Tissue Culture - Page 4

rohsen · 05-30-2009, 02:09 PM

hi there here's an article on using growth retardants for cultivating Musa in temporary immersion systems, people interested in using these systems can contact me if they please

ps i just found i had already posted this paper....???!!! sorry for that

rohsen · 05-30-2009, 02:36 PM

jack draw, griphuz, chironex, after reading your discussion heres some reading material for you all

rohsen · 05-30-2009, 02:43 PM

and 2 more publications

rohsen · 06-01-2009, 04:28 AM

some clarification on these texts
alginate encapsulation is where you drop a somatic embryo in a sodium alginate solution thus creating what is known as a synthetic seed. wonderfull though complex technique.
Soma means "coming from the self" genesis "to give birth" so soma-tic embryo-genesis stands for creating embryos from the plants own cells, be they leaf, flower, stem or any other cell. (with different results )
protoplasts are basically cells with their cell walls removed allowing for "easy" in vitro crossbreeding and all sorts of other "easy" tricks (note the cynical """ marks around easy)All these techniques are pretty advanced. but it never harms to read ...right?

ps its always cool (for posting members) to ask me for specific articles, i have access to most scientific databases

alah · 07-29-2009, 02:12 AM

Quote:

Originally Posted by kgbenson

Yeah - I just never got around to taking many or to posting them. Lemmie look back into some of my files and if I can find them I will try to get them on there.
Keith

Hi, Keith. This is the kind of lecturing that I like, I need.. Please excuse me everybody else, you see, I´m areal novice. So keith, hope you´re still around and able to continue this with lecturing on the rest of the process.
Thanks, Alberto.

reenaevm · 08-10-2009, 12:32 AM

hi all,

had a query regarding multiplication of plantlets in vitro.
i have successfully initiated and got around 3-4 shoots from each initiated corm. these hav now grown into lil plantlets.

i want to know if it is possible to further take them for multiplication and how to go about with the multiplication. i kno i need to cut off all the leaves and roots but i'm confused about whether i have to discared the original corm tissue or not, the original corm has started blackening and some of the leaves have also started yellowing.

I'm a first timer with banana tc, so i'v really no idea how to go about it.
the variety i'm culturing is grand naine bananas

thanks in advance

reena

rohsen · 08-16-2009, 03:07 PM

as a rule i always reuse the parts that gave me shoots!! there may be many buds left in the part that already gave you some ... just cut of the browned bits and replant. nothing you can lose there...
regards dave

alah · 09-07-2009, 12:57 PM

Quote:

Originally Posted by chong

Here's one from one of our members, kgbenson:
Micropropagation of Musa

Help. This article seems very useful to me, but when I try to download it I get an error message saying " Address not valid". Why, Alberto.

chong · 09-07-2009, 01:41 PM

Quote:

Originally Posted by alah

Help. This article seems very useful to me, but when I try to download it I get an error message saying " Address not valid". Why, Alberto.

Check your computer's security settings. That may be preventing your computer from downloading this file. Or, you may not have enough RAM or HD memory. I just downloaded it again, just now, and I was able to save it as a *.html file. Another way that I saved it, just now also, was to highlight the entire article, and then copy and paste it on to a Word document. The latter procedure assumes that you are able to open the link to the article.

Hope that works for you.

Chong

jmoore · 09-07-2009, 02:25 PM

http___www.geocities.com_k2benson_TCoverview_TCban_TCban.pdf here's a pdf of the same article

alah · 09-07-2009, 09:01 PM

Quote:

Originally Posted by chong

Check your computer's security settings. That may be preventing your computer from downloading this file. Or, you may not have enough RAM or HD memory. I just downloaded it again, just now, and I was able to save it as a *.html file. Another way that I saved it, just now also, was to highlight the entire article, and then copy and paste it on to a Word document. The latter procedure assumes that you are able to open the link to the article.

Hope that works for you.

Chong

Thanks, Chong. Something´s gone wrong with my CPU.
It hangsup every once in a while. I´ll check tomorrow.

alah · 09-07-2009, 11:51 PM

Quote:

Originally Posted by jmoore

Attachment 1262 here's a pdf of the same article

Thanks, James. I think I´ll stick to this protocol mainly because Phytotech has a distributor at Colombia. That means they´re right next to me and I can pay in our currency ( not US$ at 600% the official exchange!)

alah · 09-08-2009, 12:41 AM

Quote:

Originally Posted by kgbenson

Yeah - I just never got around to taking many or to posting them. Lemmie look back into some of my files and if I can find them I will try to get them on there.
Keith

Excuse me Keith. I received this email, with use of Phyto Tech Lab products for banana MP. I have some doubts with their explanation, as well as your´s. Can you please check and clarify thanks, Alberto.

ligature · 02-06-2010, 04:07 AM

kindly let me know about automisation of micropropogation by using bioreactors
madhu

kamayoi · 02-07-2010, 11:43 PM

http://www.agriculture.gov.bn/DOA200...banana_TC3.pdf --> source : Brunei Agricultural research centre
banana TC..

ligature · 02-08-2010, 09:24 PM

thanks for your information kindly give some company reference who will install bioreactors for our commercial tissue culture lab in India.

