Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 09-28-2009, 09:51 PM   #1 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 480
BananaBucks : 414,468
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 696 Times in 205 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Step by Step How To Tissue Culturing Bananas

step 1. Prepare the corm
step 2 scarep out skin (sorry i dont know right term)
step 3 soak in bayklin (bleach) 25-30 minute and give it shake
sometime
step 4. rinse with sterile water 5 times
step 5 put on sterile petri dish or plate. and reary to cut. prepare the tools before like pinset, scapel/knife, bunsen fire dll
step 6. scarep out layer of stem has died (because bleach) aorund the explant
step 7 cut vertically become 2 explant
step 8 each explant put/planted in the culture bottle that has been prepared before. use MS media with 3-5 mg B enzyl amino purine (BAP)/liter solution
step 9. after 2 weeks explant growth
step 10 after 1 month explant ready for subculture
Attached Images
File Type: jpg IMGP2160 step 1.JPG (57.3 KB, 247 views)
File Type: jpg IMGP2161 step 2.JPG (61.1 KB, 252 views)
File Type: jpg IMGP2162 step 3.JPG (85.4 KB, 221 views)
File Type: jpg IMGP2163 step 4.JPG (92.0 KB, 203 views)
File Type: jpg IMGP2164 step 5.JPG (105.8 KB, 233 views)
File Type: jpg IMGP2165 step 6.JPG (66.6 KB, 224 views)
File Type: jpg IMGP2166 step 7.JPG (69.2 KB, 262 views)
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 09-29-2009, 02:54 AM   #2 (permalink)
 
browndrake's Avatar
 
Location: Northern Arizona
Zone: 7
Name: browndrake
Join Date: Jun 2009
Posts: 236
BananaBucks : 39,996
Feedback: 0 / 0%
Said "Thanks" 195 Times
Was Thanked 160 Times in 72 Posts
Said "Welcome to Bananas" 140 Times
Default Re: Step by Step How To Tissue Culturing Bananas

thanks...

You have done a great job in your lab. Those of us that are learning appreciate your posts

aaron
__________________
Click for Fredonia, Arizona Forecast
browndrake is offline   Reply With Quote Send A Private Message To browndrake
Old 09-29-2009, 09:35 AM   #3 (permalink)
xmoose
 
xmoose's Avatar
 
Location: Marin county, California
Zone: 9 - 10
Name: Chris
Join Date: May 2009
Posts: 505
BananaBucks : 4,516
Feedback: 1 / 100%
Said "Thanks" 27 Times
Was Thanked 20 Times in 19 Posts
Said "Welcome to Bananas" 27 Times
Default Re: Step by Step How To Tissue Culturing Bananas

This is Excellent information for people like me.
Thank you
xmoose is offline   Reply With Quote Send A Private Message To xmoose
Old 09-29-2009, 02:10 PM   #4 (permalink)
Got pink bananas?
 
austinl01's Avatar
 
Location: Little Rock, AR
Zone: 7b/8a Line
Name: Austin
Join Date: Mar 2006
Posts: 1,288
BananaBucks : 161,506
Feedback: 13 / 100%
Said "Thanks" 1,590 Times
Was Thanked 907 Times in 397 Posts
Said "Welcome to Bananas" 507 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Thanks for the pictures. I have always wondered how it was done.
__________________
Austin
Arkansas River Valley

Average January Low: 31°F, Average August High: 92°F
Extreme Winter Lows: 5-15°F, Extreme Summer Highs: 100-112°F, Annual Precipitation: 52 inches
austinl01 is offline   Reply With Quote Send A Private Message To austinl01
Old 09-29-2009, 07:47 PM   #5 (permalink)
 
r3tic's Avatar
 
Location: Drexel Hill, PA
Zone: 7-8
Name: Dave
Join Date: Dec 2008
Posts: 216
BananaBucks : 45,280
Feedback: 2 / 100%
Said "Thanks" 531 Times
Was Thanked 123 Times in 71 Posts
Said "Welcome to Bananas" 306 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Could you post photos of the subculture process please. Thanks for the information.
__________________
Click for Drexel Hill, Pennsylvania Forecast
r3tic is offline   Reply With Quote Send A Private Message To r3tic
Sponsors

