Questions about media
 

Hi everyone,
I am a horticulture major at Penn State and would like to start experimenting with banana tissue culture. I have a few questions. First, when tissue culturing bananas, do you tissue culture the explants in different media for different stages (for example is it necessary to use one media for shoots and then transfer them into another media for roots?) or can you simply keep them in the same media for shoot and root growth? Also, does anyone have an easy to follow recipe for making media? Thanks
-Dave

Re: Questions about media
 

Dear Dave
For bananas, just using same media MS + BAP 3-5 mg/l. banans dont need auxin because can make rooting wihtout auxin. you can ask me about TC bananas in this thread or PM me.

regards from jakarta

Re: Questions about media
 

Attachment 1349

This has a good recipe for media, I'll be trying this method in a couple of weeks in fact.