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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 06-10-2008, 06:29 AM   #21 (permalink)
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Default Re: Tissue Culture

Quote:
Originally Posted by sandy0225 View Post
I said that last year too, though.
I did get a cloning machine though, that sounded very do-able to me. I'm supposed to get it today.
A cloning "machine"? Do you have a link to it? I would be interested to see it. What does it do?
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Old 06-10-2008, 06:59 AM   #22 (permalink)
 
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Default Re: Tissue Culture

This maybe ?

Hydroponics Cloning Machines - Plantlighting Hydroponics & Grow Lights
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Old 06-10-2008, 09:38 AM   #23 (permalink)
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Default Re: Tissue Culture

Looks like this thread is really blowing wind into some sails!!
I'm elated to see all the interest everyone has into tissue culturing and bringing some of the more rare bananas into production. I have always had an interest in TC'ing but have never taken the initiative. Perhaps watching the success of our members will help convince me to find the time and space and set it up properly.

Quote:
Originally Posted by Gabe15 View Post
Iʻll try to find some pictures, but in the meantime, I made up this diagram showing basically what happens after the initiation (when tissue culture becomes micropropagation). You will come across inconsistencies in actual practice, such as dead explants, contamination, varying degrees of proliferation, size of regenerated meristems and duration between meristem regenerations. But its generally what happens, or at least the way I do it is.

NOTE: The plants do not actually get smaller and smaller, I just only had a finite working space to show the examples.
This is great! Thanks for taking the time to create it.
BTW, I copied it over into the banana diagrams category in the gallery.

Thread stuck by the way, everyone, so we can use it as a general reference and a place to point people to.

We do have a page in the wiki about tissue culturing, it's here:
Info:Tissue Culturing - Bananas Wiki
It's fleshing out, but these things take time. Feel free to add to it, we can use both this thread and the page in the wiki as a springboard for information.

Cheers
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Old 06-10-2008, 11:15 AM   #24 (permalink)
 
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Default Re: Tissue Culture

I want to keep track of this thread. I have an Ensete perreri that might be TC material.
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Old 06-28-2008, 02:06 PM   #25 (permalink)
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Default Re: Tissue Culture

Dean, keep in mind that TC'ing a banana requires the original pseudostem to be destroyed. We dissect the apical meristem from the base of the pseudostem. You might be able to re-grow from the corm.
There is always a risk of contamination in TC work, however small the percentage (Less than 2% hopefully), so always keep a back-up, just in case. I have several Ensete perrieri seeds in germination, so if I get a decent germination rate, I plan to TC one of them.
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Old 06-28-2008, 03:24 PM   #26 (permalink)
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Default Re: Tissue Culture

I thought that banana TC has been successfully initiated from growth tips and cambium material in rhizomes. Is this true and just not a preferred practice, or ?
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Old 07-03-2008, 04:57 PM   #27 (permalink)
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Default Re: Tissue Culture

I thought all might be interested to know that I have been emailing the guy who created Little Prince. It is a sport that was created in TC from 4 mutants. It is patented as a variety, so TC'ing it will require permission. I will invite him too join our forum.
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Old 07-04-2008, 12:23 AM   #28 (permalink)
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Default Re: Tissue Culture

that would be awesome scot!
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Old 07-04-2008, 10:43 AM   #29 (permalink)
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Default Re: Tissue Culture

Update: He just emailed me that he will be joining as soon as he gets his computer back online. Apparently a Microsoft security update has locked him out of his desktops and he is having it repaired.
Bottom line is he will be joining us soon. His name is Randy, but I don't have his last name.
It will be exciting to include him among our membership. It will also be helpful to anyone who develops their own sport and seeks to have it patented, from someone who knows the ropes.
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Old 08-07-2008, 03:26 AM   #30 (permalink)
 
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Default Re: Tissue Culture

Hi New member here,
The diagram from Gabe 15 was brilliant, I am microing musa basjoo, dwarf cavendish and ensete v. maurelii (although the last 2 are new to me, and only just initiated).
I have never tried being quite so brutal with them, I usually just cut them down the middle. Might try quartering some in future.
By the way, I've recently read that the ensete don't produce pups spontaniously. Mine in culture have already started sprouting! I'll try damaging a few anyway to see the results. Has any one got any experience of them?