Old 09-29-2009, 08:05 PM   #6 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 480
BananaBucks : 414,468
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 696 Times in 205 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: Step by Step How To Tissue Culturing Bananas

ok I will post soon.
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Old 09-29-2009, 09:01 PM   #7 (permalink)
 
Blake09's Avatar
 
Zone: Zone 7B
Name: Blake
Join Date: Aug 2009
Posts: 1,237
BananaBucks : 109,334
Feedback: 2 / 100%
Said "Thanks" 1,424 Times
Was Thanked 1,105 Times in 448 Posts
Said "Welcome to Bananas" 328 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Wow thanks! You saved me hours of reading mile long page after page...
__________________

A banana plant in the autumn gale -
I listen to the dripping of rain
Into a basin at night.
~ Basho Matsuo (1644- 1694)
Blake09 is offline   Reply With Quote Send A Private Message To Blake09
Old 09-29-2009, 09:16 PM   #8 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 480
BananaBucks : 414,468
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 696 Times in 205 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: Step by Step How To Tissue Culturing Bananas

dear blake
Yes if we reading textbook we needs much time. but what i post is very simple and practically. ihope you succes star with TC
regards
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Said thanks:
Old 09-29-2009, 09:27 PM   #9 (permalink)
 
Jananas Bananas's Avatar
 
Location: Austin, Texas
Zone: 8b/9a
Name: JaNan
Join Date: Jun 2009
Posts: 4,438
BananaBucks : 390,177
Feedback: 1 / 100%
Said "Thanks" 3,341 Times
Was Thanked 7,059 Times in 2,683 Posts
Said "Welcome to Bananas" 133 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Your very appreciated! ~JaNan
__________________
Jananas Bananas
Jananas Bananas is offline   Reply With Quote Send A Private Message To Jananas Bananas
Old 09-29-2009, 10:24 PM   #10 (permalink)
 
ArchAngeL01's Avatar
 
Location: Greenville, SC
Zone: 8A
Join Date: Mar 2009
Posts: 849
BananaBucks : 142,122
Feedback: 4 / 83%
Said "Thanks" 104 Times
Was Thanked 433 Times in 165 Posts
Said "Welcome to Bananas" 3 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Thanks so much for this thread! it is simple and helps me out alot Novi roxx! hehe!
__________________
Brad
ArchAngeL01 is offline   Reply With Quote Send A Private Message To ArchAngeL01
Old 09-30-2009, 12:38 AM   #11 (permalink)
Junior Member
 
Location: West Palm Beach
Zone: 10
Name: james
Join Date: Jul 2007
Posts: 506
BananaBucks : 9,458
Feedback: 0 / 0%
Said "Thanks" 21 Times
Was Thanked 105 Times in 63 Posts
Said "Welcome to Bananas" 4 Times
Default Re: Step by Step How To Tissue Culturing Bananas

kool i hope some day to do it too.
frog7994 is offline   Reply With Quote Send A Private Message To frog7994
Old 03-23-2012, 02:13 AM   #12 (permalink)
 
Join Date: Mar 2010
Posts: 3
BananaBucks : 2,555
Feedback: 0 / 0%
Said "Thanks" 1 Times
Was Thanked 3 Times in 2 Posts
Said "Welcome to Bananas" 2 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Dear Novi,

Thank you for your step by step guide on TC of bananas.

I am new in this forum with some basic knowledge of TC and was looking for help on doing TC of banana at home.

Based on your step by step guide I have some questions:-


1. What is the ideal length and diameter of the suckers ?

2. Once I get the suckers (say from site of cultivation) , how long can they be stored at home before TC ?

3. I guess I may have to store it minimum 1 day at home before doing TC. How should I store them during this intermediate time ?

4. Is there any basic guidelines on preparing the crom ? Like how much to cut from the base and how much to cut from the top part (process between image 1 to image 2)?

5. What should be the ideal / average size of the prepared crom (step 1,image 2 )?

6. If I am doing TC of more than 1 sucker at one go (since I will prepare a min. 500ml or 1lit media), what is the sequence for sterilization ?
Example :- If I have 10 suckers, do I need 10 different bottles of bleach and 10 different bottles of sterile water ?
Or should I put all 10 into one large container containing bleach and another containg sterile water ?

7. Which part of the sterilization process can be done outside the hood and which part inside the hood ?

8. What should be the ideal / average size of explant before dividing into two (after step 6, image 6)?


I am not clear regarding the above. Clarifications on the above doubts would be of great help.

