Tissue Culture - Page 2

Chironex · 06-10-2008, 07:29 AM

Quote:

Originally Posted by sandy0225

I said that last year too, though.
I did get a cloning machine though, that sounded very do-able to me. I'm supposed to get it today.

A cloning "machine"? Do you have a link to it? I would be interested to see it. What does it do?

musa_monkey · 06-10-2008, 07:59 AM

This maybe ?

Hydroponics Cloning Machines - Plantlighting Hydroponics & Grow Lights

MediaHound · 06-10-2008, 10:38 AM

Looks like this thread is really blowing wind into some sails!!
I'm elated to see all the interest everyone has into tissue culturing and bringing some of the more rare bananas into production. I have always had an interest in TC'ing but have never taken the initiative. Perhaps watching the success of our members will help convince me to find the time and space and set it up properly.

Quote:

Originally Posted by Gabe15

Iʻll try to find some pictures, but in the meantime, I made up this diagram showing basically what happens after the initiation (when tissue culture becomes micropropagation). You will come across inconsistencies in actual practice, such as dead explants, contamination, varying degrees of proliferation, size of regenerated meristems and duration between meristem regenerations. But its generally what happens, or at least the way I do it is.

NOTE: The plants do not actually get smaller and smaller, I just only had a finite working space to show the examples.

This is great! Thanks for taking the time to create it.
BTW, I copied it over into the banana diagrams category in the gallery.

Thread stuck by the way, everyone, so we can use it as a general reference and a place to point people to.

We do have a page in the wiki about tissue culturing, it's here:
Info:Tissue Culturing - Bananas Wiki
It's fleshing out, but these things take time. Feel free to add to it, we can use both this thread and the page in the wiki as a springboard for information.

Cheers

Dean W. · 06-10-2008, 12:15 PM

I want to keep track of this thread. I have an Ensete perreri that might be TC material.

Chironex · 06-28-2008, 03:06 PM

Dean, keep in mind that TC'ing a banana requires the original pseudostem to be destroyed. We dissect the apical meristem from the base of the pseudostem. You might be able to re-grow from the corm.
There is always a risk of contamination in TC work, however small the percentage (Less than 2% hopefully), so always keep a back-up, just in case. I have several Ensete perrieri seeds in germination, so if I get a decent germination rate, I plan to TC one of them.

Richard · 06-28-2008, 04:24 PM

I thought that banana TC has been successfully initiated from growth tips and cambium material in rhizomes. Is this true and just not a preferred practice, or ?

Chironex · 07-03-2008, 05:57 PM

I thought all might be interested to know that I have been emailing the guy who created Little Prince. It is a sport that was created in TC from 4 mutants. It is patented as a variety, so TC'ing it will require permission. I will invite him too join our forum.

mskitty38583 · 07-04-2008, 01:23 AM

that would be awesome scot!

Chironex · 07-04-2008, 11:43 AM

Update: He just emailed me that he will be joining as soon as he gets his computer back online. Apparently a Microsoft security update has locked him out of his desktops and he is having it repaired.
Bottom line is he will be joining us soon. His name is Randy, but I don't have his last name.
It will be exciting to include him among our membership. It will also be helpful to anyone who develops their own sport and seeks to have it patented, from someone who knows the ropes.

Micronana · 08-07-2008, 04:26 AM

Hi New member here,
The diagram from Gabe 15 was brilliant, I am microing musa basjoo, dwarf cavendish and ensete v. maurelii (although the last 2 are new to me, and only just initiated).
I have never tried being quite so brutal with them, I usually just cut them down the middle. Might try quartering some in future.
By the way, I've recently read that the ensete don't produce pups spontaniously. Mine in culture have already started sprouting! I'll try damaging a few anyway to see the results. Has any one got any experience of them?

Thanks Dawn

Gabe15 · 08-07-2008, 08:35 AM

Quote:

Originally Posted by Micronana

Hi New member here,
The diagram from Gabe 15 was brilliant, I am microing musa basjoo, dwarf cavendish and ensete v. maurelii (although the last 2 are new to me, and only just initiated).
I have never tried being quite so brutal with them, I usually just cut them down the middle. Might try quartering some in future.
By the way, I've recently read that the ensete don't produce pups spontaniously. Mine in culture have already started sprouting! I'll try damaging a few anyway to see the results. Has any one got any experience of them?

Thanks Dawn

Hello and welcome Dawn,
Ensete do not naturally produce pups in the ground, but in culture they will proliferate like normal with the benzyladenine in the media.

51st state · 08-10-2008, 05:15 PM

Hi Dawn
welcome aboard the best banana site in the world!!!!!!!!!!!!!

great to see you trying TC'ing some nana's. If you can pull it off I've got a few species you might want to have a go at

Mark Hall · 08-10-2008, 06:14 PM

Me too! Welcome by the way

Where are you in the UK?

Micronana · 08-19-2008, 05:48 AM

Hi 51st State
I'd be interested to hear what varieties you've got going

and Mark Hall
I'm located near Bognor Regis on the south coast.