Thanks Dawn
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Old 08-07-2008, 07:35 AM   #31 (permalink)
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Default Re: Tissue Culture

Quote:
Originally Posted by Micronana View Post
Hi New member here,
The diagram from Gabe 15 was brilliant, I am microing musa basjoo, dwarf cavendish and ensete v. maurelii (although the last 2 are new to me, and only just initiated).
I have never tried being quite so brutal with them, I usually just cut them down the middle. Might try quartering some in future.
By the way, I've recently read that the ensete don't produce pups spontaniously. Mine in culture have already started sprouting! I'll try damaging a few anyway to see the results. Has any one got any experience of them?

Thanks Dawn
Hello and welcome Dawn,
Ensete do not naturally produce pups in the ground, but in culture they will proliferate like normal with the benzyladenine in the media.
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Old 08-10-2008, 04:15 PM   #32 (permalink)
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Default Re: Tissue Culture

Hi Dawn
welcome aboard the best banana site in the world!!!!!!!!!!!!!

great to see you trying TC'ing some nana's. If you can pull it off I've got a few species you might want to have a go at
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Old 08-10-2008, 05:14 PM   #33 (permalink)
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Default Re: Tissue Culture

Me too! Welcome by the way Where are you in the UK?
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Old 08-19-2008, 04:48 AM   #34 (permalink)
 
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Default Re: Tissue Culture

Hi 51st State
I'd be interested to hear what varieties you've got going

and Mark Hall
I'm located near Bognor Regis on the south coast.

Work to do - ho hum

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Old 08-20-2008, 11:46 AM   #35 (permalink)
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Default Re: Tissue Culture

Hi Dawn
see my profile, which lists the species/varieties I'm growing
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Old 08-24-2008, 04:28 PM   #36 (permalink)
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Default Re: Tissue Culture

Quote:
Originally Posted by Mark Hall View Post
Chong, Thanks for the link. Very interesting reading. I did think there would be more pictures after the meristem was removed showing various stages of growth.

Yeah - I just never got around to taking many or to posting them. Lemmie look back into some of my files and if I can find them I will try to get them on there.
Keith
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http://www.geocities.com/k2benson/TC...ban/TCban.html
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Old 08-28-2008, 09:58 AM   #37 (permalink)
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Default Re: Tissue Culture

Maybe this article is usefull for the Ensete's?
http://www.barc.ernet.in/webpages/le.../200210-24.pdf
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Old 09-01-2008, 12:35 AM   #38 (permalink)
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Default Re: Tissue Culture

I know that there are some of us who are doing micropropagation. I would like to see if there is any interest in combining our orders from various suppliers to buy in larger quantities. As an alternative, we could approach vendors for a Bananas.org membership discount and buy at wholesale pricing.

We could do the same with Agri-Starts, Phytotech Labs, etc. All they could do is say no. I just ordered more TC culture jars and noticed a huge discount if ordering by the hundred lot rather than 25 lot. Savings is almost 20% in just this example. Larger quantities could be greater obviously. M&S media is 12-20% lower in quantity.

Anyone interested? Let's talk about it and see if there is enough interest. We have numbers of members into this or interested in TC. Let's make use of it - a nice membership benefit in my opinion.
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Old 09-01-2008, 10:40 AM   #39 (permalink)
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Default Re: Tissue Culture

wow! the institute for advanced banana research funded and supported by bananas.org. Interesting concept! Scot every time I read one of your threads the possibilities just explode in my mind.!!!
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Old 09-02-2008, 04:33 PM   #40 (permalink)
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Default Re: Tissue Culture

agree with all that. got to be a way of having members subscribe to wanting a particular species/variety before you TC it so that you can gauge demand and have customers ready and waiting before you start. Not sure how people like agristarts would react to 'hobbyists' clubbing together though... I'm in
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