Thanks in advance.

Regards,
Amit


Quote:
Originally Posted by novisyatria View Post
step 1. Prepare the corm
step 2 scarep out skin (sorry i dont know right term)
step 3 soak in bayklin (bleach) 25-30 minute and give it shake
sometime
step 4. rinse with sterile water 5 times
step 5 put on sterile petri dish or plate. and reary to cut. prepare the tools before like pinset, scapel/knife, bunsen fire dll
step 6. scarep out layer of stem has died (because bleach) aorund the explant
step 7 cut vertically become 2 explant
step 8 each explant put/planted in the culture bottle that has been prepared before. use MS media with 3-5 mg B enzyl amino purine (BAP)/liter solution
step 9. after 2 weeks explant growth
step 10 after 1 month explant ready for subculture
ab2710 is offline   Reply With Quote Send A Private Message To ab2710
Old 04-10-2012, 08:35 PM   #13 (permalink)
Tissue Culturist
 
novisyatria's Avatar
 
Location: Jakarta, Indonesia
Name: Novi Syatria
Join Date: Sep 2009
Posts: 480
BananaBucks : 414,468
Feedback: 0 / 0%
Said "Thanks" 15 Times
Was Thanked 696 Times in 205 Posts
Said "Welcome to Bananas" 0 Times
Send a message via Yahoo to novisyatria
Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by ab2710 View Post
Dear Novi,

Thank you for your step by step guide on TC of bananas.

I am new in this forum with some basic knowledge of TC and was looking for help on doing TC of banana at home.

Based on your step by step guide I have some questions:-


1. What is the ideal length and diameter of the suckers ?

2. Once I get the suckers (say from site of cultivation) , how long can they be stored at home before TC ?

3. I guess I may have to store it minimum 1 day at home before doing TC. How should I store them during this intermediate time ?

4. Is there any basic guidelines on preparing the crom ? Like how much to cut from the base and how much to cut from the top part (process between image 1 to image 2)?

5. What should be the ideal / average size of the prepared crom (step 1,image 2 )?

6. If I am doing TC of more than 1 sucker at one go (since I will prepare a min. 500ml or 1lit media), what is the sequence for sterilization ?
Example :- If I have 10 suckers, do I need 10 different bottles of bleach and 10 different bottles of sterile water ?
Or should I put all 10 into one large container containing bleach and another containg sterile water ?

7. Which part of the sterilization process can be done outside the hood and which part inside the hood ?

8. What should be the ideal / average size of explant before dividing into two (after step 6, image 6)?


I am not clear regarding the above. Clarifications on the above doubts would be of great help.

Thanks in advance.

Regards,
Amit
hi
1. suckers? you mean corm ? or plantlets?
2. we can keep corm as long as its still good about aweek
3. keep it at temperature room and dry
4. no yet
5. about 5-8 cm diameter
6. i have 2 corm in one bootle so more economic
7. stage 1-2 outside hood but mext must do in hood/LAminar
8. about 3 cm
sorry my english not well. hope you succes with your TC
__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com
novisyatria is offline   Reply With Quote Send A Private Message To novisyatria
Said thanks:
Old 04-22-2012, 10:05 PM   #14 (permalink)
 
Join Date: Apr 2012
Posts: 4
BananaBucks : 2,915
Feedback: 0 / 0%
Said "Thanks" 1 Times
Was Thanked 1 Time in 1 Post
Said "Welcome to Bananas" 1 Times
Default Re: Step by Step How To Tissue Culturing Bananas

I'm new here, and read everything I could here, but still don't see the actual step by step with explicit instructions and details. is this the place to find it ? or do I need to buy a manual......
fruitguy@cox.net is offline   Reply With Quote Send A Private Message To fruitguy@cox.net
Old 04-22-2012, 10:12 PM   #15 (permalink)
Banned
 
Location: San Diego
Zone: 9-11
Name: Tony
Join Date: Apr 2008
Posts: 18,429
BananaBucks : 938,199
Feedback: 8 / 100%
Said "Thanks" 3,210 Times
Was Thanked 20,591 Times in 7,760 Posts
Said "Welcome to Bananas" 2,716 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by fruitguy@cox.net View Post
I'm new here, and read everything I could here, but still don't see the actual step by step with explicit instructions and details. is this the place to find it ? or do I need to buy a manual......
Simply Hydroponics & Organics: Step by Step Video for Tissue Culture