Work to do - ho hum

Dawn

51st state · 08-20-2008, 12:46 PM

Hi Dawn
see my profile, which lists the species/varieties I'm growing

kgbenson · 08-24-2008, 05:28 PM

Quote:

Originally Posted by Mark Hall

Chong, Thanks for the link. Very interesting reading. I did think there would be more pictures after the meristem was removed showing various stages of growth.

Yeah - I just never got around to taking many or to posting them. Lemmie look back into some of my files and if I can find them I will try to get them on there.
Keith

griphuz · 08-28-2008, 10:58 AM

Maybe this article is usefull for the Ensete's?
http://www.barc.ernet.in/webpages/le.../200210-24.pdf
Regards,
Remko.

Chironex · 09-01-2008, 01:35 AM

I know that there are some of us who are doing micropropagation. I would like to see if there is any interest in combining our orders from various suppliers to buy in larger quantities. As an alternative, we could approach vendors for a Bananas.org membership discount and buy at wholesale pricing.

We could do the same with Agri-Starts, Phytotech Labs, etc. All they could do is say no. I just ordered more TC culture jars and noticed a huge discount if ordering by the hundred lot rather than 25 lot. Savings is almost 20% in just this example. Larger quantities could be greater obviously. M&S media is 12-20% lower in quantity.

Anyone interested? Let's talk about it and see if there is enough interest. We have numbers of members into this or interested in TC. Let's make use of it - a nice membership benefit in my opinion.

06-10-2008, 07:59 AM	#22 (permalink)
musa_monkey musa_monkey's Photo Gallery Location: Burnham, Bucks, UK Find me on the map! Zone: 8 Join Date: Sep 2007 Posts: 121 BananaBucks : 1,155 Feedback: 0 / 0% Thanks: 11 Thanked 23 Times in 16 Posts Welcomes: 0	Re: Tissue Culture This maybe ? Hydroponics Cloning Machines - Plantlighting Hydroponics & Grow Lights

06-10-2008, 12:15 PM	#24 (permalink)
Dean W. Dean W.'s Photo Gallery Location: Cedar Park, TX Zone: 8b Name: Dean Join Date: Sep 2007 Posts: 1,199 BananaBucks : 8,039 Feedback: 6 / 100% Thanks: 645 Thanked 222 Times in 209 Posts Welcomes: 199	Re: Tissue Culture I want to keep track of this thread. I have an Ensete perreri that might be TC material. __________________ Dean

06-28-2008, 03:06 PM	#25 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,163 BananaBucks : 557 Feedback: 5 / 100% Thanks: 1,779 Thanked 301 Times in 210 Posts Welcomes: 246	Re: Tissue Culture Dean, keep in mind that TC'ing a banana requires the original pseudostem to be destroyed. We dissect the apical meristem from the base of the pseudostem. You might be able to re-grow from the corm. There is always a risk of contamination in TC work, however small the percentage (Less than 2% hopefully), so always keep a back-up, just in case. I have several Ensete perrieri seeds in germination, so if I get a decent germination rate, I plan to TC one of them. __________________ Scot "Grow 'em if ya got 'em!"

06-28-2008, 04:24 PM	#26 (permalink)
Richard Hermitian Operator Richard's Photo Gallery Location: NW San Diego, CA Find me on the map! Zone: 9+ / 10- Name: Richard Join Date: Jan 2008 Posts: 2,370 BananaBucks : 569 Feedback: 4 / 100% Thanks: 356 Thanked 578 Times in 381 Posts Welcomes: 621	Re: Tissue Culture I thought that banana TC has been successfully initiated from growth tips and cambium material in rhizomes. Is this true and just not a preferred practice, or ? __________________ . . . http://www.plantsthatproduce.com

07-03-2008, 05:57 PM	#27 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,163 BananaBucks : 557 Feedback: 5 / 100% Thanks: 1,779 Thanked 301 Times in 210 Posts Welcomes: 246	Re: Tissue Culture I thought all might be interested to know that I have been emailing the guy who created Little Prince. It is a sport that was created in TC from 4 mutants. It is patented as a variety, so TC'ing it will require permission. I will invite him too join our forum. __________________ Scot "Grow 'em if ya got 'em!"

Members currently in the chatroom: 0
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07-04-2008, 01:23 AM	#28 (permalink)
mskitty38583 banana junkie mskitty38583 's Photo Gallery Location: sparta, tn. Find me on the map! Zone: 6-7 Name: mskitty Join Date: Nov 2007 Posts: 3,598 BananaBucks : 16,616 Feedback: 24 / 100% Thanks: 23 Thanked 462 Times in 393 Posts Welcomes: 59	Re: Tissue Culture that would be awesome scot! __________________ WELCOME TO THE GATOR NATION "kokomo" beach boys:

07-04-2008, 11:43 AM	#29 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,163 BananaBucks : 557 Feedback: 5 / 100% Thanks: 1,779 Thanked 301 Times in 210 Posts Welcomes: 246	Re: Tissue Culture Update: He just emailed me that he will be joining as soon as he gets his computer back online. Apparently a Microsoft security update has locked him out of his desktops and he is having it repaired. Bottom line is he will be joining us soon. His name is Randy, but I don't have his last name. It will be exciting to include him among our membership. It will also be helpful to anyone who develops their own sport and seeks to have it patented, from someone who knows the ropes. __________________ Scot "Grow 'em if ya got 'em!"