Quote:

Originally Posted by kamayoi

http://www.agriculture.gov.bn/DOA200...banana_TC3.pdf --> source : Brunei Agricultural research centre
banana TC..

ligature · 02-08-2010, 09:31 PM

can any body give more info about bio-reactors technology for micro-propagation for commercial tissue culture units and even the company which supply and install equipments.

B S MADHUSUDHAN
info@ligaturebiotechnologies.com
ligature@live.in
+91 9449837234

kamayoi · 02-09-2010, 02:44 AM

Quote:

Originally Posted by ligature

thanks for your information kindly give some company reference who will install bioreactors for our commercial tissue culture lab in India.

ur welcome fren..

06-01-2009, 04:28 AM	#64 (permalink)
rohsen rohsen's Photo Gallery Location: Amsterdam, Netherlands Find me on the map! Name: Dave Rossen Join Date: Nov 2008 Posts: 32 BananaBucks : 3,251 Feedback: 0 / 0% Thanks: 8 Thanked 71 Times in 21 Posts Welcomes: 0	Re: Tissue Culture some clarification on these texts alginate encapsulation is where you drop a somatic embryo in a sodium alginate solution thus creating what is known as a synthetic seed. wonderfull though complex technique. Soma means "coming from the self" genesis "to give birth" so soma-tic embryo-genesis stands for creating embryos from the plants own cells, be they leaf, flower, stem or any other cell. (with different results ) protoplasts are basically cells with their cell walls removed allowing for "easy" in vitro crossbreeding and all sorts of other "easy" tricks (note the cynical """ marks around easy)All these techniques are pretty advanced. but it never harms to read ...right? ps its always cool (for posting members) to ask me for specific articles, i have access to most scientific databases __________________ Reality Is What You Can Get Away With!! Robert Anton Wilson Last edited by rohsen : 06-01-2009 at 10:54 AM.

08-10-2009, 12:32 AM	#66 (permalink)
reenaevm Join Date: Aug 2009 Posts: 1 BananaBucks : 79 Feedback: 0 / 0% Thanks: 0 Thanked 0 Times in 0 Posts Welcomes: 0	Re: Tissue Culture hi all, had a query regarding multiplication of plantlets in vitro. i have successfully initiated and got around 3-4 shoots from each initiated corm. these hav now grown into lil plantlets. i want to know if it is possible to further take them for multiplication and how to go about with the multiplication. i kno i need to cut off all the leaves and roots but i'm confused about whether i have to discared the original corm tissue or not, the original corm has started blackening and some of the leaves have also started yellowing. I'm a first timer with banana tc, so i'v really no idea how to go about it. the variety i'm culturing is grand naine bananas thanks in advance reena

08-16-2009, 03:07 PM	#67 (permalink)
rohsen rohsen's Photo Gallery Location: Amsterdam, Netherlands Find me on the map! Name: Dave Rossen Join Date: Nov 2008 Posts: 32 BananaBucks : 3,251 Feedback: 0 / 0% Thanks: 8 Thanked 71 Times in 21 Posts Welcomes: 0	Re: Tissue Culture as a rule i always reuse the parts that gave me shoots!! there may be many buds left in the part that already gave you some ... just cut of the browned bits and replant. nothing you can lose there... regards dave __________________ Reality Is What You Can Get Away With!! Robert Anton Wilson

09-07-2009, 02:25 PM	#70 (permalink)
jmoore <div style="font-weight: bold;"></div> jmoore 's Photo Gallery Location: Peterborough, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 196 BananaBucks : 7,377 Feedback: 0 / 0% Thanks: 25 Thanked 117 Times in 56 Posts Welcomes: 3	Re: Tissue Culture http___www.geocities.com_k2benson_TCoverview_TCban_TCban.pdf here's a pdf of the same article

02-06-2010, 04:07 AM	#74 (permalink)
ligature Join Date: Nov 2009 Posts: 3 BananaBucks : 86 Feedback: 0 / 0% Thanks: 0 Thanked 0 Times in 0 Posts Welcomes: 0	Re: Tissue Culture kindly let me know about automisation of micropropogation by using bioreactors madhu

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02-07-2010, 11:43 PM	#75 (permalink)
kamayoi Join Date: Jan 2010 Posts: 25 BananaBucks : 1,164 Feedback: 0 / 0% Thanks: 2 Thanked 40 Times in 10 Posts Welcomes: 0	Re: Tissue Culture http://www.agriculture.gov.bn/DOA200...banana_TC3.pdf --> source : Brunei Agricultural research centre banana TC..

02-08-2010, 09:31 PM	#77 (permalink)
ligature Join Date: Nov 2009 Posts: 3 BananaBucks : 86 Feedback: 0 / 0% Thanks: 0 Thanked 0 Times in 0 Posts Welcomes: 0	Re: Tissue Culture can any body give more info about bio-reactors technology for micro-propagation for commercial tissue culture units and even the company which supply and install equipments. B S MADHUSUDHAN info@ligaturebiotechnologies.com ligature@live.in +91 9449837234

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