and information content is excellent.
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06-09-2008, 10:54 PM | #18 (permalink) |
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Re: Tissue Culture
Richard I got the same book from Amazon last week and I'm reading it too. I have also made contact with the Doctor that runs the Kitchen Culture website. She has sent me some more info as well as given my email to her associate who specializes in bananas. I must have downloaded a gazillion pages of info on TC already. Now if i could just find the time to read all of it. Burned through a fresh ink cartridge already.
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06-09-2008, 11:34 PM | #19 (permalink) | |
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Re: Tissue Culture
Quote:
Also I would caution that reading the "how to" of Tiissue Culture is a bit like reading how to be a master cabinet maker. I expect it is going to take me a lot of patience and practice, including going through the amateur, apprentice, and journeyman phases.
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06-10-2008, 06:07 AM | #20 (permalink) |
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Re: Tissue Culture
Yes, I've been on the home tissueculture webgroup for quite some time now >2 years, and I've learned a lot just from what others are posting. It seems the biggest issue is getting the right chemical blend for propagation of particular plants, they do recommend certain chemicals for bananas, but getting them "dialed in" can be a challenge. Also the biggest problem is contamination. Everything has to be sterile and kept that way. Even the smallest particle of dust contains thousands of different contaminants from bacteria to fungus.
I haven't actually tried tc yet because of lack of time issues, maybe this winter. I said that last year too, though. I did get a cloning machine though, that sounded very do-able to me. I'm supposed to get it today.
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