08-07-2008, 04:26 AM	#30 (permalink)
Micronana Location: South England Find me on the map! Name: Dawn Join Date: Aug 2008 Posts: 5 BananaBucks : 293 Feedback: 0 / 0% Thanks: 2 Thanked 0 Times in 0 Posts Welcomes: 0	Re: Tissue Culture Hi New member here, The diagram from Gabe 15 was brilliant, I am microing musa basjoo, dwarf cavendish and ensete v. maurelii (although the last 2 are new to me, and only just initiated). I have never tried being quite so brutal with them, I usually just cut them down the middle. Might try quartering some in future. By the way, I've recently read that the ensete don't produce pups spontaniously. Mine in culture have already started sprouting! I'll try damaging a few anyway to see the results. Has any one got any experience of them? Thanks Dawn

08-10-2008, 05:15 PM	#32 (permalink)
51st state Mimi's dad's grown a Nana 51st state's Photo Gallery Location: salisbury, UK Find me on the map! Zone: 8b ish Name: Kev Join Date: Nov 2006 Posts: 275 BananaBucks : 7,278 Feedback: 2 / 100% Thanks: 50 Thanked 61 Times in 44 Posts Welcomes: 0	Re: Tissue Culture Hi Dawn welcome aboard the best banana site in the world!!!!!!!!!!!!! great to see you trying TC'ing some nana's. If you can pull it off I've got a few species you might want to have a go at __________________ a>

08-10-2008, 06:14 PM	#33 (permalink)
Mark Hall Mark Mark Hall's Photo Gallery Location: Windsor u.k. Find me on the map! Name: Mark Hall Join Date: Oct 2006 Posts: 415 BananaBucks : 4,807 Feedback: 1 / 100% Thanks: 83 Thanked 57 Times in 35 Posts Welcomes: 2	Re: Tissue Culture Me too! Welcome by the way Where are you in the UK? __________________ Time Flies like an Arrow.....Fruit flies like a Banana.

08-19-2008, 05:48 AM	#34 (permalink)
Micronana Location: South England Find me on the map! Name: Dawn Join Date: Aug 2008 Posts: 5 BananaBucks : 293 Feedback: 0 / 0% Thanks: 2 Thanked 0 Times in 0 Posts Welcomes: 0	Re: Tissue Culture Hi 51st State I'd be interested to hear what varieties you've got going and Mark Hall I'm located near Bognor Regis on the south coast. Work to do - ho hum Dawn

08-20-2008, 12:46 PM	#35 (permalink)
51st state Mimi's dad's grown a Nana 51st state's Photo Gallery Location: salisbury, UK Find me on the map! Zone: 8b ish Name: Kev Join Date: Nov 2006 Posts: 275 BananaBucks : 7,278 Feedback: 2 / 100% Thanks: 50 Thanked 61 Times in 44 Posts Welcomes: 0	Re: Tissue Culture Hi Dawn see my profile, which lists the species/varieties I'm growing __________________ a>

08-28-2008, 10:58 AM	#37 (permalink)
griphuz Junior Member Location: Netherlands (Zone 8) Find me on the map! Zone: 7 - 8a Name: Remko Join Date: Sep 2006 Posts: 73 BananaBucks : 1,729 Feedback: 0 / 0% Thanks: 14 Thanked 26 Times in 12 Posts Welcomes: 212	Re: Tissue Culture Maybe this article is usefull for the Ensete's? http://www.barc.ernet.in/webpages/le.../200210-24.pdf Regards, Remko.

09-01-2008, 01:35 AM	#38 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,163 BananaBucks : 557 Feedback: 5 / 100% Thanks: 1,779 Thanked 301 Times in 210 Posts Welcomes: 246	Re: Tissue Culture I know that there are some of us who are doing micropropagation. I would like to see if there is any interest in combining our orders from various suppliers to buy in larger quantities. As an alternative, we could approach vendors for a Bananas.org membership discount and buy at wholesale pricing. We could do the same with Agri-Starts, Phytotech Labs, etc. All they could do is say no. I just ordered more TC culture jars and noticed a huge discount if ordering by the hundred lot rather than 25 lot. Savings is almost 20% in just this example. Larger quantities could be greater obviously. M&S media is 12-20% lower in quantity. Anyone interested? Let's talk about it and see if there is enough interest. We have numbers of members into this or interested in TC. Let's make use of it - a nice membership benefit in my opinion. __________________ Scot "Grow 'em if ya got 'em!"