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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 02-25-2013, 04:02 AM   #81 (permalink)
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Default Re: Tissue Culture

A cloning machine? could you further explain how it works
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Old 06-06-2016, 03:07 AM   #82 (permalink)
 
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Default Re: Tissue Culture

Quote:
Originally Posted by chong View Post
Here's one from one of our members, kgbenson:
Micropropagation of Musa
Link sent me to a small business web page..nothing about bananas
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Old 06-06-2016, 12:36 PM   #83 (permalink)
 
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Default Re: Tissue Culture

Aloha,

I have recently been allowed to use a small facility for experimenting. This man is awesome, he wants to try new things out. He has an Orchid business so my first attempt will be my Vanilla. The specimen seems to be disease free. My other thought was native bananas... maybe Ma'oli to start with, or possibly just a Mysore. My main goal to to successfully TC the Fe'i. We plan on keeping our mushrooms in his lab for safe keeping as well.

I have a lot of learning and experimenting to do... but there is a lot of information out in the world, so it will just take time.



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Old 06-06-2016, 08:18 PM   #84 (permalink)
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Default Re: Tissue Culture

Quote:
Originally Posted by chong View Post
Here's one from one of our members, kgbenson:
Micropropagation of Musa

Quote:
Originally Posted by lloydgibson1234@gmail.com View Post
Link sent me to a small business web page..nothing about bananas
When I posted that link, it went to kgbenson's website and had a full presentation of banana tissue culture, including the selection and preparation of the meristem from the corm through various stages of division. I will try to find a copy of his presentation from that link. If I can find it, I will attach it if the copy is in word or pdf format.

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Old Today, 07:34 AM   #85 (permalink)
 
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Default Re: Tissue Culture

Do you have to use a Musa pup for the donor TC. Can a young clone plant be used. I am asking because I want to try TC and I dont have any adult plants, or pups. I only have some growing clones for tissue.
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Old Today, 01:19 PM   #86 (permalink)
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Default Re: Tissue Culture

Quote:
Originally Posted by greenfumb View Post
Do you have to use a Musa pup for the donor TC. Can a young clone plant be used. I am asking because I want to try TC and I dont have any adult plants, or pups. I only have some growing clones for tissue.
If you are just experimenting, it is fine to use a young TC plant instead of a field grown sucker (if that's what you mean), just as long as it is a healthy growing plant.

If it were a serious professional project and you were producing them in high quantity, then it should be avoided. This is because there tends to be a small number of plants produced from TC which are "off-types", and the resulting plants are not true-to-type. If you use a young tissue cultured plant as starting stock, you have no guarantee the plant is true-to-type and you could be creating a bunch of off-type plants. For this reason, a well run banana tissue culture lab will only produce about 5 subculture cycles from a single sucker, and then discontinue that line and start over from a new field-grown sucker which has been verified true-to-type.
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