Simply Hydroponics Step by Step Tissue Culture part 1 | Household Hydroponics


Last edited by sunfish : 04-22-2012 at 10:18 PM.
sunfish is offline   Reply With Quote Send A Private Message To sunfish
Old 04-22-2012, 11:16 PM   #16 (permalink)
 
Join Date: Apr 2012
Posts: 4
BananaBucks : 2,915
Feedback: 0 / 0%
Said "Thanks" 1 Times
Was Thanked 1 Time in 1 Post
Said "Welcome to Bananas" 1 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Good videos about plant cutting tissue culture, but not bananas
fruitguy@cox.net is offline   Reply With Quote Send A Private Message To fruitguy@cox.net
Said thanks:

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 04-22-2012, 11:20 PM   #17 (permalink)
Banned
 
Location: San Diego
Zone: 9-11
Name: Tony
Join Date: Apr 2008
Posts: 18,429
BananaBucks : 938,199
Feedback: 8 / 100%
Said "Thanks" 3,210 Times
Was Thanked 20,591 Times in 7,760 Posts
Said "Welcome to Bananas" 2,716 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Quote:
Originally Posted by fruitguy@cox.net View Post
Good videos about plant cutting tissue culture, but not bananas
It's a start
sunfish is offline   Reply With Quote Send A Private Message To sunfish
Said thanks:
Old 04-23-2012, 01:12 AM   #18 (permalink)
 
jmoore's Avatar
 
Location: Lowestoft, UK
Zone: 8a
Name: James
Join Date: Aug 2008
Posts: 766
BananaBucks : 118,585
Feedback: 4 / 100%
Said "Thanks" 192 Times
Was Thanked 799 Times in 373 Posts
Said "Welcome to Bananas" 71 Times
Default Re: Step by Step How To Tissue Culturing Bananas

There is a book you can buy, it's called 'plants in a test tube' or something lik that, but again doesn't specifically cover bananas. You need to read the threads and a few scientific papers and kind of go from there. This section is full of excellent instructions on how to tissue culture bananas, you just have to look for it.
jmoore is offline   Reply With Quote Send A Private Message To jmoore
Old 04-23-2012, 06:52 AM   #19 (permalink)
Banned
 
Location: San Diego
Zone: 9-11
Name: Tony
Join Date: Apr 2008
Posts: 18,429
BananaBucks : 938,199
Feedback: 8 / 100%
Said "Thanks" 3,210 Times
Was Thanked 20,591 Times in 7,760 Posts
Said "Welcome to Bananas" 2,716 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Tissue culture techniques of Banana

https://docs.google.com/viewer?a=v&q...92RBi_mkW_wHXg

http://www.academicjournals.org/ajb/...%20et%20al.pdf

Last edited by sunfish : 04-23-2012 at 07:17 AM.
sunfish is offline   Reply With Quote Send A Private Message To sunfish
Old 04-23-2012, 03:00 PM   #20 (permalink)
 
kaczercat's Avatar
 
Location: Southern ON
Zone: 5b 9b
Name: Matt
Join Date: Jun 2008
Posts: 1,874
BananaBucks : 219,365
Feedback: 3 / 100%
Said "Thanks" 3,116 Times
Was Thanked 2,111 Times in 750 Posts
Said "Welcome to Bananas" 11 Times
Default Re: Step by Step How To Tissue Culturing Bananas

Thanks, great thread!
__________________
Matt
kaczercat is offline   Reply With Quote Send A Private Message To kaczercat
Sponsors

Reply   Email this Page Email this Page






Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Tissue culturing resource materials Jack Daw Tissue Culturing & Other Propagation Techniques of Banana Plants 17 09-14-2012 12:29 PM
Big step forward Dalmatiansoap European Section 2 08-07-2009 04:34 AM
New to the forum - (Tissue Culturing Banana Plants) MurCurY Member Introductions 11 02-27-2009 04:28 PM
Two new forums today! Tissue Culturing Banana Plants and a new recipe forum! MediaHound Bananas.org Site News 12 07-11-2008 01:41 PM
New to the forum - (Tissue Culturing Banana Plants) Member Introductions 0 01-01-1970 12:00 AM


All times are GMT -5. The time now is 10:35 AM.





All content © Bananas.org & the respective author.